Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4014 and BT4015 are separated by 281 nucleotides BT4015 and BT4016 are separated by 1 nucleotides BT4016 and BT4017 are separated by 5 nucleotides BT4017 and BT4018 overlap by 16 nucleotides
BT4014: BT4014 - putative type II restriction endonuclease (NCBI ptt file), at 5,232,104 to 5,233,120
BT4014
BT4015: BT4015 - transposase (NCBI ptt file), at 5,233,402 to 5,234,610
BT4015
BT4016: BT4016 - hypothetical protein (NCBI ptt file), at 5,234,612 to 5,235,034
BT4016
BT4017: BT4017 - hypothetical protein (NCBI ptt file), at 5,235,040 to 5,235,330
BT4017
BT4018: BT4018 - hypothetical protein (NCBI ptt file), at 5,235,315 to 5,235,782
BT4018
Position (kb)
5233
5234
5235 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5232.452 kb on + strand, within BT4014 at 5232.452 kb on + strand, within BT4014 at 5232.452 kb on + strand, within BT4014 at 5232.480 kb on + strand, within BT4014 at 5232.490 kb on + strand, within BT4014 at 5232.490 kb on + strand, within BT4014 at 5232.554 kb on + strand, within BT4014 at 5232.554 kb on + strand, within BT4014 at 5232.569 kb on + strand, within BT4014 at 5232.678 kb on + strand, within BT4014 at 5232.736 kb on + strand, within BT4014 at 5232.736 kb on + strand, within BT4014 at 5232.736 kb on + strand, within BT4014 at 5232.759 kb on + strand, within BT4014 at 5232.762 kb on + strand, within BT4014 at 5232.827 kb on + strand, within BT4014 at 5232.827 kb on + strand, within BT4014 at 5232.913 kb on + strand, within BT4014 at 5232.913 kb on + strand, within BT4014 at 5232.929 kb on + strand, within BT4014 at 5232.929 kb on + strand, within BT4014 at 5232.979 kb on + strand, within BT4014 at 5233.036 kb on + strand at 5233.036 kb on + strand at 5233.102 kb on + strand at 5233.123 kb on + strand at 5233.123 kb on + strand at 5233.156 kb on - strand at 5233.166 kb on - strand at 5233.202 kb on - strand at 5233.222 kb on + strand at 5233.223 kb on - strand at 5233.350 kb on + strand at 5233.353 kb on + strand at 5233.409 kb on - strand at 5233.447 kb on - strand at 5233.466 kb on + strand at 5233.467 kb on - strand at 5233.521 kb on + strand at 5233.530 kb on + strand, within BT4015 at 5233.572 kb on - strand, within BT4015 at 5233.700 kb on + strand, within BT4015 at 5233.700 kb on + strand, within BT4015 at 5233.701 kb on - strand, within BT4015 at 5233.721 kb on + strand, within BT4015 at 5233.743 kb on + strand, within BT4015 at 5233.832 kb on + strand, within BT4015 at 5233.833 kb on - strand, within BT4015 at 5233.863 kb on + strand, within BT4015 at 5233.895 kb on + strand, within BT4015 at 5233.944 kb on + strand, within BT4015 at 5234.048 kb on + strand, within BT4015 at 5234.049 kb on - strand, within BT4015 at 5234.065 kb on - strand, within BT4015 at 5234.072 kb on + strand, within BT4015 at 5234.072 kb on + strand, within BT4015 at 5234.080 kb on + strand, within BT4015 at 5234.080 kb on + strand, within BT4015 at 5234.114 kb on + strand, within BT4015 at 5234.178 kb on + strand, within BT4015 at 5234.178 kb on + strand, within BT4015 at 5234.271 kb on - strand, within BT4015 at 5234.317 kb on + strand, within BT4015 at 5234.318 kb on - strand, within BT4015 at 5234.320 kb on - strand, within BT4015 at 5234.395 kb on - strand, within BT4015 at 5234.417 kb on + strand, within BT4015 at 5234.443 kb on + strand, within BT4015 at 5234.444 kb on - strand, within BT4015 at 5234.445 kb on + strand, within BT4015 at 5234.445 kb on + strand, within BT4015 at 5234.446 