Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1527 and BT1528 are separated by 107 nucleotides BT1528 and BT1529 are separated by 21 nucleotides
BT1527: BT1527 - hypothetical protein (NCBI ptt file), at 1,881,405 to 1,883,957
BT1527
BT1528: BT1528 - two-component system sensor histidine kinase (NCBI ptt file), at 1,884,065 to 1,885,357
BT1528
BT1529: BT1529 - two-component system response regulator (NCBI ptt file), at 1,885,379 to 1,886,728
BT1529
Position (kb)
1884
1885
1886 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1883.187 kb on - strand, within BT1527 at 1883.243 kb on + strand, within BT1527 at 1883.258 kb on + strand, within BT1527 at 1883.342 kb on - strand, within BT1527 at 1883.412 kb on + strand, within BT1527 at 1883.413 kb on - strand, within BT1527 at 1883.413 kb on - strand, within BT1527 at 1883.446 kb on - strand, within BT1527 at 1883.632 kb on - strand, within BT1527 at 1883.647 kb on + strand, within BT1527 at 1883.672 kb on + strand, within BT1527 at 1883.674 kb on + strand, within BT1527 at 1883.718 kb on - strand at 1883.720 kb on - strand at 1883.823 kb on + strand at 1883.829 kb on + strand at 1883.830 kb on - strand at 1883.834 kb on - strand at 1883.834 kb on - strand at 1883.881 kb on - strand at 1883.881 kb on - strand at 1883.884 kb on + strand at 1883.917 kb on - strand at 1883.942 kb on + strand at 1883.943 kb on - strand at 1883.962 kb on + strand at 1883.963 kb on - strand at 1883.963 kb on - strand at 1883.964 kb on + strand at 1884.030 kb on - strand at 1884.030 kb on - strand at 1884.031 kb on + strand at 1884.105 kb on + strand at 1884.345 kb on - strand, within BT1528 at 1884.414 kb on - strand, within BT1528 at 1884.458 kb on - strand, within BT1528 at 1884.586 kb on + strand, within BT1528 at 1884.624 kb on - strand, within BT1528 at 1884.794 kb on - strand, within BT1528 at 1884.800 kb on - strand, within BT1528 at 1884.806 kb on - strand, within BT1528 at 1884.881 kb on + strand, within BT1528 at 1885.062 kb on - strand, within BT1528 at 1885.111 kb on - strand, within BT1528 at 1885.177 kb on + strand, within BT1528 at 1885.222 kb on - strand, within BT1528 at 1885.257 kb on + strand at 1885.331 kb on + strand at 1885.350 kb on - strand at 1885.357 kb on + strand at 1885.358 kb on - strand at 1885.366 kb on + strand at 1885.366 kb on + strand at 1885.366 kb on + strand at 1885.414 kb on - strand at 1885.444 kb on + strand at 1885.449 kb on - strand at 1885.523 kb on - strand, within BT1529 at 1885.628 kb on - strand, within BT1529 at 1885.628 kb on - strand, within BT1529 at 1885.673 kb on + strand, within BT1529 at 1885.825 kb on + strand, within BT1529 at 1885.938 kb on - strand, within BT1529 at 1886.074 kb on + strand, within BT1529 at 1886.074 kb on + strand, within BT1529 at 1886.183 kb on + strand, within BT1529 at 1886.256 kb on - strand, within BT1529 at 1886.277 kb on - strand, within BT1529
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 1,883,187 - BT1527 0.70 -2.7 1,883,243 + BT1527 0.72 -1.1 1,883,258 + BT1527 0.73 -1.5 1,883,342 - BT1527 0.76 -2.5 1,883,412 + BT1527 0.79 +1.9 1,883,413 - BT1527 0.79 -2.7 1,883,413 - BT1527 0.79 -1.1 1,883,446 - BT1527 0.80 -3.2 1,883,632 - BT1527 0.87 -2.0 1,883,647 + BT1527 0.88 +2.2 1,883,672 + BT1527 0.89 -0.5 1,883,674 + BT1527 0.89 +0.9 1,883,718 - -3.1 1,883,720 - +1.5 1,883,823 + +3.1 1,883,829 + -0.4 1,883,830 - -2.1 1,883,834 - -1.9 1,883,834 - -3.8 1,883,881 - +0.6 1,883,881 - -2.7 1,883,884 + -2.5 1,883,917 - -2.5 1,883,942 + -1.1 1,883,943 - -1.3 1,883,962 + -1.3 1,883,963 - +0.9 1,883,963 - -2.9 1,883,964 + -0.1 1,884,030 - -1.8 1,884,030 - -2.4 1,884,031 + -2.4 1,884,105 + -3.2 1,884,345 - BT1528 0.22 -1.5 1,884,414 - BT1528 0.27 -2.8 1,884,458 - BT1528 0.30 -1.7 1,884,586 + BT1528 0.40 +0.2 1,884,624 - BT1528 0.43 -3.4 1,884,794 - BT1528 0.56 -2.9 1,884,800 - BT1528 0.57 +2.4 1,884,806 - BT1528 0.57 -3.3 1,884,881 + BT1528 0.63 -2.3 1,885,062 - BT1528 0.77 -1.1 1,885,111 - BT1528 0.81 -1.8 1,885,177 + BT1528 0.86 +2.2 1,885,222 - BT1528 0.89 -4.9 1,885,257 + +0.5 1,885,331 + -2.4 1,885,350 - -1.8 1,885,357 + -3.1 1,885,358 - +1.2 1,885,366 + +0.3 1,885,366 + -0.7 1,885,366 + -0.6 1,885,414 - -3.2 1,885,444 + +3.6 1,885,449 - -1.8 1,885,523 - BT1529 0.11 -0.1 1,885,628 - BT1529 0.18 -2.5 1,885,628 - BT1529 0.18 -2.7 1,885,673 + BT1529 0.22 -2.4 1,885,825 + BT1529 0.33 -3.1 1,885,938 - BT1529 0.41 -3.7 1,886,074 + BT1529 0.51 +1.9 1,886,074 + BT1529 0.51 -3.2 1,886,183 + BT1529 0.60 +1.9 1,886,256 - BT1529 0.65 -3.4 1,886,277 - BT1529 0.67 -2.1
Or see this region's nucleotide sequence