Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4594 and BT4595 are separated by 24 nucleotides BT4595 and BT4596 are separated by 221 nucleotides BT4596 and BT4597 are separated by 59 nucleotides BT4597 and BT4598 are separated by 223 nucleotides BT4598 and BT4599 are separated by 57 nucleotides
BT4594: BT4594 - putative dephospho-CoA kinase (NCBI ptt file), at 6,015,273 to 6,015,887
BT4594
BT4595: BT4595 - hypothetical protein (NCBI ptt file), at 6,015,912 to 6,016,346
BT4595
BT4596: BT4596 - hypothetical protein (NCBI ptt file), at 6,016,568 to 6,016,816
BT4596
BT4597: BT4597 - endopeptidase Clp ATP-binding chain B (NCBI ptt file), at 6,016,876 to 6,019,464
BT4597
BT4598: BT4598 - hypothetical protein (NCBI ptt file), at 6,019,688 to 6,020,272
BT4598
BT4599: BT4599 - putative transcriptional regulator (NCBI ptt file), at 6,020,330 to 6,021,226
BT4599
Position (kb)
6016
6017
6018
6019
6020 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 6015.885 kb on + strand at 6015.954 kb on + strand at 6016.034 kb on + strand, within BT4595 at 6016.063 kb on + strand, within BT4595 at 6016.064 kb on + strand, within BT4595 at 6016.064 kb on - strand, within BT4595 at 6016.073 kb on + strand, within BT4595 at 6016.091 kb on - strand, within BT4595 at 6016.098 kb on + strand, within BT4595 at 6016.116 kb on - strand, within BT4595 at 6016.181 kb on - strand, within BT4595 at 6016.201 kb on + strand, within BT4595 at 6016.263 kb on + strand, within BT4595 at 6016.299 kb on - strand, within BT4595 at 6016.375 kb on + strand at 6016.432 kb on + strand at 6016.509 kb on - strand at 6016.511 kb on - strand at 6016.551 kb on + strand at 6016.575 kb on + strand at 6016.651 kb on + strand, within BT4596 at 6016.652 kb on - strand, within BT4596 at 6016.744 kb on + strand, within BT4596 at 6016.748 kb on + strand, within BT4596 at 6016.748 kb on + strand, within BT4596 at 6016.748 kb on + strand, within BT4596 at 6016.748 kb on + strand, within BT4596 at 6016.748 kb on + strand, within BT4596 at 6016.749 kb on - strand, within BT4596 at 6016.749 kb on - strand, within BT4596 at 6016.749 kb on - strand, within BT4596 at 6016.772 kb on - strand, within BT4596 at 6016.802 kb on + strand at 6016.802 kb on + strand at 6016.803 kb on - strand at 6016.814 kb on + strand at 6016.815 kb on - strand at 6016.867 kb on - strand at 6016.952 kb on - strand at 6017.027 kb on + strand at 6017.028 kb on - strand at 6017.028 kb on - strand at 6017.182 kb on - strand, within BT4597 at 6017.216 kb on - strand, within BT4597 at 6017.220 kb on + strand, within BT4597 at 6017.221 kb on - strand, within BT4597 at 6017.393 kb on - strand, within BT4597 at 6017.460 kb on - strand, within BT4597 at 6017.572 kb on - strand, within BT4597 at 6017.577 kb on + strand, within BT4597 at 6017.800 kb on - strand, within BT4597 at 6017.901 kb on + strand, within BT4597 at 6017.901 kb on + strand, within BT4597 at 6017.935 kb on - strand, within BT4597 at 6018.205 kb on + strand, within BT4597 at 6018.264 kb on + strand, within BT4597 at 6018.268 kb on + strand, within BT4597 at 6018.349 kb on + strand, within BT4597 at 6018.359 kb on - strand, within BT4597 at 6018.383 kb on - strand, within BT4597 at 6018.383 kb on - strand, within BT4597 at 6018.492 kb on + strand, within BT4597 at 6018.620 kb on - strand, within BT4597 at 6018.643 kb on - strand, within BT4597 at 6018.653 kb on - strand, within