Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3245 and BT3246 are separated by 75 nucleotides BT3246 and BT3247 are separated by 102 nucleotides BT3247 and BT3248 are separated by 418 nucleotides
BT3245: BT3245 - putative iron-sulfur cluster-binding protein (NCBI ptt file), at 4,142,526 to 4,143,557
BT3245
BT3246: BT3246 - polysaccharide deacetylase (NCBI ptt file), at 4,143,633 to 4,144,247
BT3246
BT3247: BT3247 - conserved hypothetical protein (NCBI ptt file), at 4,144,350 to 4,147,724
BT3247
BT3248: BT3248 - putative metal ABC transporter, ATP-binding protein (NCBI ptt file), at 4,148,143 to 4,148,964
BT3248
Position (kb)
4144
4145
4146
4147
4148 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4143.383 kb on + strand, within BT3245 at 4143.392 kb on - strand, within BT3245 at 4143.403 kb on + strand at 4143.404 kb on - strand, within BT3245 at 4143.612 kb on - strand at 4143.635 kb on - strand at 4143.635 kb on - strand at 4144.258 kb on - strand at 4144.258 kb on - strand at 4144.275 kb on - strand at 4144.278 kb on - strand at 4144.292 kb on - strand at 4144.320 kb on - strand at 4144.320 kb on - strand at 4144.324 kb on - strand at 4144.331 kb on - strand at 4144.334 kb on - strand at 4144.341 kb on - strand at 4144.385 kb on - strand at 4144.453 kb on - strand at 4144.453 kb on - strand at 4144.453 kb on - strand at 4144.481 kb on - strand at 4144.544 kb on - strand at 4144.613 kb on - strand at 4144.657 kb on - strand at 4144.772 kb on - strand, within BT3247 at 4144.802 kb on - strand, within BT3247 at 4144.856 kb on - strand, within BT3247 at 4145.190 kb on - strand, within BT3247 at 4145.255 kb on - strand, within BT3247 at 4145.336 kb on - strand, within BT3247 at 4145.505 kb on - strand, within BT3247 at 4145.559 kb on - strand, within BT3247 at 4145.778 kb on - strand, within BT3247 at 4145.816 kb on - strand, within BT3247 at 4146.007 kb on - strand, within BT3247 at 4146.112 kb on - strand, within BT3247 at 4146.176 kb on - strand, within BT3247 at 4146.194 kb on - strand, within BT3247 at 4146.329 kb on - strand, within BT3247 at 4146.365 kb on - strand, within BT3247 at 4146.392 kb on - strand, within BT3247 at 4146.638 kb on - strand, within BT3247 at 4147.760 kb on - strand at 4147.836 kb on + strand at 4147.857 kb on - strand at 4147.883 kb on + strand at 4147.895 kb on + strand at 4147.896 kb on - strand at 4147.900 kb on - strand at 4147.902 kb on - strand at 4147.902 kb on - strand at 4147.902 kb on - strand at 4147.910 kb on + strand at 4147.925 kb on + strand at 4147.925 kb on + strand at 4147.926 kb on - strand at 4147.927 kb on + strand at 4147.927 kb on + strand at 4147.928 kb on - strand at 4147.978 kb on + strand at 4147.982 kb on - strand at 4148.020 kb on - strand at 4148.088 kb on - strand at 4148.098 kb on + strand at 4148.098 kb on + strand at 4148.098 kb on + strand at 4148.098 