Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3906 and BT3907 overlap by 14 nucleotides BT3907 and BT3908 are separated by 18 nucleotides
BT3906: BT3906 - conserved hypothetical protein (NCBI ptt file), at 5,078,223 to 5,079,551
BT3906
BT3907: BT3907 - conserved hypothetical protein (NCBI ptt file), at 5,079,538 to 5,081,013
BT3907
BT3908: BT3908 - conserved hypothetical protein (NCBI ptt file), at 5,081,032 to 5,082,111
BT3908
Position (kb)
5079
5080
5081
5082 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5078.729 kb on + strand, within BT3906 at 5078.729 kb on + strand, within BT3906 at 5078.730 kb on - strand, within BT3906 at 5078.730 kb on - strand, within BT3906 at 5078.730 kb on - strand, within BT3906 at 5078.795 kb on - strand, within BT3906 at 5078.838 kb on + strand, within BT3906 at 5078.869 kb on - strand, within BT3906 at 5079.080 kb on + strand, within BT3906 at 5079.151 kb on - strand, within BT3906 at 5079.225 kb on - strand, within BT3906 at 5079.257 kb on - strand, within BT3906 at 5079.290 kb on - strand, within BT3906 at 5079.371 kb on - strand, within BT3906 at 5079.511 kb on - strand at 5079.545 kb on + strand at 5079.556 kb on - strand at 5079.680 kb on - strand at 5079.813 kb on + strand, within BT3907 at 5079.814 kb on - strand, within BT3907 at 5079.853 kb on + strand, within BT3907 at 5079.854 kb on - strand, within BT3907 at 5079.854 kb on - strand, within BT3907 at 5079.946 kb on + strand, within BT3907 at 5079.947 kb on - strand, within BT3907 at 5079.952 kb on + strand, within BT3907 at 5080.261 kb on - strand, within BT3907 at 5080.286 kb on + strand, within BT3907 at 5080.334 kb on + strand, within BT3907 at 5080.335 kb on - strand, within BT3907 at 5080.349 kb on + strand, within BT3907 at 5080.378 kb on + strand, within BT3907 at 5080.628 kb on - strand, within BT3907 at 5080.628 kb on - strand, within BT3907 at 5080.826 kb on - strand, within BT3907 at 5080.826 kb on - strand, within BT3907 at 5080.864 kb on + strand, within BT3907 at 5080.865 kb on - strand, within BT3907 at 5080.865 kb on - strand, within BT3907 at 5080.927 kb on - strand at 5080.929 kb on + strand at 5080.929 kb on + strand at 5080.932 kb on + strand at 5080.988 kb on + strand at 5081.151 kb on + strand, within BT3908 at 5081.200 kb on - strand, within BT3908 at 5081.203 kb on + strand, within BT3908 at 5081.204 kb on - strand, within BT3908 at 5081.212 kb on - strand, within BT3908 at 5081.265 kb on - strand, within BT3908 at 5081.267 kb on + strand, within BT3908 at 5081.314 kb on - strand at 5081.401 kb on + strand, within BT3908 at 5081.478 kb on + strand, within BT3908 at 5081.479 kb on - strand, within BT3908 at 5081.484 kb on - strand, within BT3908 at 5081.484 kb on - strand, within BT3908 at 5081.530 kb on - strand, within BT3908 at 5081.626 kb on - strand, within BT3908 at 5081.626 kb on - strand, within BT3908 at 5081.723 kb on + strand, within BT3908 at 5081.890 kb on - strand, within BT3908
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 5,078,729 + BT3906 0.38 +1.7 5,078,729 + BT3906 0.38 -2.4 5,078,730 - BT3906 0.38 -1.5 5,078,730 - BT3906 0.38 -2.7 5,078,730 - BT3906 0.38 +0.1 5,078,795 - BT3906 0.43 -0.9 5,078,838 + BT3906 0.46 -1.3 5,078,869 - BT3906 0.49 -2.7 5,079,080 + BT3906 0.64 +0.1 5,079,151 - BT3906 0.70 -0.2 5,079,225 - BT3906 0.75 -1.5 5,079,257 - BT3906 0.78 -0.9 5,079,290 - BT3906 0.80 -1.7 5,079,371 - BT3906 0.86 -1.7 5,079,511 - -3.0 5,079,545 + +0.1 5,079,556 - -1.9 5,079,680 - +2.1 5,079,813 + BT3907 0.19 +0.1 5,079,814 - BT3907 0.19 -1.6 5,079,853 + BT3907 0.21 -0.5 5,079,854 - BT3907 0.21 +0.1 5,079,854 - BT3907 0.21 +0.5 5,079,946 + BT3907 0.28 -4.0 5,079,947 - BT3907 0.28 +1.4 5,079,952 + BT3907 0.28 -1.9 5,080,261 - BT3907 0.49 -1.6 5,080,286 + BT3907 0.51 -1.9 5,080,334 + BT3907 0.54 -2.4 5,080,335 - BT3907 0.54 -2.5 5,080,349 + BT3907 0.55 -2.7 5,080,378 + BT3907 0.57 +0.5 5,080,628 - BT3907 0.74 +1.8 5,080,628 - BT3907 0.74 +2.8 5,080,826 - BT3907 0.87 +1.5 5,080,826 - BT3907 0.87 +1.1 5,080,864 + BT3907 0.90 -3.8 5,080,865 - BT3907 0.90 +1.1 5,080,865 - BT3907 0.90 -1.6 5,080,927 - +1.7 5,080,929 + -1.5 5,080,929 + -4.0 5,080,932 + +0.1 5,080,988 + -2.6 5,081,151 + BT3908 0.11 +0.1 5,081,200 - BT3908 0.16 +2.1 5,081,203 + BT3908 0.16 -2.1 5,081,204 - BT3908 0.16 -2.3 5,081,212 - BT3908 0.17 +1.3 5,081,265 - BT3908 0.22 +1.1 5,081,267 + BT3908 0.22 -1.9 5,081,314 - +0.1 5,081,401 + BT3908 0.34 -2.2 5,081,478 + BT3908 0.41 -2.1 5,081,479 - BT3908 0.41 -4.3 5,081,484 - BT3908 0.42 -2.5 5,081,484 - BT3908 0.42 +0.1 5,081,530 - BT3908 0.46 +2.1 5,081,626 - BT3908 0.55 -1.5 5,081,626 - BT3908 0.55 +1.8 5,081,723 + BT3908 0.64 -1.9 5,081,890 - BT3908 0.79 -2.7
Or see this region's nucleotide sequence