Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1949 and BT1950 overlap by 4 nucleotides BT1950 and BT1951 overlap by 37 nucleotides BT1951 and BT1952 are separated by 0 nucleotides
BT1949: BT1949 - conserved hypothetical protein (NCBI ptt file), at 2,448,514 to 2,449,080
BT1949
BT1950: BT1950 - Fe3+ ABC transporter, ATP-binding protein (NCBI ptt file), at 2,449,077 to 2,449,868
BT1950
BT1951: BT1951 - Fe3+ ABC transporter, permease protein (NCBI ptt file), at 2,449,832 to 2,450,812
BT1951
BT1952: BT1952 - Fe3+ ABC transporter, periplasmic iron-binding protein (NCBI ptt file), at 2,450,813 to 2,451,952
BT1952
Position (kb)
2449
2450 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2448.095 kb on - strand at 2448.100 kb on + strand at 2448.131 kb on - strand at 2448.131 kb on - strand at 2448.150 kb on + strand at 2448.181 kb on + strand at 2448.182 kb on - strand at 2448.182 kb on - strand at 2448.182 kb on - strand at 2448.182 kb on - strand at 2448.182 kb on - strand at 2448.189 kb on - strand at 2448.195 kb on - strand at 2448.195 kb on - strand at 2448.259 kb on - strand at 2448.279 kb on + strand at 2448.279 kb on + strand at 2448.280 kb on - strand at 2448.280 kb on - strand at 2448.292 kb on + strand at 2448.293 kb on - strand at 2448.359 kb on - strand at 2448.382 kb on + strand at 2448.383 kb on - strand at 2448.391 kb on - strand at 2448.403 kb on - strand at 2448.403 kb on - strand at 2448.428 kb on - strand at 2448.432 kb on - strand at 2448.436 kb on - strand at 2448.436 kb on - strand at 2448.436 kb on - strand at 2448.439 kb on + strand at 2448.440 kb on - strand at 2448.441 kb on + strand at 2448.483 kb on + strand at 2448.518 kb on + strand at 2448.518 kb on + strand at 2448.519 kb on - strand at 2448.524 kb on - strand at 2448.575 kb on + strand, within BT1949 at 2448.575 kb on + strand, within BT1949 at 2448.576 kb on - strand, within BT1949 at 2448.576 kb on - strand, within BT1949 at 2448.576 kb on - strand, within BT1949 at 2448.618 kb on + strand, within BT1949 at 2448.747 kb on + strand, within BT1949 at 2448.751 kb on + strand, within BT1949 at 2448.751 kb on + strand, within BT1949 at 2448.753 kb on + strand, within BT1949 at 2448.756 kb on - strand, within BT1949 at 2448.776 kb on + strand, within BT1949 at 2448.776 kb on + strand, within BT1949 at 2448.777 kb on - strand, within BT1949 at 2448.826 kb on + strand, within BT1949 at 2448.844 kb on - strand, within BT1949 at 2448.845 kb on + strand, within BT1949 at 2448.858 kb on + strand, within BT1949 at 2448.891 kb on + strand, within BT1949 at 2448.902 kb on + strand, within BT1949 at 2448.902 kb on + strand, within BT1949 at 2448.902 kb on + strand, within BT1949 at 2448.909 kb on - strand, within BT1949 at 2448.975 kb on + strand, within BT1949 at 2448.976 kb on - strand, within BT1949 at 2449.003 kb on + strand, within BT1949 at 2449.028 kb on + strand at 2449.070 kb on + strand at 2449.071 kb on - strand at 2449.072 kb on + strand at 2449.075 kb on - strand at 2449.150 kb on + strand at 2449.204 kb on - strand, within BT1950 at 2449.365 kb on - strand, within BT1950 at 2449.370 kb on + strand, within BT1950 at 2449.447 kb on + strand, within