Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1848 and BT1849 overlap by 8 nucleotides BT1849 and BT1850 overlap by 36 nucleotides
BT1848: BT1848 - ATP-dependent DNA helicase recQ (NCBI ptt file), at 2,320,195 to 2,322,057
BT1848
BT1849: BT1849 - hypothetical protein (NCBI ptt file), at 2,322,050 to 2,322,241
BT1849
BT1850: BT1850 - conserved hypothetical protein (NCBI ptt file), at 2,322,206 to 2,323,294
BT1850
Position (kb)
2322
2323 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2321.090 kb on + strand, within BT1848 at 2321.154 kb on + strand, within BT1848 at 2321.155 kb on - strand, within BT1848 at 2321.163 kb on + strand, within BT1848 at 2321.217 kb on - strand, within BT1848 at 2321.227 kb on - strand, within BT1848 at 2321.231 kb on + strand, within BT1848 at 2321.232 kb on - strand, within BT1848 at 2321.502 kb on - strand, within BT1848 at 2321.506 kb on - strand, within BT1848 at 2321.506 kb on - strand, within BT1848 at 2321.514 kb on - strand, within BT1848 at 2321.516 kb on + strand, within BT1848 at 2321.517 kb on - strand, within BT1848 at 2321.576 kb on + strand, within BT1848 at 2321.576 kb on + strand, within BT1848 at 2321.603 kb on + strand, within BT1848 at 2321.648 kb on + strand, within BT1848 at 2321.664 kb on - strand, within BT1848 at 2321.668 kb on + strand, within BT1848 at 2321.911 kb on - strand at 2321.962 kb on - strand at 2322.055 kb on + strand at 2322.064 kb on - strand at 2322.100 kb on + strand, within BT1849 at 2322.131 kb on + strand, within BT1849 at 2322.136 kb on + strand, within BT1849 at 2322.208 kb on - strand at 2322.208 kb on - strand at 2322.244 kb on + strand at 2322.245 kb on - strand at 2322.260 kb on - strand at 2322.278 kb on - strand at 2322.278 kb on - strand at 2322.289 kb on + strand at 2322.363 kb on - strand, within BT1850 at 2322.364 kb on + strand, within BT1850 at 2322.365 kb on - strand, within BT1850 at 2322.370 kb on + strand, within BT1850 at 2322.371 kb on - strand, within BT1850 at 2322.375 kb on + strand, within BT1850 at 2322.375 kb on + strand, within BT1850 at 2322.375 kb on + strand, within BT1850 at 2322.379 kb on + strand, within BT1850 at 2322.400 kb on + strand, within BT1850 at 2322.401 kb on - strand, within BT1850 at 2322.435 kb on - strand, within BT1850 at 2322.437 kb on - strand, within BT1850 at 2322.458 kb on - strand, within BT1850 at 2322.458 kb on - strand, within BT1850 at 2322.482 kb on - strand, within BT1850 at 2322.504 kb on - strand, within BT1850 at 2322.504 kb on - strand, within BT1850 at 2322.521 kb on - strand, within BT1850 at 2322.548 kb on + strand, within BT1850 at 2322.548 kb on + strand, within BT1850 at 2322.566 kb on - strand, within BT1850 at 2322.602 kb on + strand, within BT1850 at 2322.603 kb on - strand, within BT1850 at 2322.638 kb on + strand, within BT1850 at 2322.644 kb on + strand, within BT1850 at 2322.645 kb on - strand, within BT1850 at 2322.645 kb on - strand, within BT1850 at 2322.645 kb on - strand, within BT1850 at 2322.648 kb on + strand, within BT1850 at 2322.648 kb on + strand, within BT1850 at 2322.649 kb on - strand, within BT1850 at 2322.687 kb on + strand, within BT1850 at 2322.697 kb on + strand, within BT1850 at 2322.697 kb on + strand, within BT1850 at 2322.698 kb on - strand, within BT1850 at 2322.698 kb on - strand, within BT1850 at 2322.702 kb on + strand, within BT1850 at 2322.754 kb on - strand, within BT1850 at 2322.771 kb on - strand, within BT1850 at 2322.833 kb on + strand, within BT1850 at 2322.835 kb on + strand, within BT1850 at 2322.835 kb on + strand, within BT1850 at 2322.836 kb on - strand, within BT1850 at 2322.836 kb on - strand, within BT1850 at 2322.838 kb on + strand, within BT1850 at 2322.838 kb on + strand, within BT1850 at 2322.839 kb on - strand, within BT1850 