Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21285

Experiment: L-Lysine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21275 and BWI76_RS21280 overlap by 4 nucleotidesBWI76_RS21280 and BWI76_RS21285 are separated by 19 nucleotidesBWI76_RS21285 and BWI76_RS21290 are separated by 209 nucleotides BWI76_RS21275: BWI76_RS21275 - lytic transglycosylase F, at 4,456,991 to 4,458,607 _RS21275 BWI76_RS21280: BWI76_RS21280 - tRNA adenosine(34) deaminase TadA, at 4,458,604 to 4,459,149 _RS21280 BWI76_RS21285: BWI76_RS21285 - HAD family hydrolase, at 4,459,169 to 4,459,804 _RS21285 BWI76_RS21290: BWI76_RS21290 - DNA-binding transcriptional regulator, at 4,460,014 to 4,460,862 _RS21290 Position (kb) 4459 4460Strain fitness (log2 ratio) -1 0 1at 4458.374 kb on + strand, within BWI76_RS21275at 4458.380 kb on + strand, within BWI76_RS21275at 4458.382 kb on - strand, within BWI76_RS21275at 4458.492 kb on - strandat 4458.607 kb on + strandat 4459.171 kb on + strandat 4459.398 kb on + strand, within BWI76_RS21285at 4459.745 kb on - strandat 4459.988 kb on + strandat 4459.996 kb on - strandat 4460.011 kb on + strandat 4460.045 kb on + strandat 4460.053 kb on - strandat 4460.053 kb on - strandat 4460.256 kb on + strand, within BWI76_RS21290at 4460.256 kb on + strand, within BWI76_RS21290at 4460.333 kb on - strand, within BWI76_RS21290at 4460.531 kb on + strand, within BWI76_RS21290at 4460.579 kb on - strand, within BWI76_RS21290at 4460.748 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine (N)
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4,458,374 + BWI76_RS21275 0.86 -1.2
4,458,380 + BWI76_RS21275 0.86 -0.2
4,458,382 - BWI76_RS21275 0.86 -1.0
4,458,492 - -0.1
4,458,607 + +0.5
4,459,171 + +1.4
4,459,398 + BWI76_RS21285 0.36 -1.2
4,459,745 - -0.5
4,459,988 + -0.2
4,459,996 - +0.6
4,460,011 + +0.6
4,460,045 + +0.2
4,460,053 - -0.7
4,460,053 - -0.1
4,460,256 + BWI76_RS21290 0.29 +1.7
4,460,256 + BWI76_RS21290 0.29 +0.4
4,460,333 - BWI76_RS21290 0.38 -0.1
4,460,531 + BWI76_RS21290 0.61 -0.1
4,460,579 - BWI76_RS21290 0.67 +0.2
4,460,748 - +1.2

Or see this region's nucleotide sequence