Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1192 and BT1193 are separated by 91 nucleotides BT1193 and BT1194 are separated by 61 nucleotides BT1194 and BT1195 overlap by 23 nucleotides
BT1192: BT1192 - putative xylanase (NCBI ptt file), at 1,487,562 to 1,488,953
BT1192
BT1193: BT1193 - conserved hypothetical protein (NCBI ptt file), at 1,489,045 to 1,490,400
BT1193
BT1194: BT1194 - outer membrane protein, OmpA family (NCBI ptt file), at 1,490,462 to 1,491,262
BT1194
BT1195: BT1195 - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI ptt file), at 1,491,240 to 1,491,986
BT1195
Position (kb)
1489
1490
1491 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1488.071 kb on - strand, within BT1192 at 1488.111 kb on - strand, within BT1192 at 1488.285 kb on - strand, within BT1192 at 1488.302 kb on - strand, within BT1192 at 1488.588 kb on - strand, within BT1192 at 1488.628 kb on - strand, within BT1192 at 1488.925 kb on - strand at 1488.954 kb on - strand at 1488.963 kb on - strand at 1488.965 kb on - strand at 1489.044 kb on + strand at 1489.129 kb on - strand at 1489.130 kb on + strand at 1489.251 kb on + strand, within BT1193 at 1489.274 kb on + strand, within BT1193 at 1489.275 kb on - strand, within BT1193 at 1489.408 kb on + strand, within BT1193 at 1489.416 kb on - strand, within BT1193 at 1489.507 kb on - strand, within BT1193 at 1489.544 kb on - strand, within BT1193 at 1489.572 kb on + strand, within BT1193 at 1489.813 kb on - strand, within BT1193 at 1490.099 kb on - strand, within BT1193 at 1490.115 kb on - strand, within BT1193 at 1490.236 kb on - strand, within BT1193 at 1490.425 kb on + strand at 1490.425 kb on + strand at 1490.426 kb on - strand at 1490.444 kb on + strand at 1490.477 kb on - strand at 1490.503 kb on - strand at 1490.582 kb on - strand, within BT1194 at 1490.671 kb on + strand, within BT1194 at 1490.672 kb on - strand, within BT1194 at 1490.817 kb on + strand, within BT1194 at 1490.984 kb on + strand, within BT1194 at 1490.985 kb on - strand, within BT1194 at 1490.993 kb on - strand, within BT1194 at 1490.993 kb on - strand, within BT1194 at 1491.209 kb on + strand at 1491.210 kb on - strand at 1491.215 kb on + strand at 1491.215 kb on + strand at 1491.216 kb on - strand at 1491.352 kb on - strand, within BT1195 at 1491.352 kb on - strand, within BT1195 at 1491.352 kb on - strand, within BT1195 at 1491.383 kb on - strand, within BT1195
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 1,488,071 - BT1192 0.37 -3.5 1,488,111 - BT1192 0.39 -2.4 1,488,285 - BT1192 0.52 -3.5 1,488,302 - BT1192 0.53 -3.8 1,488,588 - BT1192 0.74 -1.8 1,488,628 - BT1192 0.77 -5.3 1,488,925 - -1.8 1,488,954 - -3.1 1,488,963 - -2.1 1,488,965 - -1.3 1,489,044 + -3.0 1,489,129 - -5.2 1,489,130 + -4.2 1,489,251 + BT1193 0.15 -2.9 1,489,274 + BT1193 0.17 -4.1 1,489,275 - BT1193 0.17 -2.6 1,489,408 + BT1193 0.27 -4.8 1,489,416 - BT1193 0.27 -5.5 1,489,507 - BT1193 0.34 -4.0 1,489,544 - BT1193 0.37 -4.4 1,489,572 + BT1193 0.39 -0.7 1,489,813 - BT1193 0.57 -4.4 1,490,099 - BT1193 0.78 -2.6 1,490,115 - BT1193 0.79 -3.5 1,490,236 - BT1193 0.88 -2.6 1,490,425 + -0.7 1,490,425 + -0.3 1,490,426 - -3.1 1,490,444 + -2.1 1,490,477 - -4.8 1,490,503 - -4.1 1,490,582 - BT1194 0.15 -1.3 1,490,671 + BT1194 0.26 -3.5 1,490,672 - BT1194 0.26 -2.0 1,490,817 + BT1194 0.44 -1.3 1,490,984 + BT1194 0.65 -0.9 1,490,985 - BT1194 0.65 -2.9 1,490,993 - BT1194 0.66 +1.1 1,490,993 - BT1194 0.66 -1.9 1,491,209 + -2.8 1,491,210 - -0.9 1,491,215 + -1.9 1,491,215 + +1.4 1,491,216 - +0.7 1,491,352 - BT1195 0.15 -0.5 1,491,352 - BT1195 0.15 -0.7 1,491,352 - BT1195 0.15 -0.3 1,491,383 - BT1195 0.19 -1.9
Or see this region's nucleotide sequence