Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3746 and BT3747 are separated by 121 nucleotides BT3747 and BT3748 are separated by 324 nucleotides
BT3746: BT3746 - hypothetical protein (NCBI ptt file), at 4,865,027 to 4,865,440
BT3746
BT3747: BT3747 - hypothetical protein (NCBI ptt file), at 4,865,562 to 4,865,762
BT3747
BT3748: BT3748 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 4,866,087 to 4,866,629
BT3748
Position (kb)
4865
4866 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4864.220 kb on - strand at 4864.264 kb on - strand at 4864.298 kb on - strand at 4864.516 kb on + strand at 4864.526 kb on + strand at 4864.526 kb on + strand at 4864.551 kb on + strand at 4864.580 kb on + strand at 4864.609 kb on + strand at 4864.751 kb on + strand at 4864.752 kb on - strand at 4864.794 kb on - strand at 4864.794 kb on - strand at 4864.800 kb on + strand at 4864.804 kb on + strand at 4864.899 kb on - strand at 4864.910 kb on + strand at 4864.937 kb on + strand at 4864.937 kb on + strand at 4864.938 kb on - strand at 4864.941 kb on + strand at 4864.941 kb on + strand at 4864.941 kb on + strand at 4864.942 kb on - strand at 4864.942 kb on - strand at 4864.942 kb on - strand at 4864.960 kb on + strand at 4865.026 kb on + strand at 4865.114 kb on + strand, within BT3746 at 4865.114 kb on + strand, within BT3746 at 4865.158 kb on + strand, within BT3746 at 4865.158 kb on + strand, within BT3746 at 4865.159 kb on - strand, within BT3746 at 4865.159 kb on - strand, within BT3746 at 4865.189 kb on - strand, within BT3746 at 4865.189 kb on - strand, within BT3746 at 4865.193 kb on + strand, within BT3746 at 4865.193 kb on + strand, within BT3746 at 4865.215 kb on + strand, within BT3746 at 4865.216 kb on - strand, within BT3746 at 4865.229 kb on - strand, within BT3746 at 4865.309 kb on - strand, within BT3746 at 4865.324 kb on + strand, within BT3746 at 4865.338 kb on + strand, within BT3746 at 4865.358 kb on + strand, within BT3746 at 4865.439 kb on - strand at 4865.470 kb on + strand at 4865.471 kb on - strand at 4865.491 kb on + strand at 4865.492 kb on - strand at 4865.538 kb on - strand at 4865.557 kb on - strand at 4865.605 kb on + strand, within BT3747 at 4865.606 kb on - strand, within BT3747 at 4865.606 kb on - strand, within BT3747 at 4865.631 kb on + strand, within BT3747 at 4865.634 kb on - strand, within BT3747 at 4865.670 kb on - strand, within BT3747 at 4865.671 kb on + strand, within BT3747 at 4865.751 kb on - strand at 4865.757 kb on - strand at 4865.763 kb on - strand at 4865.779 kb on - strand at 4865.814 kb on - strand at 4865.839 kb on - strand at 4865.841 kb on + strand at 4865.841 kb on + strand at 4865.842 kb on - strand at 4865.842 kb on - strand at 4865.847 kb on - strand at 4865.847 kb on - strand at 4865.877 kb on - strand at 4865.929 kb on + strand at 4866.012 kb on - strand at 4866.045 