Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4007 and BT4008 are separated by 63 nucleotides BT4008 and tRNA-Gln are separated by 264 nucleotides tRNA-Gln and BT4009 are separated by 169 nucleotides
BT4007: BT4007 - AAA-metalloprotease FtsH, with ATPase domain (NCBI ptt file), at 5,219,826 to 5,221,916
BT4007
BT4008: BT4008 - conserved hypothetical protein (NCBI ptt file), at 5,221,980 to 5,222,339
BT4008
BT_t65: tRNA-Gln - tRNA-Gln (RefSeq), at 5,222,604 to 5,222,677
tRNA-Gln
BT4009: BT4009 - integrase (NCBI ptt file), at 5,222,847 to 5,223,959
BT4009
Position (kb)
5221
5222
5223 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5222.039 kb on - strand, within BT4008 at 5222.077 kb on - strand, within BT4008 at 5222.077 kb on - strand, within BT4008 at 5222.077 kb on - strand, within BT4008 at 5222.184 kb on - strand, within BT4008 at 5222.218 kb on - strand, within BT4008 at 5222.218 kb on - strand, within BT4008 at 5222.248 kb on - strand, within BT4008 at 5222.250 kb on - strand, within BT4008 at 5222.307 kb on - strand at 5222.323 kb on - strand at 5222.346 kb on - strand at 5222.974 kb on + strand, within BT4009 at 5222.975 kb on - strand, within BT4009 at 5222.980 kb on + strand, within BT4009 at 5223.054 kb on + strand, within BT4009 at 5223.057 kb on - strand, within BT4009 at 5223.173 kb on + strand, within BT4009 at 5223.280 kb on + strand, within BT4009
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 5,222,039 - BT4008 0.16 -0.5 5,222,077 - BT4008 0.27 -0.9 5,222,077 - BT4008 0.27 +2.9 5,222,077 - BT4008 0.27 -3.8 5,222,184 - BT4008 0.57 -0.9 5,222,218 - BT4008 0.66 -5.0 5,222,218 - BT4008 0.66 -2.2 5,222,248 - BT4008 0.74 +2.7 5,222,250 - BT4008 0.75 -3.1 5,222,307 - -3.5 5,222,323 - +2.9 5,222,346 - -3.1 5,222,974 + BT4009 0.11 -2.9 5,222,975 - BT4009 0.12 -1.5 5,222,980 + BT4009 0.12 -0.5 5,223,054 + BT4009 0.19 -0.9 5,223,057 - BT4009 0.19 +1.0 5,223,173 + BT4009 0.29 +1.7 5,223,280 + BT4009 0.39 -1.0
Or see this region's nucleotide sequence