Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2304 and BT2305 are separated by 152 nucleotides BT2305 and BT2306 are separated by 49 nucleotides
BT2304: BT2304 - hypothetical protein (NCBI ptt file), at 2,878,549 to 2,879,244
BT2304
BT2305: BT2305 - putative mobilization protein (NCBI ptt file), at 2,879,397 to 2,880,623
BT2305
BT2306: BT2306 - putative mobilization protein (NCBI ptt file), at 2,880,673 to 2,882,682
BT2306
Position (kb)
2879
2880
2881 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2878.520 kb on - strand at 2878.688 kb on - strand, within BT2304 at 2878.688 kb on - strand, within BT2304 at 2878.689 kb on + strand, within BT2304 at 2878.817 kb on + strand, within BT2304 at 2878.818 kb on - strand, within BT2304 at 2878.956 kb on - strand, within BT2304 at 2878.962 kb on + strand, within BT2304 at 2878.971 kb on + strand, within BT2304 at 2878.971 kb on + strand, within BT2304 at 2878.974 kb on - strand, within BT2304 at 2879.206 kb on - strand at 2879.319 kb on - strand at 2879.445 kb on + strand at 2879.445 kb on + strand at 2879.446 kb on - strand at 2879.596 kb on + strand, within BT2305 at 2879.597 kb on - strand, within BT2305 at 2879.597 kb on - strand, within BT2305 at 2879.965 kb on - strand, within BT2305 at 2880.010 kb on - strand, within BT2305 at 2880.011 kb on + strand, within BT2305 at 2880.016 kb on + strand, within BT2305 at 2880.057 kb on + strand, within BT2305 at 2880.067 kb on + strand, within BT2305 at 2880.068 kb on - strand, within BT2305 at 2880.068 kb on - strand, within BT2305 at 2880.349 kb on + strand, within BT2305 at 2880.371 kb on - strand, within BT2305 at 2880.371 kb on - strand, within BT2305 at 2880.374 kb on + strand, within BT2305 at 2880.637 kb on - strand at 2880.637 kb on - strand at 2880.670 kb on - strand at 2880.672 kb on + strand at 2880.720 kb on + strand at 2880.737 kb on + strand at 2880.740 kb on + strand at 2880.757 kb on - strand at 2880.927 kb on - strand, within BT2306 at 2880.927 kb on - strand, within BT2306 at 2881.050 kb on + strand, within BT2306 at 2881.051 kb on - strand, within BT2306 at 2881.053 kb on - strand, within BT2306 at 2881.222 kb on + strand, within BT2306 at 2881.223 kb on - strand, within BT2306 at 2881.369 kb on + strand, within BT2306 at 2881.369 kb on + strand, within BT2306 at 2881.411 kb on + strand, within BT2306 at 2881.412 kb on - strand, within BT2306 at 2881.412 kb on - strand, within BT2306 at 2881.412 kb on - strand, within BT2306 at 2881.441 kb on + strand, within BT2306 at 2881.444 kb on - strand, within BT2306 at 2881.463 kb on - strand, within BT2306 at 2881.469 kb on + strand, within BT2306 at 2881.547 kb on - strand, within BT2306
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 2,878,520 - -1.0 2,878,688 - BT2304 0.20 -0.5 2,878,688 - BT2304 0.20 -2.0 2,878,689 + BT2304 0.20 -1.0 2,878,817 + BT2304 0.39 -0.5 2,878,818 - BT2304 0.39 -3.3 2,878,956 - BT2304 0.58 -1.8 2,878,962 + BT2304 0.59 -3.9 2,878,971 + BT2304 0.61 +0.6 2,878,971 + BT2304 0.61 -2.3 2,878,974 - BT2304 0.61 -2.4 2,879,206 - -3.3 2,879,319 - -1.8 2,879,445 + +1.9 2,879,445 + +0.0 2,879,446 - +0.4 2,879,596 + BT2305 0.16 -1.2 2,879,597 - BT2305 0.16 -2.5 2,879,597 - BT2305 0.16 +2.4 2,879,965 - BT2305 0.46 -2.1 2,880,010 - BT2305 0.50 +1.9 2,880,011 + BT2305 0.50 -3.5 2,880,016 + BT2305 0.50 -3.7 2,880,057 + BT2305 0.54 -2.3 2,880,067 + BT2305 0.55 -2.2 2,880,068 - BT2305 0.55 -1.5 2,880,068 - BT2305 0.55 -3.0 2,880,349 + BT2305 0.78 -4.9 2,880,371 - BT2305 0.79 -4.5 2,880,371 - BT2305 0.79 -3.8 2,880,374 + BT2305 0.80 -3.8 2,880,637 - -3.0 2,880,637 - +2.3 2,880,670 - -0.7 2,880,672 + -2.0 2,880,720 + -2.0 2,880,737 + -1.3 2,880,740 + -2.7 2,880,757 - -1.7 2,880,927 - BT2306 0.13 -3.0 2,880,927 - BT2306 0.13 -2.9 2,881,050 + BT2306 0.19 +2.0 2,881,051 - BT2306 0.19 -1.6 2,881,053 - BT2306 0.19 -2.3 2,881,222 + BT2306 0.27 -2.4 2,881,223 - BT2306 0.27 +1.6 2,881,369 + BT2306 0.35 +1.3 2,881,369 + BT2306 0.35 +0.3 2,881,411 + BT2306 0.37 -0.5 2,881,412 - BT2306 0.37 -0.9 2,881,412 - BT2306 0.37 -2.9 2,881,412 - BT2306 0.37 -2.8 2,881,441 + BT2306 0.38 -3.8 2,881,444 - BT2306 0.38 -0.8 2,881,463 - BT2306 0.39 -1.0 2,881,469 + BT2306 0.40 -5.1 2,881,547 - BT2306 0.43 +0.0
Or see this region's nucleotide sequence