BT4597 at 6018.837 kb on + strand, within BT4597 at 6018.837 kb on + strand, within BT4597 at 6018.837 kb on + strand, within BT4597 at 6018.981 kb on - strand, within BT4597 at 6019.124 kb on - strand, within BT4597 at 6019.140 kb on + strand, within BT4597 at 6019.182 kb on - strand, within BT4597 at 6019.226 kb on + strand at 6019.226 kb on + strand at 6019.249 kb on - strand at 6019.249 kb on - strand at 6019.279 kb on + strand at 6019.456 kb on - strand at 6019.500 kb on - strand at 6019.560 kb on - strand at 6019.676 kb on + strand at 6019.697 kb on - strand at 6019.825 kb on + strand, within BT4598 at 6019.826 kb on - strand, within BT4598 at 6019.923 kb on + strand, within BT4598 at 6019.982 kb on + strand, within BT4598 at 6019.992 kb on - strand, within BT4598 at 6020.218 kb on + strand at 6020.270 kb on + strand at 6020.365 kb on - strand at 6020.434 kb on + strand, within BT4599 at 6020.435 kb on - strand, within BT4599 at 6020.453 kb on + strand, within BT4599
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 6,015,885 + -1.8 6,015,954 + -1.5 6,016,034 + BT4595 0.28 +4.0 6,016,063 + BT4595 0.35 +1.5 6,016,064 + BT4595 0.35 -1.6 6,016,064 - BT4595 0.35 -2.0 6,016,073 + BT4595 0.37 -1.4 6,016,091 - BT4595 0.41 +1.4 6,016,098 + BT4595 0.43 -1.3 6,016,116 - BT4595 0.47 +2.9 6,016,181 - BT4595 0.62 -1.7 6,016,201 + BT4595 0.66 -1.4 6,016,263 + BT4595 0.81 -0.1 6,016,299 - BT4595 0.89 +1.0 6,016,375 + +4.1 6,016,432 + +1.1 6,016,509 - +1.5 6,016,511 - -4.6 6,016,551 + +2.5 6,016,575 + -2.1 6,016,651 + BT4596 0.33 -1.1 6,016,652 - BT4596 0.34 -2.6 6,016,744 + BT4596 0.71 -3.6 6,016,748 + BT4596 0.72 -3.1 6,016,748 + BT4596 0.72 -2.8 6,016,748 + BT4596 0.72 -0.7 6,016,748 + BT4596 0.72 +1.2 6,016,748 + BT4596 0.72 -2.8 6,016,749 - BT4596 0.73 -0.1 6,016,749 - BT4596 0.73 +1.5 6,016,749 - BT4596 0.73 +1.5 6,016,772 - BT4596 0.82 -0.1 6,016,802 + +1.5 6,016,802 + -2.4 6,016,803 - -2.1 6,016,814 + +1.8 6,016,815 - -0.9 6,016,867 - -0.5 6,016,952 - -1.7 6,017,027 + -0.6 6,017,028 - +1.3 6,017,028 - +0.3 6,017,182 - BT4597 0.12 -0.3 6,017,216 - BT4597 0.13 +3.1 6,017,220 + BT4597 0.13 -1.1 6,017,221 - BT4597 0.13 -1.4 6,017,393 - BT4597 0.20 +0.4 6,017,460 - BT4597 0.23 -2.5 6,017,572 - BT4597 0.27 -1.9 6,017,577 + BT4597 0.27 -3.4 6,017,800 - BT4597 0.36 -0.8 6,017,901 + BT4597 0.40 -0.9 6,017,901 + BT4597 0.40 -0.7 6,017,935 - BT4597 0.41 +2.9 6,018,205 + BT4597 0.51 +3.1 6,018,264 + BT4597 0.54 -2.6 6,018,268 + BT4597 0.54 -2.0 6,018,349 + BT4597 0.57 +2.1 6,018,359 - BT4597 0.57 +1.9 6,018,383 - BT4597 0.58 +2.9 6,018,383 - BT4597 0.58 +2.1 6,018,492 + BT4597 0.62 -1.4 6,018,620 - BT4597 0.67 +2.6 6,018,643 - BT4597 0.68 +3.1 6,018,653 - BT4597 0.69 -1.9 6,018,837 + BT4597 0.76 -1.7 6,018,837 + BT4597 0.76 -2.1 6,018,837 + BT4597 0.76 +2.9 6,018,981 - BT4597 0.81 +3.1 6,019,124 - BT4597 0.87 -0.1 6,019,140 + BT4597 0.87 -0.8 6,019,182 - BT4597 0.89 -1.7 6,019,226 + -0.7 6,019,226 + +2.2 6,019,249 - -2.4 6,019,249 - -1.1 6,019,279 + -0.1 6,019,456 - +0.9 6,019,500 - -2.9 6,019,560 - -5.2 6,019,676 + -2.4 6,019,697 - -1.4 6,019,825 + BT4598 0.23 -2.4 6,019,826 - BT4598 0.24 -1.2 6,019,923 + BT4598 0.40 +2.3 6,019,982 + BT4598 0.50 -2.6 6,019,992 - BT4598 0.52 -1.5 6,020,218 + -2.1 6,020,270 + -1.1 6,020,365 - -1.0 6,020,434 + BT4599 0.12 -3.6 6,020,435 - BT4599 0.12 -2.8 6,020,453 + BT4599 0.14 +0.9
Or see this region's nucleotide sequence