Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0246 and BT0247 are separated by 53 nucleotides BT0247 and BT0248 are separated by 6 nucleotides BT0248 and BT0249 are separated by 69 nucleotides BT0249 and BT0250 are separated by 25 nucleotides
BT0246: BT0246 - putative integral membrane protein, with calcineurin-like phosphoesterase domain (NCBI ptt file), at 272,167 to 273,333
BT0246
BT0247: BT0247 - hypothetical protein (NCBI ptt file), at 273,387 to 273,965
BT0247
BT0248: BT0248 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 273,972 to 274,481
BT0248
BT0249: BT0249 - 5-methyltetrahydrofolate--homocysteine methyltransferase or methionine synthase (NCBI ptt file), at 274,551 to 275,453
BT0249
BT0250: BT0250 - dihydroorotase (DHOase) (NCBI ptt file), at 275,479 to 276,816
BT0250
Position (kb) 273
274
275 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 272.978 kb on - strand, within BT0246 at 273.182 kb on + strand, within BT0246 at 273.279 kb on - strand at 273.333 kb on + strand at 273.405 kb on - strand at 273.974 kb on - strand at 274.008 kb on - strand at 274.097 kb on + strand, within BT0248 at 274.097 kb on + strand, within BT0248 at 274.097 kb on + strand, within BT0248 at 274.179 kb on + strand, within BT0248 at 274.348 kb on + strand, within BT0248 at 274.379 kb on + strand, within BT0248 at 274.380 kb on - strand, within BT0248 at 274.383 kb on + strand, within BT0248 at 274.387 kb on + strand, within BT0248 at 274.387 kb on + strand, within BT0248 at 274.392 kb on + strand, within BT0248 at 274.392 kb on + strand, within BT0248 at 274.392 kb on + strand, within BT0248 at 274.392 kb on + strand, within BT0248 at 274.393 kb on - strand, within BT0248 at 274.542 kb on - strand at 274.616 kb on - strand at 274.831 kb on + strand, within BT0249 at 274.899 kb on + strand, within BT0249 at 274.899 kb on + strand, within BT0249 at 274.903 kb on + strand, within BT0249 at 275.011 kb on - strand, within BT0249 at 275.011 kb on - strand, within BT0249 at 275.172 kb on - strand, within BT0249 at 275.402 kb on - strand at 275.434 kb on - strand at 275.438 kb on + strand at 275.438 kb on + strand at 275.439 kb on - strand at 275.439 kb on - strand at 275.439 kb on - strand at 275.453 kb on + strand at 275.454 kb on - strand at 275.459 kb on + strand at 275.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 272,978 - BT0246 0.69 +0.7 273,182 + BT0246 0.87 -3.3 273,279 - +0.7 273,333 + -3.5 273,405 - +1.9 273,974 - -0.3 274,008 - +1.9 274,097 + BT0248 0.25 -3.8 274,097 + BT0248 0.25 -2.3 274,097 + BT0248 0.25 -2.8 274,179 + BT0248 0.41 -4.5 274,348 + BT0248 0.74 -0.3 274,379 + BT0248 0.80 -1.3 274,380 - BT0248 0.80 -3.3 274,383 + BT0248 0.81 -1.9 274,387 + BT0248 0.81 +0.1 274,387 + BT0248 0.81 -1.3 274,392 + BT0248 0.82 -1.3 274,392 + BT0248 0.82 +0.7 274,392 + BT0248 0.82 -0.6 274,392 + BT0248 0.82 -0.0 274,393 - BT0248 0.83 -4.7 274,542 - +0.3 274,616 - -1.6 274,831 + BT0249 0.31 -3.7 274,899 + BT0249 0.39 -0.3 274,899 + BT0249 0.39 -2.6 274,903 + BT0249 0.39 -0.3 275,011 - BT0249 0.51 -0.9 275,011 - BT0249 0.51 -2.1 275,172 - BT0249 0.69 +2.7 275,402 - -2.5 275,434 - -2.8 275,438 + -3.6 275,438 + -4.4 275,439 - +2.9 275,439 - -0.9 275,439 - -0.8 275,453 + -2.9 275,454 - +1.3 275,459 + -0.3 275,460 - -3.0
Or see this region's nucleotide sequence