kb on + strand at 4148.098 kb on + strand at 4148.123 kb on + strand at 4148.144 kb on + strand at 4148.264 kb on + strand, within BT3248 at 4148.311 kb on + strand, within BT3248 at 4148.312 kb on - strand, within BT3248 at 4148.312 kb on - strand, within BT3248 at 4148.379 kb on - strand, within BT3248 at 4148.408 kb on + strand, within BT3248 at 4148.479 kb on + strand, within BT3248 at 4148.494 kb on - strand, within BT3248 at 4148.494 kb on - strand, within BT3248 at 4148.547 kb on + strand, within BT3248 at 4148.547 kb on + strand, within BT3248 at 4148.548 kb on - strand, within BT3248 at 4148.548 kb on - strand, within BT3248 at 4148.665 kb on - strand, within BT3248 at 4148.666 kb on + strand, within BT3248 at 4148.667 kb on - strand, within BT3248 at 4148.679 kb on - strand, within BT3248
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 4,143,383 + BT3245 0.83 -4.2 4,143,392 - BT3245 0.84 -2.7 4,143,403 + -1.1 4,143,404 - BT3245 0.85 -3.9 4,143,612 - -3.2 4,143,635 - -3.3 4,143,635 - +0.4 4,144,258 - -2.4 4,144,258 - -3.0 4,144,275 - -2.7 4,144,278 - -2.9 4,144,292 - +2.8 4,144,320 - -1.9 4,144,320 - -3.1 4,144,324 - -2.2 4,144,331 - +1.6 4,144,334 - -1.6 4,144,341 - +2.5 4,144,385 - +0.7 4,144,453 - -3.9 4,144,453 - -3.6 4,144,453 - -4.0 4,144,481 - -2.2 4,144,544 - -4.8 4,144,613 - -2.9 4,144,657 - -0.9 4,144,772 - BT3247 0.13 -1.9 4,144,802 - BT3247 0.13 -1.9 4,144,856 - BT3247 0.15 -1.5 4,145,190 - BT3247 0.25 -3.1 4,145,255 - BT3247 0.27 -4.1 4,145,336 - BT3247 0.29 -1.5 4,145,505 - BT3247 0.34 -3.5 4,145,559 - BT3247 0.36 -3.6 4,145,778 - BT3247 0.42 -1.5 4,145,816 - BT3247 0.43 +0.1 4,146,007 - BT3247 0.49 -2.8 4,146,112 - BT3247 0.52 -2.5 4,146,176 - BT3247 0.54 -2.9 4,146,194 - BT3247 0.55 -3.0 4,146,329 - BT3247 0.59 -3.0 4,146,365 - BT3247 0.60 -4.1 4,146,392 - BT3247 0.61 -3.6 4,146,638 - BT3247 0.68 -3.6 4,147,760 - -2.3 4,147,836 + -0.9 4,147,857 - +0.1 4,147,883 + -2.8 4,147,895 + -2.8 4,147,896 - -3.2 4,147,900 - -1.8 4,147,902 - +1.4 4,147,902 - -2.4 4,147,902 - -1.3 4,147,910 + +1.6 4,147,925 + -1.3 4,147,925 + -3.9 4,147,926 - -2.2 4,147,927 + -2.2 4,147,927 + -2.6 4,147,928 - -1.8 4,147,978 + -2.6 4,147,982 - -3.7 4,148,020 - -2.0 4,148,088 - -2.2 4,148,098 + -1.0 4,148,098 + -1.7 4,148,098 + -0.2 4,148,098 + -1.1 4,148,098 + +2.0 4,148,123 + -0.9 4,148,144 + -2.5 4,148,264 + BT3248 0.15 -0.9 4,148,311 + BT3248 0.20 -0.2 4,148,312 - BT3248 0.21 +1.3 4,148,312 - BT3248 0.21 -2.9 4,148,379 - BT3248 0.29 +0.1 4,148,408 + BT3248 0.32 -2.5 4,148,479 + BT3248 0.41 -0.9 4,148,494 - BT3248 0.43 -3.5 4,148,494 - BT3248 0.43 -2.5 4,148,547 + BT3248 0.49 +0.8 4,148,547 + BT3248 0.49 -2.1 4,148,548 - BT3248 0.49 -3.3 4,148,548 - BT3248 0.49 +0.4 4,148,665 - BT3248 0.64 -2.7 4,148,666 + BT3248 0.64 -2.9 4,148,667 - BT3248 0.64 -3.2 4,148,679 - BT3248 0.65 -3.5
Or see this region's nucleotide sequence