Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08725

Experiment: L-Histidine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08720 and BWI76_RS08725 overlap by 4 nucleotidesBWI76_RS08725 and BWI76_RS08730 are separated by 53 nucleotidesBWI76_RS08730 and BWI76_RS08735 are separated by 143 nucleotides BWI76_RS08720: BWI76_RS08720 - imidazolonepropionase, at 1,836,606 to 1,837,823 _RS08720 BWI76_RS08725: BWI76_RS08725 - Formiminoglutamase (EC 3.5.3.8) (from data), at 1,837,820 to 1,838,776 _RS08725 BWI76_RS08730: BWI76_RS08730 - histidine utilization repressor, at 1,838,830 to 1,839,555 _RS08730 BWI76_RS08735: BWI76_RS08735 - Urocanate hydratase (EC 4.2.1.49) (from data), at 1,839,699 to 1,841,387 _RS08735 Position (kb) 1837 1838 1839Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1837.299 kb on + strand, within BWI76_RS08720at 1837.602 kb on + strand, within BWI76_RS08720at 1837.653 kb on - strand, within BWI76_RS08720at 1837.885 kb on - strandat 1838.181 kb on + strand, within BWI76_RS08725at 1838.229 kb on + strand, within BWI76_RS08725at 1838.484 kb on - strand, within BWI76_RS08725at 1838.668 kb on + strand, within BWI76_RS08725at 1838.668 kb on + strand, within BWI76_RS08725at 1839.414 kb on - strand, within BWI76_RS08730at 1839.414 kb on - strand, within BWI76_RS08730at 1839.482 kb on - strand, within BWI76_RS08730at 1839.640 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Histidine (N)
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1,837,299 + BWI76_RS08720 0.57 -3.5
1,837,602 + BWI76_RS08720 0.82 -2.0
1,837,653 - BWI76_RS08720 0.86 -3.4
1,837,885 - -4.1
1,838,181 + BWI76_RS08725 0.38 -2.6
1,838,229 + BWI76_RS08725 0.43 -3.7
1,838,484 - BWI76_RS08725 0.69 -3.2
1,838,668 + BWI76_RS08725 0.89 -4.1
1,838,668 + BWI76_RS08725 0.89 -4.4
1,839,414 - BWI76_RS08730 0.80 +0.2
1,839,414 - BWI76_RS08730 0.80 +0.7
1,839,482 - BWI76_RS08730 0.90 +0.4
1,839,640 - -2.5

Or see this region's nucleotide sequence