Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2690 and BT2691 overlap by 4 nucleotides BT2691 and BT2692 are separated by 21 nucleotides BT2692 and BT2693 are separated by 97 nucleotides
BT2690: BT2690 - histidine ammonia-lyase (NCBI ptt file), at 3,352,437 to 3,353,936
BT2690
BT2691: BT2691 - methenyltetrahydrofolate cyclohydrolase (NCBI ptt file), at 3,353,933 to 3,354,562
BT2691
BT2692: BT2692 - imidazolonepropionase (NCBI ptt file), at 3,354,584 to 3,355,837
BT2692
BT2693: BT2693 - formiminotransferase-cyclodeaminase (NCBI ptt file), at 3,355,935 to 3,356,837
BT2693
Position (kb)
3354
3355
3356 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3353.726 kb on + strand, within BT2690 at 3353.727 kb on - strand, within BT2690 at 3353.913 kb on + strand at 3353.913 kb on + strand at 3353.914 kb on - strand at 3353.936 kb on + strand at 3353.966 kb on + strand at 3353.967 kb on - strand at 3354.046 kb on + strand, within BT2691 at 3354.061 kb on + strand, within BT2691 at 3354.167 kb on + strand, within BT2691 at 3354.331 kb on + strand, within BT2691 at 3354.526 kb on + strand at 3354.526 kb on + strand at 3354.527 kb on - strand at 3354.543 kb on - strand at 3354.557 kb on + strand at 3354.566 kb on - strand at 3354.578 kb on + strand at 3354.599 kb on + strand at 3354.599 kb on + strand at 3354.600 kb on - strand at 3354.613 kb on + strand at 3354.614 kb on - strand at 3354.645 kb on + strand at 3354.646 kb on - strand at 3354.646 kb on - strand at 3354.787 kb on + strand, within BT2692 at 3354.798 kb on + strand, within BT2692 at 3354.798 kb on + strand, within BT2692 at 3354.802 kb on + strand, within BT2692 at 3354.915 kb on + strand, within BT2692 at 3354.956 kb on - strand at 3354.959 kb on + strand, within BT2692 at 3354.963 kb on - strand, within BT2692 at 3355.239 kb on + strand, within BT2692 at 3355.240 kb on - strand, within BT2692 at 3355.258 kb on + strand, within BT2692 at 3355.287 kb on - strand, within BT2692 at 3355.299 kb on - strand, within BT2692 at 3355.299 kb on - strand, within BT2692 at 3355.309 kb on + strand, within BT2692 at 3355.309 kb on + strand, within BT2692 at 3355.401 kb on - strand, within BT2692 at 3355.417 kb on + strand, within BT2692 at 3355.490 kb on + strand, within BT2692 at 3355.532 kb on - strand, within BT2692 at 3355.534 kb on - strand, within BT2692 at 3355.645 kb on - strand, within BT2692 at 3355.663 kb on - strand, within BT2692 at 3355.701 kb on + strand, within BT2692 at 3355.702 kb on - strand, within BT2692 at 3355.728 kb on - strand at 3355.743 kb on - strand at 3355.818 kb on - strand at 3355.819 kb on + strand at 3355.820 kb on - strand at 3355.839 kb on + strand at 3355.860 kb on + strand at 3355.916 kb on + strand at 3355.921 kb on + strand at 3355.922 kb on - strand at 3355.996 kb on + strand at 3356.010 kb on + strand at 3356.108 kb on - strand, within BT2693 at 3356.145 kb on + strand, within BT2693 