Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4247 and BT4248 are separated by 50 nucleotides BT4248 and BT4249 are separated by 266 nucleotides BT4249 and BT4250 are separated by 129 nucleotides BT4250 and BT4251 are separated by 64 nucleotides
BT4247: BT4247 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,596,039 to 5,599,392
BT4247
BT4248: BT4248 - putative anti-sigma factor (NCBI ptt file), at 5,599,443 to 5,600,096
BT4248
BT4249: BT4249 - hypothetical protein (NCBI ptt file), at 5,600,363 to 5,600,596
BT4249
BT4250: BT4250 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 5,600,726 to 5,601,271
BT4250
BT4251: BT4251 - hypothetical protein (NCBI ptt file), at 5,601,336 to 5,603,183
BT4251
Position (kb)
5600
5601 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5599.466 kb on + strand at 5599.509 kb on - strand, within BT4248 at 5599.509 kb on - strand, within BT4248 at 5599.512 kb on + strand, within BT4248 at 5599.698 kb on + strand, within BT4248 at 5599.723 kb on + strand, within BT4248 at 5599.723 kb on + strand, within BT4248 at 5599.724 kb on - strand, within BT4248 at 5599.728 kb on + strand, within BT4248 at 5599.729 kb on - strand, within BT4248 at 5599.766 kb on + strand, within BT4248 at 5599.774 kb on - strand, within BT4248 at 5599.866 kb on - strand, within BT4248 at 5599.877 kb on + strand, within BT4248 at 5599.910 kb on + strand, within BT4248 at 5599.912 kb on + strand, within BT4248 at 5599.944 kb on + strand, within BT4248 at 5599.989 kb on - strand, within BT4248 at 5600.013 kb on - strand, within BT4248 at 5600.013 kb on - strand, within BT4248 at 5600.109 kb on + strand at 5600.170 kb on - strand at 5600.236 kb on - strand at 5600.322 kb on + strand at 5600.338 kb on + strand at 5600.355 kb on - strand at 5600.359 kb on + strand at 5600.360 kb on - strand at 5600.392 kb on + strand, within BT4249 at 5600.438 kb on + strand, within BT4249 at 5600.476 kb on - strand, within BT4249 at 5600.608 kb on - strand at 5600.652 kb on - strand at 5600.709 kb on - strand at 5600.755 kb on - strand at 5600.789 kb on + strand at 5600.790 kb on - strand, within BT4250 at 5600.814 kb on - strand, within BT4250 at 5600.832 kb on - strand, within BT4250 at 5600.834 kb on - strand, within BT4250 at 5600.863 kb on - strand, within BT4250 at 5600.947 kb on + strand, within BT4250 at 5600.953 kb on + strand, within BT4250 at 5600.956 kb on + strand, within BT4250 at 5600.960 kb on - strand, within BT4250 at 5600.962 kb on - strand, within BT4250 at 5600.962 kb on - strand, within BT4250 at 5601.042 kb on + strand, within BT4250 at 5601.043 kb on - strand, within BT4250 at 5601.077 kb on + strand, within BT4250 at 5601.077 kb on + strand, within BT4250 at 5601.077 kb on + strand, within BT4250 at 5601.077 kb on + strand, within BT4250 at 5601.078 kb on - strand, within BT4250 at 5601.150 kb on + strand, within BT4250 at 5601.151 kb on - strand, within BT4250 at 5601.155 kb on - strand, within BT4250 at 5601.216 kb on + strand, within BT4250 at 5601.270 kb on - strand at 5601.316 kb on - strand at 5601.324 kb on - strand at 5601.404 kb on + strand at 5601.411 kb on - strand at 5601.411 kb on - strand at 5601.411 kb on - strand at 5601.437 kb on + strand at 5601.437 kb on + strand at 5601.438 kb on - strand at 5601.449 kb on + strand at 5601.453 kb on + strand at 5601.461 kb on + strand at 5601.480 kb on - strand at 5601.480 kb on - strand at 5601.499 kb on + strand at 5601.522 kb on + strand, within BT4251 at 5601.589 kb on - strand, within BT4251
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 5,599,466 + +0.7 5,599,509 - BT4248 0.10 -1.0 5,599,509 - BT4248 0.10 -0.6 5,599,512 + BT4248 0.11 +1.3 5,599,698 + BT4248 0.39 +0.7 5,599,723 + BT4248 0.43 +1.0 5,599,723 + BT4248 0.43 -0.0 5,599,724 - BT4248 0.43 -2.1 5,599,728 + BT4248 0.44 -0.5 5,599,729 - BT4248 0.44 -4.5 5,599,766 + BT4248 0.49 +0.7 5,599,774 - BT4248 0.51 -1.6 5,599,866 - BT4248 0.65 +1.6 5,599,877 + BT4248 0.66 -1.9 5,599,910 + BT4248 0.71 -2.5 5,599,912 + BT4248 0.72 -1.2 5,599,944 + BT4248 0.77 -2.1 5,599,989 - BT4248 0.83 -0.4 5,600,013 - BT4248 0.87 -4.6 5,600,013 - BT4248 0.87 -2.4 5,600,109 + -2.2 5,600,170 - +1.6 5,600,236 - -3.1 5,600,322 + -1.7 5,600,338 + -1.4 5,600,355 - -1.7 5,600,359 + -0.4 5,600,360 - -1.6 5,600,392 + BT4249 0.12 +2.6 5,600,438 + BT4249 0.32 +1.3 5,600,476 - BT4249 0.48 +3.2 5,600,608 - -2.0 5,600,652 - -2.2 5,600,709 - +1.0 5,600,755 - -2.8 5,600,789 + +1.0 5,600,790 - BT4250 0.12 +0.1 5,600,814 - BT4250 0.16 -1.4 5,600,832 - BT4250 0.19 -3.2 5,600,834 - BT4250 0.20 +2.0 5,600,863 - BT4250 0.25 -2.4 5,600,947 + BT4250 0.40 +0.6 5,600,953 + BT4250 0.42 -0.4 5,600,956 + BT4250 0.42 -1.9 5,600,960 - BT4250 0.43 -1.6 5,600,962 - BT4250 0.43 -1.4 5,600,962 - BT4250 0.43 -0.4 5,601,042 + BT4250 0.58 -1.3 5,601,043 - BT4250 0.58 -2.8 5,601,077 + BT4250 0.64 -1.8 5,601,077 + BT4250 0.64 -2.3 5,601,077 + BT4250 0.64 -1.4 5,601,077 + BT4250 0.64 -1.2 5,601,078 - BT4250 0.64 -2.7 5,601,150 + BT4250 0.78 -0.7 5,601,151 - BT4250 0.78 +2.0 5,601,155 - BT4250 0.79 +3.3 5,601,216 + BT4250 0.90 -0.6 5,601,270 - -0.0 5,601,316 - -0.8 5,601,324 - -2.3 5,601,404 + +1.6 5,601,411 - +2.6 5,601,411 - -3.0 5,601,411 - -4.3 5,601,437 + -0.0 5,601,437 + -3.2 5,601,438 - -2.4 5,601,449 + +2.2 5,601,453 + -1.3 5,601,461 + +1.5 5,601,480 - -3.9 5,601,480 - +3.2 5,601,499 + -0.8 5,601,522 + BT4251 0.10 -2.5 5,601,589 - BT4251 0.14 -0.5
Or see this region's nucleotide sequence