Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3614 and BT3615 are separated by 15 nucleotides BT3615 and BT3616 are separated by 41 nucleotides BT3616 and BT3617 are separated by 23 nucleotides
BT3614: BT3614 - putative oxidoreductase (NCBI ptt file), at 4,683,105 to 4,684,037
BT3614
BT3615: BT3615 - conserved hypothetical protein (NCBI ptt file), at 4,684,053 to 4,684,976
BT3615
BT3616: BT3616 - fucose permease (NCBI ptt file), at 4,685,018 to 4,686,274
BT3616
BT3617: BT3617 - sorbitol dehydrogenase (NCBI ptt file), at 4,686,298 to 4,687,314
BT3617
Position (kb)
4685
4686
4687 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4684.018 kb on + strand at 4684.023 kb on + strand at 4684.061 kb on + strand at 4684.278 kb on - strand, within BT3615 at 4684.441 kb on + strand, within BT3615 at 4684.578 kb on - strand, within BT3615 at 4684.745 kb on - strand, within BT3615 at 4684.778 kb on + strand, within BT3615 at 4684.833 kb on + strand, within BT3615 at 4684.834 kb on - strand, within BT3615 at 4684.834 kb on - strand, within BT3615 at 4684.834 kb on - strand, within BT3615 at 4684.954 kb on - strand at 4684.964 kb on + strand at 4684.989 kb on + strand at 4684.994 kb on + strand at 4685.014 kb on - strand at 4685.014 kb on - strand at 4685.033 kb on + strand at 4685.033 kb on + strand at 4685.033 kb on + strand at 4685.033 kb on + strand at 4685.033 kb on + strand at 4685.034 kb on - strand at 4685.036 kb on - strand at 4685.036 kb on - strand at 4685.183 kb on + strand, within BT3616 at 4685.188 kb on + strand, within BT3616 at 4685.209 kb on + strand, within BT3616 at 4685.209 kb on + strand, within BT3616 at 4685.210 kb on - strand, within BT3616 at 4685.226 kb on - strand, within BT3616 at 4685.231 kb on + strand, within BT3616 at 4685.232 kb on - strand, within BT3616 at 4685.232 kb on - strand, within BT3616 at 4685.342 kb on - strand, within BT3616 at 4685.384 kb on + strand, within BT3616 at 4685.385 kb on - strand, within BT3616 at 4685.388 kb on + strand, within BT3616 at 4685.512 kb on + strand, within BT3616 at 4685.513 kb on - strand, within BT3616 at 4685.513 kb on - strand, within BT3616 at 4685.560 kb on + strand, within BT3616 at 4685.739 kb on + strand, within BT3616 at 4685.740 kb on - strand, within BT3616 at 4685.768 kb on + strand, within BT3616 at 4685.771 kb on - strand, within BT3616 at 4685.771 kb on - strand, within BT3616 at 4685.788 kb on + strand, within BT3616 at 4685.808 kb on + strand, within BT3616 at 4685.811 kb on - strand, within BT3616 at 4685.823 kb on - strand, within BT3616 at 4685.867 kb on + strand, within BT3616 at 4685.868 kb on - strand, within BT3616 at 4685.868 kb on - strand, within BT3616 at 4685.869 kb on + strand, within BT3616 at 4685.869 kb on + strand, within BT3616 at 4685.870 kb on - strand, within BT3616 at 4685.935 kb on + strand, within BT3616 at 4685.936 kb on - strand, within BT3616 at 4685.936 kb on - strand, within BT3616 at 4686.011 kb on + strand, within BT3616 at 4686.038 kb on - strand, within BT3616 at 4686.164 kb on + strand at 4686.243 kb on - strand at 4686.265 kb on - strand at 4686.377 kb on + strand at 4686.383 kb on + strand at 4686.557 kb on - strand, within