at 2322.842 kb on + strand, within BT1850 at 2322.926 kb on - strand, within BT1850 at 2322.980 kb on - strand, within BT1850 at 2322.980 kb on - strand, within BT1850 at 2322.980 kb on - strand, within BT1850 at 2322.980 kb on - strand, within BT1850 at 2322.981 kb on + strand, within BT1850 at 2322.981 kb on + strand, within BT1850 at 2322.982 kb on - strand, within BT1850 at 2322.982 kb on - strand, within BT1850 at 2322.986 kb on + strand, within BT1850 at 2322.987 kb on - strand, within BT1850 at 2322.992 kb on - strand, within BT1850 at 2322.998 kb on - strand, within BT1850 at 2323.016 kb on - strand, within BT1850 at 2323.096 kb on + strand, within BT1850 at 2323.096 kb on + strand, within BT1850 at 2323.131 kb on + strand, within BT1850 at 2323.132 kb on - strand, within BT1850 at 2323.167 kb on - strand, within BT1850 at 2323.212 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 2,321,090 + BT1848 0.48 -3.5 2,321,154 + BT1848 0.51 -1.5 2,321,155 - BT1848 0.52 -0.9 2,321,163 + BT1848 0.52 +0.9 2,321,217 - BT1848 0.55 -3.1 2,321,227 - BT1848 0.55 -1.8 2,321,231 + BT1848 0.56 -1.5 2,321,232 - BT1848 0.56 -3.0 2,321,502 - BT1848 0.70 -3.3 2,321,506 - BT1848 0.70 -2.9 2,321,506 - BT1848 0.70 +1.1 2,321,514 - BT1848 0.71 +1.5 2,321,516 + BT1848 0.71 -1.5 2,321,517 - BT1848 0.71 +1.4 2,321,576 + BT1848 0.74 -2.9 2,321,576 + BT1848 0.74 -0.9 2,321,603 + BT1848 0.76 -2.2 2,321,648 + BT1848 0.78 -2.2 2,321,664 - BT1848 0.79 -1.3 2,321,668 + BT1848 0.79 -2.7 2,321,911 - +0.1 2,321,962 - -2.1 2,322,055 + -2.6 2,322,064 - +1.3 2,322,100 + BT1849 0.26 +3.1 2,322,131 + BT1849 0.42 -1.3 2,322,136 + BT1849 0.45 +2.1 2,322,208 - +2.1 2,322,208 - -2.8 2,322,244 + -1.8 2,322,245 - -1.9 2,322,260 - +0.8 2,322,278 - -3.8 2,322,278 - -2.8 2,322,289 + +2.9 2,322,363 - BT1850 0.14 -2.8 2,322,364 + BT1850 0.15 -1.3 2,322,365 - BT1850 0.15 -2.7 2,322,370 + BT1850 0.15 -2.6 2,322,371 - BT1850 0.15 +1.4 2,322,375 + BT1850 0.16 -1.4 2,322,375 + BT1850 0.16 -4.4 2,322,375 + BT1850 0.16 -1.6 2,322,379 + BT1850 0.16 +2.3 2,322,400 + BT1850 0.18 +2.3 2,322,401 - BT1850 0.18 -2.8 2,322,435 - BT1850 0.21 +1.1 2,322,437 - BT1850 0.21 +2.9 2,322,458 - BT1850 0.23 -2.9 2,322,458 - BT1850 0.23 -2.6 2,322,482 - BT1850 0.25 +1.7 2,322,504 - BT1850 0.27 -2.2 2,322,504 - BT1850 0.27 +0.1 2,322,521 - BT1850 0.29 -1.6 2,322,548 + BT1850 0.31 -4.2 2,322,548 + BT1850 0.31 -3.8 2,322,566 - BT1850 0.33 -0.5 2,322,602 + BT1850 0.36 -2.5 2,322,603 - BT1850 0.36 +1.7 2,322,638 + BT1850 0.40 -2.8 2,322,644 + BT1850 0.40 +3.4 2,322,645 - BT1850 0.40 -0.9 2,322,645 - BT1850 0.40 -2.6 2,322,645 - BT1850 0.40 +0.8 2,322,648 + BT1850 0.41 +0.9 2,322,648 + BT1850 0.41 +0.8 2,322,649 - BT1850 0.41 +1.4 2,322,687 + BT1850 0.44 -3.4 2,322,697 + BT1850 0.45 -2.2 2,322,697 + BT1850 0.45 -2.7 2,322,698 - BT1850 0.45 -1.7 2,322,698 - BT1850 0.45 -2.1 2,322,702 + BT1850 0.46 -2.2 2,322,754 - BT1850 0.50 +0.1 2,322,771 - BT1850 0.52 +2.3 2,322,833 + BT1850 0.58 -2.9 2,322,835 + BT1850 0.58 -0.7 2,322,835 + BT1850 0.58 +1.4 2,322,836 - BT1850 0.58 +0.9 2,322,836 - BT1850 0.58 +0.8 2,322,838 + BT1850 0.58 +2.6 2,322,838 + BT1850 0.58 -2.2 2,322,839 - BT1850 0.58 -2.9 2,322,842 + BT1850 0.58 -2.0 2,322,926 - BT1850 0.66 +0.5 2,322,980 - BT1850 0.71 -1.8 2,322,980 - BT1850 0.71 -2.2 2,322,980 - BT1850 0.71 -1.5 2,322,980 - BT1850 0.71 +2.3 2,322,981 + BT1850 0.71 -1.5 2,322,981 + BT1850 0.71 +1.7 2,322,982 - BT1850 0.71 -2.0 2,322,982 - BT1850 0.71 -1.5 2,322,986 + BT1850 0.72 +1.7 2,322,987 - BT1850 0.72 -2.7 2,322,992 - BT1850 0.72 -1.0 2,322,998 - BT1850 0.73 -2.7 2,323,016 - BT1850 0.74 -2.2 2,323,096 + BT1850 0.82 -2.1 2,323,096 + BT1850 0.82 -1.4 2,323,131 + BT1850 0.85 -1.7 2,323,132 - BT1850 0.85 -1.9 2,323,167 - BT1850 0.88 +2.2 2,323,212 + -2.1
Or see this region's nucleotide sequence