Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4734 and BT4735 are separated by 558 nucleotides BT4735 and BT4736 are separated by 213 nucleotides BT4736 and BT4737 overlap by 8 nucleotides
BT4734: BT4734 - conserved hypothetical protein (NCBI ptt file), at 6,210,159 to 6,212,225
BT4734
BT4735: BT4735 - hypothetical protein (NCBI ptt file), at 6,212,784 to 6,213,287
BT4735
BT4736: BT4736 - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 6,213,501 to 6,213,950
BT4736
BT4737: BT4737 - pyruvate formate-lyase activating enzyme (NCBI ptt file), at 6,213,943 to 6,214,671
BT4737
Position (kb)
6212
6213
6214 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6211.957 kb on + strand, within BT4734 at 6212.187 kb on - strand at 6212.187 kb on - strand at 6212.233 kb on - strand at 6212.287 kb on - strand at 6212.341 kb on - strand at 6212.353 kb on + strand at 6212.358 kb on + strand at 6212.359 kb on - strand at 6212.469 kb on + strand at 6212.470 kb on - strand at 6212.474 kb on - strand at 6212.610 kb on - strand at 6212.754 kb on - strand at 6212.856 kb on + strand, within BT4735 at 6212.924 kb on + strand, within BT4735 at 6212.924 kb on + strand, within BT4735 at 6213.279 kb on - strand at 6213.322 kb on + strand at 6213.344 kb on - strand at 6213.361 kb on - strand at 6213.443 kb on + strand at 6213.448 kb on + strand at 6213.457 kb on + strand at 6213.621 kb on + strand, within BT4736 at 6213.624 kb on + strand, within BT4736 at 6213.625 kb on - strand, within BT4736 at 6213.625 kb on - strand, within BT4736 at 6213.627 kb on - strand, within BT4736 at 6213.627 kb on - strand, within BT4736 at 6213.669 kb on - strand, within BT4736 at 6213.710 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.716 kb on - strand, within BT4736 at 6213.720 kb on + strand, within BT4736 at 6213.764 kb on + strand, within BT4736 at 6213.764 kb on + strand, within BT4736 at 6213.831 kb on - strand, within BT4736 at 6213.836 kb on - strand, within BT4736 at 6213.836 kb on - strand, within BT4736 at 6213.871 kb on - strand, within BT4736 at 6213.884 kb on - strand, within BT4736 at 6213.902 kb on + strand, within BT4736 at 6213.944 kb on + strand at 6213.948 kb on + strand at 6213.960 kb on + strand at 6213.968 kb on + strand at 6214.037 kb on + strand, within BT4737 at 6214.038 kb on - strand, within BT4737 at 6214.062 kb on + strand, within BT4737 at 6214.062 kb on + strand, within BT4737 at 6214.083 kb on - strand, within BT4737 at 6214.167 kb on - strand, within BT4737 at 6214.178 kb on - strand, within BT4737
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 6,211,957 + BT4734 0.87 -2.3 6,212,187 - -2.5 6,212,187 - -2.2 6,212,233 - -1.7 6,212,287 - -4.3 6,212,341 - -3.2 6,212,353 + -0.1 6,212,358 + -3.4 6,212,359 - -2.4 6,212,469 + -2.8 6,212,470 - +2.8 6,212,474 - -1.6 6,212,610 - -1.7 6,212,754 - -3.7 6,212,856 + BT4735 0.14 -3.8 6,212,924 + BT4735 0.28 -1.1 6,212,924 + BT4735 0.28 -3.3 6,213,279 - -0.5 6,213,322 + -1.1 6,213,344 - -2.5 6,213,361 - -3.2 6,213,443 + -3.3 6,213,448 + -3.1 6,213,457 + +0.9 6,213,621 + BT4736 0.27 -4.3 6,213,624 + BT4736 0.27 +1.9 6,213,625 - BT4736 0.28 +2.9 6,213,625 - BT4736 0.28 +1.7 6,213,627 - BT4736 0.28 -2.2 6,213,627 - BT4736 0.28 -1.3 6,213,669 - BT4736 0.37 -2.5 6,213,710 - BT4736 0.46 -1.3 6,213,716 - BT4736 0.48 -1.1 6,213,716 - BT4736 0.48 +2.1 6,213,716 - BT4736 0.48 -1.9 6,213,720 + BT4736 0.49 +1.9 6,213,764 + BT4736 0.58 -3.5 6,213,764 + BT4736 0.58 -1.5 6,213,831 - BT4736 0.73 +0.5 6,213,836 - BT4736 0.74 -2.3 6,213,836 - BT4736 0.74 -2.0 6,213,871 - BT4736 0.82 +2.2 6,213,884 - BT4736 0.85 +2.7 6,213,902 + BT4736 0.89 -2.8 6,213,944 + -2.3 6,213,948 + -2.7 6,213,960 + -2.9 6,213,968 + +1.0 6,214,037 + BT4737 0.13 -0.9 6,214,038 - BT4737 0.13 -3.6 6,214,062 + BT4737 0.16 +1.4 6,214,062 + BT4737 0.16 -0.1 6,214,083 - BT4737 0.19 -3.8 6,214,167 - BT4737 0.31 -3.2 6,214,178 - BT4737 0.32 -4.0
Or see this region's nucleotide sequence