BT3617 at 4686.559 kb on - strand, within BT3617 at 4686.615 kb on + strand, within BT3617 at 4686.633 kb on - strand, within BT3617 at 4686.659 kb on - strand, within BT3617 at 4686.697 kb on - strand, within BT3617 at 4686.699 kb on + strand, within BT3617 at 4686.700 kb on - strand, within BT3617 at 4686.700 kb on - strand, within BT3617 at 4686.700 kb on - strand, within BT3617 at 4686.830 kb on + strand, within BT3617 at 4686.831 kb on - strand, within BT3617 at 4686.891 kb on - strand, within BT3617 at 4687.021 kb on + strand, within BT3617 at 4687.021 kb on + strand, within BT3617 at 4687.022 kb on - strand, within BT3617 at 4687.025 kb on + strand, within BT3617 at 4687.028 kb on - strand, within BT3617 at 4687.068 kb on + strand, within BT3617 at 4687.183 kb on + strand, within BT3617
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 4,684,018 + -0.5 4,684,023 + +1.7 4,684,061 + -2.5 4,684,278 - BT3615 0.24 -0.7 4,684,441 + BT3615 0.42 -2.0 4,684,578 - BT3615 0.57 +1.5 4,684,745 - BT3615 0.75 -1.6 4,684,778 + BT3615 0.78 +1.1 4,684,833 + BT3615 0.84 -1.1 4,684,834 - BT3615 0.85 -2.5 4,684,834 - BT3615 0.85 -1.6 4,684,834 - BT3615 0.85 -3.3 4,684,954 - -2.7 4,684,964 + -3.8 4,684,989 + -2.9 4,684,994 + -1.6 4,685,014 - -2.7 4,685,014 - -1.5 4,685,033 + -1.5 4,685,033 + -2.7 4,685,033 + -1.7 4,685,033 + +1.9 4,685,033 + -1.9 4,685,034 - -3.2 4,685,036 - -1.1 4,685,036 - +2.4 4,685,183 + BT3616 0.13 +0.3 4,685,188 + BT3616 0.14 -3.4 4,685,209 + BT3616 0.15 +0.1 4,685,209 + BT3616 0.15 +0.1 4,685,210 - BT3616 0.15 -2.5 4,685,226 - BT3616 0.17 -0.9 4,685,231 + BT3616 0.17 -2.9 4,685,232 - BT3616 0.17 -3.1 4,685,232 - BT3616 0.17 -2.4 4,685,342 - BT3616 0.26 -3.6 4,685,384 + BT3616 0.29 -2.2 4,685,385 - BT3616 0.29 -1.7 4,685,388 + BT3616 0.29 -3.7 4,685,512 + BT3616 0.39 +2.9 4,685,513 - BT3616 0.39 -4.8 4,685,513 - BT3616 0.39 +0.7 4,685,560 + BT3616 0.43 -2.7 4,685,739 + BT3616 0.57 +0.1 4,685,740 - BT3616 0.57 -1.5 4,685,768 + BT3616 0.60 -4.1 4,685,771 - BT3616 0.60 +1.3 4,685,771 - BT3616 0.60 -3.1 4,685,788 + BT3616 0.61 +1.8 4,685,808 + BT3616 0.63 -2.6 4,685,811 - BT3616 0.63 +2.4 4,685,823 - BT3616 0.64 -2.2 4,685,867 + BT3616 0.68 -2.1 4,685,868 - BT3616 0.68 -3.8 4,685,868 - BT3616 0.68 -3.5 4,685,869 + BT3616 0.68 +0.1 4,685,869 + BT3616 0.68 -3.4 4,685,870 - BT3616 0.68 -2.4 4,685,935 + BT3616 0.73 -2.2 4,685,936 - BT3616 0.73 -1.7 4,685,936 - BT3616 0.73 -2.0 4,686,011 + BT3616 0.79 -2.1 4,686,038 - BT3616 0.81 -2.3 4,686,164 + -1.0 4,686,243 - -0.3 4,686,265 - -3.5 4,686,377 + +0.1 4,686,383 + -2.4 4,686,557 - BT3617 0.25 -3.4 4,686,559 - BT3617 0.26 -2.7 4,686,615 + BT3617 0.31 -2.2 4,686,633 - BT3617 0.33 -2.3 4,686,659 - BT3617 0.35 -1.5 4,686,697 - BT3617 0.39 +1.8 4,686,699 + BT3617 0.39 +0.3 4,686,700 - BT3617 0.40 -3.1 4,686,700 - BT3617 0.40 +0.5 4,686,700 - BT3617 0.40 +2.1 4,686,830 + BT3617 0.52 -1.2 4,686,831 - BT3617 0.52 -3.8 4,686,891 - BT3617 0.58 +3.2 4,687,021 + BT3617 0.71 -2.6 4,687,021 + BT3617 0.71 -0.5 4,687,022 - BT3617 0.71 -2.6 4,687,025 + BT3617 0.71 -2.5 4,687,028 - BT3617 0.72 +0.1 4,687,068 + BT3617 0.76 -1.8 4,687,183 + BT3617 0.87 +1.3
Or see this region's nucleotide sequence