Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20195

Experiment: L-Lysine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20185 and BWI76_RS20190 are separated by 15 nucleotidesBWI76_RS20190 and BWI76_RS20195 overlap by 23 nucleotidesBWI76_RS20195 and BWI76_RS20200 are separated by 463 nucleotides BWI76_RS20185: BWI76_RS20185 - NADH-quinone oxidoreductase subunit B, at 4,230,862 to 4,231,536 _RS20185 BWI76_RS20190: BWI76_RS20190 - NADH-quinone oxidoreductase subunit A, at 4,231,552 to 4,231,998 _RS20190 BWI76_RS20195: BWI76_RS20195 - hypothetical protein, at 4,231,976 to 4,232,186 _RS20195 BWI76_RS20200: BWI76_RS20200 - transcriptional regulator LrhA, at 4,232,650 to 4,233,576 _RS20200 Position (kb) 4231 4232 4233Strain fitness (log2 ratio) -1 0 1 2at 4231.087 kb on + strand, within BWI76_RS20185at 4231.302 kb on - strandat 4231.557 kb on + strandat 4231.565 kb on - strandat 4231.591 kb on - strandat 4231.657 kb on - strand, within BWI76_RS20190at 4231.908 kb on + strand, within BWI76_RS20190at 4231.926 kb on - strand, within BWI76_RS20190at 4232.281 kb on - strandat 4232.334 kb on - strandat 4232.434 kb on - strandat 4232.691 kb on - strandat 4232.705 kb on - strandat 4232.874 kb on + strand, within BWI76_RS20200at 4232.962 kb on - strand, within BWI76_RS20200at 4232.962 kb on - strand, within BWI76_RS20200at 4233.128 kb on - strand, within BWI76_RS20200at 4233.134 kb on + strand, within BWI76_RS20200at 4233.135 kb on - strand, within BWI76_RS20200

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lysine (N)
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4,231,087 + BWI76_RS20185 0.33 -1.0
4,231,302 - +2.2
4,231,557 + -0.5
4,231,565 - +1.5
4,231,591 - -0.5
4,231,657 - BWI76_RS20190 0.23 -0.3
4,231,908 + BWI76_RS20190 0.80 -0.5
4,231,926 - BWI76_RS20190 0.84 -0.2
4,232,281 - +0.0
4,232,334 - +0.6
4,232,434 - -0.1
4,232,691 - -0.5
4,232,705 - +0.2
4,232,874 + BWI76_RS20200 0.24 +0.2
4,232,962 - BWI76_RS20200 0.34 -1.0
4,232,962 - BWI76_RS20200 0.34 -0.5
4,233,128 - BWI76_RS20200 0.52 +1.3
4,233,134 + BWI76_RS20200 0.52 -1.1
4,233,135 - BWI76_RS20200 0.52 +0.6

Or see this region's nucleotide sequence