kb on - strand, within BT4015 at 5234.453 kb on - strand, within BT4015 at 5234.549 kb on + strand at 5234.610 kb on + strand at 5234.712 kb on + strand, within BT4016 at 5234.713 kb on - strand, within BT4016 at 5234.776 kb on - strand, within BT4016 at 5234.829 kb on + strand, within BT4016 at 5234.830 kb on - strand, within BT4016 at 5234.912 kb on + strand, within BT4016 at 5234.913 kb on - strand, within BT4016 at 5234.913 kb on - strand, within BT4016 at 5234.913 kb on - strand, within BT4016 at 5234.928 kb on + strand, within BT4016 at 5235.019 kb on + strand at 5235.020 kb on - strand at 5235.065 kb on + strand at 5235.117 kb on + strand, within BT4017 at 5235.181 kb on + strand, within BT4017 at 5235.244 kb on + strand, within BT4017 at 5235.381 kb on + strand, within BT4018 at 5235.383 kb on + strand, within BT4018 at 5235.483 kb on - strand, within BT4018 at 5235.514 kb on + strand, within BT4018
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 5,232,452 + BT4014 0.34 +2.0 5,232,452 + BT4014 0.34 -3.2 5,232,452 + BT4014 0.34 +2.1 5,232,480 + BT4014 0.37 +0.1 5,232,490 + BT4014 0.38 +3.7 5,232,490 + BT4014 0.38 +0.1 5,232,554 + BT4014 0.44 -2.2 5,232,554 + BT4014 0.44 -2.2 5,232,569 + BT4014 0.46 +1.5 5,232,678 + BT4014 0.56 -0.9 5,232,736 + BT4014 0.62 -3.9 5,232,736 + BT4014 0.62 -3.0 5,232,736 + BT4014 0.62 -2.3 5,232,759 + BT4014 0.64 +0.1 5,232,762 + BT4014 0.65 -2.6 5,232,827 + BT4014 0.71 -2.5 5,232,827 + BT4014 0.71 +1.5 5,232,913 + BT4014 0.80 -2.9 5,232,913 + BT4014 0.80 -1.8 5,232,929 + BT4014 0.81 +1.7 5,232,929 + BT4014 0.81 +0.7 5,232,979 + BT4014 0.86 +1.8 5,233,036 + +1.7 5,233,036 + +2.7 5,233,102 + -1.7 5,233,123 + +1.7 5,233,123 + -1.9 5,233,156 - +0.1 5,233,166 - -1.9 5,233,202 - -1.8 5,233,222 + +0.1 5,233,223 - +1.1 5,233,350 + -4.9 5,233,353 + +2.8 5,233,409 - +0.7 5,233,447 - -2.8 5,233,466 + -0.9 5,233,467 - +2.7 5,233,521 + -2.1 5,233,530 + BT4015 0.11 -2.4 5,233,572 - BT4015 0.14 -1.2 5,233,700 + BT4015 0.25 +2.1 5,233,700 + BT4015 0.25 -3.0 5,233,701 - BT4015 0.25 -1.4 5,233,721 + BT4015 0.26 +2.3 5,233,743 + BT4015 0.28 -2.1 5,233,832 + BT4015 0.36 -1.1 5,233,833 - BT4015 0.36 -2.4 5,233,863 + BT4015 0.38 -3.2 5,233,895 + BT4015 0.41 +0.9 5,233,944 + BT4015 0.45 -2.9 5,234,048 + BT4015 0.53 -2.2 5,234,049 - BT4015 0.54 +0.1 5,234,065 - BT4015 0.55 -2.6 5,234,072 + BT4015 0.55 -3.1 5,234,072 + BT4015 0.55 -0.5 5,234,080 + BT4015 0.56 -1.2 5,234,080 + BT4015 0.56 +0.8 5,234,114 + BT4015 0.59 -2.5 5,234,178 + BT4015 0.64 -2.3 5,234,178 + BT4015 0.64 -1.6 5,234,271 - BT4015 0.72 -3.4 5,234,317 + BT4015 0.76 -2.6 5,234,318 - BT4015 0.76 -1.2 5,234,320 - BT4015 0.76 +0.7 5,234,395 - BT4015 0.82 -2.9 5,234,417 + BT4015 0.84 -1.5 5,234,443 + BT4015 0.86 -4.2 5,234,444 - BT4015 0.86 -2.7 5,234,445 + BT4015 0.86 -2.5 5,234,445 + BT4015 0.86 +1.2 5,234,446 - BT4015 0.86 -3.1 5,234,453 - BT4015 0.87 -2.4 5,234,549 + +1.3 5,234,610 + +1.1 5,234,712 + BT4016 0.24 -2.5 5,234,713 - BT4016 0.24 -1.4 5,234,776 - BT4016 0.39 -3.0 5,234,829 + BT4016 0.51 +0.1 5,234,830 - BT4016 0.52 +1.8 5,234,912 + BT4016 0.71 -1.2 5,234,913 - BT4016 0.71 +1.6 5,234,913 - BT4016 0.71 -3.0 5,234,913 - BT4016 0.71 -2.0 5,234,928 + BT4016 0.75 -1.1 5,235,019 + -1.2 5,235,020 - -1.7 5,235,065 + -3.4 5,235,117 + BT4017 0.26 -2.7 5,235,181 + BT4017 0.48 -0.6 5,235,244 + BT4017 0.70 +1.7 5,235,381 + BT4018 0.14 +1.3 5,235,383 + BT4018 0.15 -2.6 5,235,483 - BT4018 0.36 -0.7 5,235,514 + BT4018 0.43 -2.5
Or see this region's nucleotide sequence