Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1233 and BT1234 are separated by 100 nucleotides BT1234 and BT1235 are separated by 17 nucleotides
BT1233: BT1233 - hypothetical protein (NCBI ptt file), at 1,534,811 to 1,535,545
BT1233
BT1234: BT1234 - conserved hypothetical protein (NCBI ptt file), at 1,535,646 to 1,536,230
BT1234
BT1235: BT1235 - AraC-type transcription regulator (NCBI ptt file), at 1,536,248 to 1,537,150
BT1235
Position (kb)
1535
1536
1537 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1534.745 kb on + strand at 1534.809 kb on - strand at 1534.812 kb on + strand at 1534.921 kb on - strand, within BT1233 at 1534.923 kb on - strand, within BT1233 at 1535.177 kb on - strand, within BT1233 at 1535.182 kb on + strand, within BT1233 at 1535.206 kb on - strand, within BT1233 at 1535.219 kb on - strand, within BT1233 at 1535.248 kb on + strand, within BT1233 at 1535.352 kb on - strand, within BT1233 at 1535.399 kb on + strand, within BT1233 at 1535.399 kb on + strand, within BT1233 at 1535.400 kb on - strand, within BT1233 at 1535.466 kb on + strand, within BT1233 at 1535.467 kb on - strand, within BT1233 at 1535.468 kb on + strand, within BT1233 at 1535.545 kb on + strand at 1535.546 kb on - strand at 1535.547 kb on + strand at 1535.573 kb on - strand at 1535.573 kb on - strand at 1535.614 kb on - strand at 1535.651 kb on + strand at 1535.708 kb on + strand, within BT1234 at 1535.728 kb on + strand, within BT1234 at 1535.836 kb on - strand, within BT1234 at 1535.968 kb on - strand, within BT1234 at 1536.228 kb on + strand at 1536.228 kb on + strand at 1536.237 kb on - strand at 1536.250 kb on - strand at 1536.280 kb on + strand at 1536.295 kb on + strand at 1536.295 kb on + strand at 1536.296 kb on - strand at 1536.300 kb on + strand at 1536.305 kb on - strand at 1536.345 kb on - strand, within BT1235 at 1536.520 kb on - strand, within BT1235 at 1536.556 kb on + strand, within BT1235 at 1536.557 kb on - strand, within BT1235 at 1536.607 kb on - strand, within BT1235 at 1536.679 kb on + strand, within BT1235 at 1536.679 kb on + strand, within BT1235 at 1536.680 kb on - strand, within BT1235 at 1536.721 kb on - strand, within BT1235 at 1536.721 kb on - strand, within BT1235 at 1536.802 kb on + strand, within BT1235 at 1536.953 kb on - strand, within BT1235 at 1536.956 kb on - strand, within BT1235 at 1537.056 kb on - strand, within BT1235 at 1537.078 kb on + strand at 1537.094 kb on - strand at 1537.100 kb on - strand at 1537.120 kb on + strand at 1537.121 kb on - strand at 1537.129 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 1,534,745 + +1.1 1,534,809 - -1.1 1,534,812 + +0.3 1,534,921 - BT1233 0.15 -1.0 1,534,923 - BT1233 0.15 -1.3 1,535,177 - BT1233 0.50 -3.4 1,535,182 + BT1233 0.50 -0.6 1,535,206 - BT1233 0.54 -1.0 1,535,219 - BT1233 0.56 -1.5 1,535,248 + BT1233 0.59 +2.8 1,535,352 - BT1233 0.74 -0.3 1,535,399 + BT1233 0.80 -1.4 1,535,399 + BT1233 0.80 -4.6 1,535,400 - BT1233 0.80 +2.9 1,535,466 + BT1233 0.89 -2.3 1,535,467 - BT1233 0.89 -1.9 1,535,468 + BT1233 0.89 -1.2 1,535,545 + +1.6 1,535,546 - +2.9 1,535,547 + -2.1 1,535,573 - +0.9 1,535,573 - -1.6 1,535,614 - -2.6 1,535,651 + -2.0 1,535,708 + BT1234 0.11 -2.9 1,535,728 + BT1234 0.14 -3.4 1,535,836 - BT1234 0.32 -3.1 1,535,968 - BT1234 0.55 -3.2 1,536,228 + -1.7 1,536,228 + -3.6 1,536,237 - +0.7 1,536,250 - -1.3 1,536,280 + -0.3 1,536,295 + -1.3 1,536,295 + -1.3 1,536,296 - -2.1 1,536,300 + -1.3 1,536,305 - -4.3 1,536,345 - BT1235 0.11 -2.8 1,536,520 - BT1235 0.30 -0.6 1,536,556 + BT1235 0.34 -0.3 1,536,557 - BT1235 0.34 -2.4 1,536,607 - BT1235 0.40 +1.0 1,536,679 + BT1235 0.48 -2.3 1,536,679 + BT1235 0.48 -2.9 1,536,680 - BT1235 0.48 -0.0 1,536,721 - BT1235 0.52 -2.9 1,536,721 - BT1235 0.52 -0.3 1,536,802 + BT1235 0.61 -1.9 1,536,953 - BT1235 0.78 -0.1 1,536,956 - BT1235 0.78 -3.4 1,537,056 - BT1235 0.89 -1.3 1,537,078 + -1.9 1,537,094 - +0.7 1,537,100 - -3.0 1,537,120 + -2.3 1,537,121 - -4.2 1,537,129 + +0.0
Or see this region's nucleotide sequence