at 3356.145 kb on + strand, within BT2693 at 3356.157 kb on - strand, within BT2693 at 3356.213 kb on + strand, within BT2693 at 3356.277 kb on - strand, within BT2693 at 3356.306 kb on + strand, within BT2693 at 3356.310 kb on + strand, within BT2693 at 3356.474 kb on + strand, within BT2693 at 3356.475 kb on - strand, within BT2693 at 3356.476 kb on + strand, within BT2693 at 3356.476 kb on + strand, within BT2693 at 3356.476 kb on + strand, within BT2693 at 3356.477 kb on - strand, within BT2693 at 3356.537 kb on + strand, within BT2693 at 3356.537 kb on + strand, within BT2693 at 3356.606 kb on + strand, within BT2693 at 3356.607 kb on - strand, within BT2693 at 3356.607 kb on - strand, within BT2693 at 3356.763 kb on + strand at 3356.763 kb on + strand at 3356.825 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 3,353,726 + BT2690 0.86 -1.8 3,353,727 - BT2690 0.86 -2.0 3,353,913 + -2.7 3,353,913 + -1.8 3,353,914 - -2.2 3,353,936 + +0.9 3,353,966 + -1.5 3,353,967 - -3.6 3,354,046 + BT2691 0.18 +0.5 3,354,061 + BT2691 0.20 -3.0 3,354,167 + BT2691 0.37 -4.3 3,354,331 + BT2691 0.63 -1.7 3,354,526 + -1.5 3,354,526 + -1.9 3,354,527 - -3.6 3,354,543 - -2.8 3,354,557 + +1.5 3,354,566 - -1.5 3,354,578 + -2.3 3,354,599 + -2.5 3,354,599 + -1.7 3,354,600 - -0.9 3,354,613 + +0.9 3,354,614 - -3.3 3,354,645 + +2.4 3,354,646 - -2.6 3,354,646 - -2.6 3,354,787 + BT2692 0.16 -2.7 3,354,798 + BT2692 0.17 +1.4 3,354,798 + BT2692 0.17 +0.1 3,354,802 + BT2692 0.17 -0.9 3,354,915 + BT2692 0.26 +2.1 3,354,956 - -1.9 3,354,959 + BT2692 0.30 -2.8 3,354,963 - BT2692 0.30 -3.3 3,355,239 + BT2692 0.52 -3.5 3,355,240 - BT2692 0.52 +0.1 3,355,258 + BT2692 0.54 -3.1 3,355,287 - BT2692 0.56 -1.5 3,355,299 - BT2692 0.57 -1.7 3,355,299 - BT2692 0.57 -0.9 3,355,309 + BT2692 0.58 +2.6 3,355,309 + BT2692 0.58 -0.5 3,355,401 - BT2692 0.65 +1.9 3,355,417 + BT2692 0.66 -0.9 3,355,490 + BT2692 0.72 -1.2 3,355,532 - BT2692 0.76 -2.2 3,355,534 - BT2692 0.76 -1.5 3,355,645 - BT2692 0.85 -2.5 3,355,663 - BT2692 0.86 +2.7 3,355,701 + BT2692 0.89 -2.2 3,355,702 - BT2692 0.89 -2.0 3,355,728 - +1.9 3,355,743 - +0.1 3,355,818 - -3.3 3,355,819 + +0.9 3,355,820 - -1.6 3,355,839 + -2.5 3,355,860 + -0.9 3,355,916 + +0.1 3,355,921 + -1.9 3,355,922 - +0.9 3,355,996 + -1.7 3,356,010 + -2.5 3,356,108 - BT2693 0.19 -2.7 3,356,145 + BT2693 0.23 -3.6 3,356,145 + BT2693 0.23 -2.7 3,356,157 - BT2693 0.25 +0.1 3,356,213 + BT2693 0.31 -2.8 3,356,277 - BT2693 0.38 -2.1 3,356,306 + BT2693 0.41 +3.3 3,356,310 + BT2693 0.42 +1.1 3,356,474 + BT2693 0.60 +3.6 3,356,475 - BT2693 0.60 -2.2 3,356,476 + BT2693 0.60 -2.1 3,356,476 + BT2693 0.60 -2.8 3,356,476 + BT2693 0.60 -2.4 3,356,477 - BT2693 0.60 -0.9 3,356,537 + BT2693 0.67 -2.5 3,356,537 + BT2693 0.67 +0.5 3,356,606 + BT2693 0.74 +0.4 3,356,607 - BT2693 0.74 +0.9 3,356,607 - BT2693 0.74 -2.5 3,356,763 + -2.3 3,356,763 + +1.7 3,356,825 + +1.5
Or see this region's nucleotide sequence