BT1950 at 2449.486 kb on - strand, within BT1950 at 2449.511 kb on + strand, within BT1950 at 2449.526 kb on + strand, within BT1950 at 2449.527 kb on - strand, within BT1950 at 2449.528 kb on + strand, within BT1950 at 2449.543 kb on - strand, within BT1950 at 2449.824 kb on + strand at 2449.897 kb on + strand at 2450.046 kb on - strand, within BT1951 at 2450.154 kb on + strand, within BT1951 at 2450.155 kb on - strand, within BT1951 at 2450.230 kb on + strand, within BT1951 at 2450.384 kb on + strand, within BT1951 at 2450.421 kb on - strand, within BT1951 at 2450.544 kb on + strand, within BT1951 at 2450.630 kb on - strand, within BT1951 at 2450.637 kb on - strand, within BT1951 at 2450.705 kb on + strand, within BT1951 at 2450.790 kb on - strand at 2450.793 kb on - strand at 2450.815 kb on - strand at 2450.829 kb on + strand at 2450.832 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 2,448,095 - +1.8 2,448,100 + -1.9 2,448,131 - -0.5 2,448,131 - +2.3 2,448,150 + -3.9 2,448,181 + -1.5 2,448,182 - -2.5 2,448,182 - -2.5 2,448,182 - -2.5 2,448,182 - -2.3 2,448,182 - -2.5 2,448,189 - -1.5 2,448,195 - +2.7 2,448,195 - -1.9 2,448,259 - -2.9 2,448,279 + -3.0 2,448,279 + +1.7 2,448,280 - +0.1 2,448,280 - -2.5 2,448,292 + +2.0 2,448,293 - -1.9 2,448,359 - -1.0 2,448,382 + -1.9 2,448,383 - +1.4 2,448,391 - -2.5 2,448,403 - -2.1 2,448,403 - -1.9 2,448,428 - +3.7 2,448,432 - +2.7 2,448,436 - -2.2 2,448,436 - -0.3 2,448,436 - -2.2 2,448,439 + -2.5 2,448,440 - -3.2 2,448,441 + -1.5 2,448,483 + +0.6 2,448,518 + +1.7 2,448,518 + +2.9 2,448,519 - +1.5 2,448,524 - -1.7 2,448,575 + BT1949 0.11 -3.9 2,448,575 + BT1949 0.11 -1.2 2,448,576 - BT1949 0.11 -2.3 2,448,576 - BT1949 0.11 +3.3 2,448,576 - BT1949 0.11 -2.6 2,448,618 + BT1949 0.18 +1.7 2,448,747 + BT1949 0.41 +1.9 2,448,751 + BT1949 0.42 -2.2 2,448,751 + BT1949 0.42 +2.2 2,448,753 + BT1949 0.42 -0.6 2,448,756 - BT1949 0.43 -1.4 2,448,776 + BT1949 0.46 -3.2 2,448,776 + BT1949 0.46 -2.7 2,448,777 - BT1949 0.46 -3.0 2,448,826 + BT1949 0.55 -1.6 2,448,844 - BT1949 0.58 -2.2 2,448,845 + BT1949 0.58 -4.0 2,448,858 + BT1949 0.61 -2.7 2,448,891 + BT1949 0.66 -1.6 2,448,902 + BT1949 0.68 -2.7 2,448,902 + BT1949 0.68 +0.5 2,448,902 + BT1949 0.68 -3.4 2,448,909 - BT1949 0.70 +2.1 2,448,975 + BT1949 0.81 +2.4 2,448,976 - BT1949 0.81 -3.2 2,449,003 + BT1949 0.86 -4.2 2,449,028 + +1.5 2,449,070 + -0.9 2,449,071 - -2.8 2,449,072 + +3.7 2,449,075 - -1.5 2,449,150 + -2.7 2,449,204 - BT1950 0.16 -2.9 2,449,365 - BT1950 0.36 -1.9 2,449,370 + BT1950 0.37 -3.4 2,449,447 + BT1950 0.47 -3.2 2,449,486 - BT1950 0.52 -2.2 2,449,511 + BT1950 0.55 +0.8 2,449,526 + BT1950 0.57 +0.1 2,449,527 - BT1950 0.57 -1.5 2,449,528 + BT1950 0.57 -4.0 2,449,543 - BT1950 0.59 +0.1 2,449,824 + -2.9 2,449,897 + -2.8 2,450,046 - BT1951 0.22 +0.3 2,450,154 + BT1951 0.33 -1.5 2,450,155 - BT1951 0.33 -0.9 2,450,230 + BT1951 0.41 -0.9 2,450,384 + BT1951 0.56 +0.1 2,450,421 - BT1951 0.60 +2.7 2,450,544 + BT1951 0.73 -0.6 2,450,630 - BT1951 0.81 -1.9 2,450,637 - BT1951 0.82 -1.5 2,450,705 + BT1951 0.89 -3.2 2,450,790 - -3.3 2,450,793 - -1.9 2,450,815 - -2.7 2,450,829 + -1.5 2,450,832 + -2.2
Or see this region's nucleotide sequence