Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0704 and BT0705 are separated by 89 nucleotides BT0705 and BT0706 are separated by 286 nucleotides BT0706 and BT0707 are separated by 145 nucleotides
BT0704: BT0704 - phosphoribosylglycinamide formyltransferase 2 (NCBI ptt file), at 877,494 to 878,660
BT0704
BT0705: BT0705 - hypothetical protein (NCBI ptt file), at 878,750 to 879,697
BT0705
BT0706: BT0706 - putative N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 879,984 to 880,367
BT0706
BT0707: BT0707 - conserved hypothetical protein (NCBI ptt file), at 880,513 to 881,001
BT0707
Position (kb) 878
879
880 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 877.998 kb on + strand, within BT0704 at 878.520 kb on + strand, within BT0704 at 878.575 kb on + strand at 878.576 kb on - strand at 878.655 kb on + strand at 878.658 kb on + strand at 878.697 kb on + strand at 878.740 kb on - strand at 878.748 kb on + strand at 878.785 kb on + strand at 878.786 kb on - strand at 878.786 kb on - strand at 878.796 kb on + strand at 878.818 kb on + strand at 878.897 kb on - strand, within BT0705 at 878.951 kb on - strand, within BT0705 at 879.141 kb on + strand, within BT0705 at 879.258 kb on - strand, within BT0705 at 879.321 kb on - strand, within BT0705 at 879.338 kb on - strand, within BT0705 at 879.409 kb on + strand, within BT0705 at 879.411 kb on + strand, within BT0705 at 879.411 kb on + strand, within BT0705 at 879.412 kb on - strand, within BT0705 at 879.457 kb on - strand, within BT0705 at 879.548 kb on + strand, within BT0705 at 879.548 kb on + strand, within BT0705 at 879.549 kb on - strand, within BT0705 at 879.689 kb on + strand at 879.762 kb on - strand at 879.805 kb on - strand at 879.853 kb on - strand at 879.853 kb on - strand at 880.324 kb on + strand, within BT0706 at 880.370 kb on + strand at 880.448 kb on + strand at 880.463 kb on + strand at 880.514 kb on + strand at 880.666 kb on + strand, within BT0707
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 877,998 + BT0704 0.43 -2.0 878,520 + BT0704 0.88 +0.6 878,575 + -0.8 878,576 - -3.0 878,655 + -0.4 878,658 + -1.4 878,697 + -1.7 878,740 - -0.2 878,748 + -1.0 878,785 + -2.8 878,786 - +1.8 878,786 - -2.2 878,796 + -3.6 878,818 + +0.9 878,897 - BT0705 0.16 -3.4 878,951 - BT0705 0.21 -2.0 879,141 + BT0705 0.41 -3.7 879,258 - BT0705 0.54 -2.7 879,321 - BT0705 0.60 -2.7 879,338 - BT0705 0.62 +0.6 879,409 + BT0705 0.70 -2.1 879,411 + BT0705 0.70 +2.5 879,411 + BT0705 0.70 -3.1 879,412 - BT0705 0.70 -1.7 879,457 - BT0705 0.75 -3.0 879,548 + BT0705 0.84 +1.2 879,548 + BT0705 0.84 -2.7 879,549 - BT0705 0.84 +1.2 879,689 + +2.0 879,762 - -3.3 879,805 - -4.0 879,853 - -2.1 879,853 - +3.0 880,324 + BT0706 0.89 -2.4 880,370 + -2.6 880,448 + -1.4 880,463 + -2.2 880,514 + -3.4 880,666 + BT0707 0.31 -0.4
Or see this region's nucleotide sequence