Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0164 and BT0165 are separated by 96 nucleotides BT0165 and BT0166 are separated by 15 nucleotides BT0166 and BT0167 are separated by 129 nucleotides BT0167 and BT0168 overlap by 8 nucleotides BT0168 and BT0169 are separated by 217 nucleotides
BT0164: BT0164 - conserved hypothetical protein (NCBI ptt file), at 169,983 to 175,358
BT0164
BT0165: BT0165 - peptidyl-prolyl cis-trans isomerase (NCBI ptt file), at 175,455 to 175,856
BT0165
BT0166: BT0166 - putative thiol-disulfide isomerase (NCBI ptt file), at 175,872 to 176,429
BT0166
BT0167: BT0167 - conserved hypothetical protein (NCBI ptt file), at 176,559 to 176,897
BT0167
BT0168: BT0168 - conserved hypothetical protein (NCBI ptt file), at 176,890 to 177,189
BT0168
BT0169: BT0169 - conserved hypothetical protein (NCBI ptt file), at 177,407 to 178,462
BT0169
Position (kb) 175
176
177 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 174.923 kb on + strand at 175.068 kb on - strand at 175.085 kb on - strand at 175.085 kb on - strand at 175.176 kb on + strand at 175.318 kb on - strand at 175.473 kb on + strand at 175.474 kb on - strand at 175.528 kb on - strand, within BT0165 at 175.549 kb on - strand, within BT0165 at 175.699 kb on + strand, within BT0165 at 175.700 kb on - strand, within BT0165 at 175.702 kb on + strand, within BT0165 at 175.703 kb on - strand, within BT0165 at 175.703 kb on - strand, within BT0165 at 175.703 kb on - strand, within BT0165 at 175.758 kb on + strand, within BT0165 at 175.758 kb on + strand, within BT0165 at 175.758 kb on + strand, within BT0165 at 175.762 kb on + strand, within BT0165 at 175.768 kb on - strand, within BT0165 at 175.826 kb on - strand at 175.854 kb on + strand at 175.854 kb on + strand at 175.855 kb on - strand at 175.858 kb on + strand at 175.885 kb on + strand at 175.885 kb on + strand at 175.904 kb on - strand at 175.909 kb on + strand at 175.909 kb on + strand at 175.909 kb on + strand at 175.909 kb on + strand at 175.913 kb on + strand at 175.913 kb on + strand at 175.914 kb on - strand at 175.941 kb on - strand, within BT0166 at 176.142 kb on + strand, within BT0166 at 176.144 kb on + strand, within BT0166 at 176.144 kb on + strand, within BT0166 at 176.144 kb on + strand, within BT0166 at 176.161 kb on + strand, within BT0166 at 176.162 kb on - strand, within BT0166 at 176.174 kb on + strand, within BT0166 at 176.300 kb on + strand, within BT0166 at 176.328 kb on - strand, within BT0166 at 176.339 kb on - strand, within BT0166 at 176.339 kb on - strand, within BT0166 at 176.342 kb on + strand, within BT0166 at 176.342 kb on + strand, within BT0166 at 176.343 kb on - strand, within BT0166 at 176.343 kb on - strand, within BT0166 at 176.343 kb on - strand, within BT0166 at 176.403 kb on + strand at 176.497 kb on - strand at 176.571 kb on + strand at 176.591 kb on + strand at 176.609 kb on + strand, within BT0167 at 176.635 kb on - strand, within BT0167 at 176.640 kb on + strand, within BT0167 at 176.640 kb on + strand, within BT0167 at 176.646 kb on - strand, within BT0167 at 176.651 kb on - strand, within BT0167 at 176.664 kb on - strand, within BT0167 at 176.665 kb on + strand, within BT0167 at 176.702 kb on + strand, within BT0167 at 176.703 kb on - strand, within BT0167 at 176.703 kb on - strand, within BT0167 at 176.732 kb on + strand, within BT0167 at 176.732 kb on + strand, within BT0167 at 176.733 kb on - strand, within BT0167 at 176.738 kb on + strand, within BT0167 at 176.757 kb on + strand, within BT0167 at 176.792 kb on + strand, within BT0167 at 176.793 kb on - strand, within BT0167 at 176.809 kb on + strand, within BT0167 at 176.883 kb on + strand at 177.154 kb on + strand, within BT0168 at 177.156 kb on + strand, within BT0168 at 177.217 kb on - strand at 177.217 kb on - strand at 177.289 kb on - strand at 177.365 kb on + strand at 177.365 kb on + strand at 177.366 kb on - strand at 177.366 kb on - strand at 177.369 kb on + strand at 177.370 kb on - strand at 177.370 kb on - strand at 177.401 kb on - strand at 177.427 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 174,923 + -2.7 175,068 - -2.5 175,085 - +2.4 175,085 - -1.6 175,176 + +2.1 175,318 - +2.0 175,473 + -1.9 175,474 - +1.2 175,528 - BT0165 0.18 +2.8 175,549 - BT0165 0.23 +0.2 175,699 + BT0165 0.61 -2.2 175,700 - BT0165 0.61 -0.2 175,702 + BT0165 0.61 -0.2 175,703 - BT0165 0.62 -2.2 175,703 - BT0165 0.62 -0.9 175,703 - BT0165 0.62 +0.1 175,758 + BT0165 0.75 -3.0 175,758 + BT0165 0.75 -1.9 175,758 + BT0165 0.75 -1.3 175,762 + BT0165 0.76 -2.8 175,768 - BT0165 0.78 -3.4 175,826 - +1.6 175,854 + -2.2 175,854 + -0.2 175,855 - +0.0 175,858 + +1.4 175,885 + -2.5 175,885 + -0.2 175,904 - -1.0 175,909 + -2.7 175,909 + -2.8 175,909 + -0.9 175,909 + +1.0 175,913 + +2.1 175,913 + +0.3 175,914 - +2.6 175,941 - BT0166 0.12 -1.0 176,142 + BT0166 0.48 -3.8 176,144 + BT0166 0.49 +1.8 176,144 + BT0166 0.49 +1.9 176,144 + BT0166 0.49 -2.1 176,161 + BT0166 0.52 +1.3 176,162 - BT0166 0.52 +3.0 176,174 + BT0166 0.54 +0.8 176,300 + BT0166 0.77 -0.9 176,328 - BT0166 0.82 -0.2 176,339 - BT0166 0.84 -1.6 176,339 - BT0166 0.84 -2.5 176,342 + BT0166 0.84 +2.0 176,342 + BT0166 0.84 +0.8 176,343 - BT0166 0.84 -1.2 176,343 - BT0166 0.84 +0.8 176,343 - BT0166 0.84 +0.8 176,403 + -1.5 176,497 - -4.7 176,571 + -3.6 176,591 + -2.8 176,609 + BT0167 0.15 -1.2 176,635 - BT0167 0.22 -1.8 176,640 + BT0167 0.24 -1.9 176,640 + BT0167 0.24 +1.8 176,646 - BT0167 0.26 -1.8 176,651 - BT0167 0.27 -3.9 176,664 - BT0167 0.31 +1.1 176,665 + BT0167 0.31 -1.5 176,702 + BT0167 0.42 -0.2 176,703 - BT0167 0.42 -1.7 176,703 - BT0167 0.42 -2.2 176,732 + BT0167 0.51 -3.7 176,732 + BT0167 0.51 +2.0 176,733 - BT0167 0.51 -2.7 176,738 + BT0167 0.53 -1.0 176,757 + BT0167 0.58 -1.8 176,792 + BT0167 0.69 -1.5 176,793 - BT0167 0.69 +0.2 176,809 + BT0167 0.74 +1.4 176,883 + +2.2 177,154 + BT0168 0.88 -1.7 177,156 + BT0168 0.89 +2.9 177,217 - -1.2 177,217 - +1.4 177,289 - +2.9 177,365 + +1.1 177,365 + -1.0 177,366 - +2.7 177,366 - -2.6 177,369 + -0.2 177,370 - -1.2 177,370 - -1.8 177,401 - -1.9 177,427 + -3.9
Or see this region's nucleotide sequence