kb on - strand at 4866.047 kb on - strand at 4866.047 kb on - strand at 4866.050 kb on + strand at 4866.051 kb on - strand at 4866.076 kb on + strand at 4866.076 kb on + strand at 4866.077 kb on - strand at 4866.114 kb on + strand at 4866.114 kb on + strand at 4866.116 kb on + strand at 4866.117 kb on - strand at 4866.117 kb on - strand at 4866.119 kb on + strand at 4866.120 kb on - strand at 4866.120 kb on - strand at 4866.124 kb on - strand at 4866.156 kb on + strand, within BT3748 at 4866.185 kb on + strand, within BT3748 at 4866.208 kb on - strand, within BT3748 at 4866.256 kb on + strand, within BT3748 at 4866.272 kb on - strand, within BT3748 at 4866.275 kb on + strand, within BT3748 at 4866.341 kb on + strand, within BT3748
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 4,864,220 - +1.1 4,864,264 - +3.1 4,864,298 - -2.9 4,864,516 + +1.7 4,864,526 + +1.1 4,864,526 + -1.5 4,864,551 + -1.5 4,864,580 + -1.3 4,864,609 + -2.3 4,864,751 + -2.6 4,864,752 - -2.7 4,864,794 - -1.9 4,864,794 - -0.9 4,864,800 + +0.1 4,864,804 + +1.7 4,864,899 - -1.5 4,864,910 + -2.5 4,864,937 + +0.7 4,864,937 + -3.0 4,864,938 - -2.4 4,864,941 + -1.9 4,864,941 + -0.1 4,864,941 + -2.4 4,864,942 - +0.8 4,864,942 - -1.6 4,864,942 - +2.4 4,864,960 + -1.4 4,865,026 + -1.8 4,865,114 + BT3746 0.21 +1.4 4,865,114 + BT3746 0.21 -2.7 4,865,158 + BT3746 0.32 -1.9 4,865,158 + BT3746 0.32 +2.6 4,865,159 - BT3746 0.32 -1.6 4,865,159 - BT3746 0.32 +1.7 4,865,189 - BT3746 0.39 -1.2 4,865,189 - BT3746 0.39 +1.4 4,865,193 + BT3746 0.40 -3.5 4,865,193 + BT3746 0.40 -1.3 4,865,215 + BT3746 0.45 +1.9 4,865,216 - BT3746 0.46 +0.7 4,865,229 - BT3746 0.49 +1.1 4,865,309 - BT3746 0.68 +1.8 4,865,324 + BT3746 0.72 -1.5 4,865,338 + BT3746 0.75 -2.0 4,865,358 + BT3746 0.80 -2.3 4,865,439 - +0.7 4,865,470 + -1.5 4,865,471 - +0.6 4,865,491 + +0.1 4,865,492 - +1.4 4,865,538 - +1.1 4,865,557 - -2.5 4,865,605 + BT3747 0.21 +2.5 4,865,606 - BT3747 0.22 -2.2 4,865,606 - BT3747 0.22 -2.5 4,865,631 + BT3747 0.34 -0.4 4,865,634 - BT3747 0.36 -1.5 4,865,670 - BT3747 0.54 +0.1 4,865,671 + BT3747 0.54 -1.1 4,865,751 - -0.1 4,865,757 - -2.4 4,865,763 - +2.9 4,865,779 - +2.1 4,865,814 - -4.7 4,865,839 - +0.7 4,865,841 + -1.9 4,865,841 + -2.5 4,865,842 - +0.1 4,865,842 - +2.2 4,865,847 - -1.7 4,865,847 - +2.3 4,865,877 - -4.3 4,865,929 + -3.0 4,866,012 - -2.0 4,866,045 - -2.5 4,866,047 - -2.1 4,866,047 - -2.3 4,866,050 + +0.1 4,866,051 - -1.6 4,866,076 + -1.3 4,866,076 + -1.9 4,866,077 - -2.6 4,866,114 + -1.3 4,866,114 + +1.7 4,866,116 + -2.5 4,866,117 - +0.5 4,866,117 - -4.1 4,866,119 + -4.8 4,866,120 - -0.9 4,866,120 - -2.7 4,866,124 - -2.8 4,866,156 + BT3748 0.13 -1.8 4,866,185 + BT3748 0.18 +2.1 4,866,208 - BT3748 0.22 +0.1 4,866,256 + BT3748 0.31 +3.7 4,866,272 - BT3748 0.34 -2.9 4,866,275 + BT3748 0.35 +3.4 4,866,341 + BT3748 0.47 -3.0
Or see this region's nucleotide sequence