Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02505 and BWI76_RS02510 overlap by 2 nucleotides BWI76_RS02510 and BWI76_RS02515 overlap by 8 nucleotides BWI76_RS02515 and BWI76_RS02520 are separated by 16 nucleotides
BWI76_RS02505: BWI76_RS02505 - tRNA epoxyqueuosine(34) reductase QueG, at 512,543 to 513,682
_RS02505
BWI76_RS02510: BWI76_RS02510 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 513,681 to 515,207
_RS02510
BWI76_RS02515: BWI76_RS02515 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 515,200 to 515,661
_RS02515
BWI76_RS02520: BWI76_RS02520 - N-acetylmuramoyl-L-alanine amidase, at 515,678 to 517,015
_RS02520
Position (kb) 513
514
515
516 Strain fitness (log2 ratio) -1
0
1
2 at 512.689 kb on - strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.868 kb on + strand, within BWI76_RS02505 at 513.045 kb on + strand, within BWI76_RS02505 at 513.201 kb on - strand, within BWI76_RS02505 at 513.203 kb on + strand, within BWI76_RS02505 at 513.354 kb on - strand, within BWI76_RS02505 at 513.354 kb on - strand, within BWI76_RS02505 at 513.389 kb on - strand, within BWI76_RS02505 at 513.481 kb on + strand, within BWI76_RS02505 at 513.567 kb on - strand, within BWI76_RS02505 at 513.610 kb on + strand at 513.617 kb on + strand at 513.622 kb on + strand at 513.625 kb on - strand at 513.712 kb on + strand at 513.752 kb on - strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.786 kb on - strand at 513.913 kb on - strand, within BWI76_RS02510 at 513.935 kb on - strand, within BWI76_RS02510 at 514.099 kb on - strand at 514.101 kb on + strand, within BWI76_RS02510 at 514.101 kb on + strand, within BWI76_RS02510 at 514.104 kb on - strand, within BWI76_RS02510 at 514.109 kb on - strand, within BWI76_RS02510 at 514.156 kb on - strand, within BWI76_RS02510 at 514.194 kb on + strand, within BWI76_RS02510 at 514.203 kb on + strand, within BWI76_RS02510 at 514.250 kb on - strand, within BWI76_RS02510 at 514.310 kb on - strand, within BWI76_RS02510 at 514.327 kb on + strand, within BWI76_RS02510 at 514.639 kb on + strand, within BWI76_RS02510 at 514.666 kb on + strand, within BWI76_RS02510 at 514.698 kb on - strand, within BWI76_RS02510 at 515.007 kb on + strand, within BWI76_RS02510 at 515.054 kb on + strand, within BWI76_RS02510 at 515.689 kb on - strand at 515.816 kb on + strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 516.024 kb on + strand, within BWI76_RS02520 at 516.171 kb on - strand, within BWI76_RS02520 at 516.196 kb on + strand, within BWI76_RS02520 at 516.204 kb on - strand, within BWI76_RS02520
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 512,689 - BWI76_RS02505 0.13 -0.4 512,733 + BWI76_RS02505 0.17 +0.2 512,733 + BWI76_RS02505 0.17 +0.1 512,733 + BWI76_RS02505 0.17 +0.9 512,868 + BWI76_RS02505 0.29 -0.1 513,045 + BWI76_RS02505 0.44 -0.5 513,201 - BWI76_RS02505 0.58 -0.8 513,203 + BWI76_RS02505 0.58 -0.0 513,354 - BWI76_RS02505 0.71 -0.1 513,354 - BWI76_RS02505 0.71 -1.4 513,389 - BWI76_RS02505 0.74 -1.0 513,481 + BWI76_RS02505 0.82 -0.6 513,567 - BWI76_RS02505 0.90 -0.2 513,610 + -0.3 513,617 + -0.8 513,622 + +1.3 513,625 - +0.1 513,712 + +0.5 513,752 - +0.7 513,778 + +0.4 513,778 + +0.2 513,778 + -1.1 513,778 + +1.1 513,786 - -1.3 513,913 - BWI76_RS02510 0.15 +1.0 513,935 - BWI76_RS02510 0.17 +1.4 514,099 - +0.9 514,101 + BWI76_RS02510 0.28 +1.2 514,101 + BWI76_RS02510 0.28 +0.0 514,104 - BWI76_RS02510 0.28 -0.1 514,109 - BWI76_RS02510 0.28 -1.0 514,156 - BWI76_RS02510 0.31 -0.1 514,194 + BWI76_RS02510 0.34 -0.4 514,203 + BWI76_RS02510 0.34 +1.9 514,250 - BWI76_RS02510 0.37 +2.3 514,310 - BWI76_RS02510 0.41 +0.8 514,327 + BWI76_RS02510 0.42 +0.2 514,639 + BWI76_RS02510 0.63 +0.4 514,666 + BWI76_RS02510 0.65 +0.9 514,698 - BWI76_RS02510 0.67 +1.5 515,007 + BWI76_RS02510 0.87 +0.8 515,054 + BWI76_RS02510 0.90 -0.1 515,689 - +1.2 515,816 + BWI76_RS02520 0.10 +0.3 515,890 - BWI76_RS02520 0.16 -0.5 515,890 - BWI76_RS02520 0.16 +1.4 515,890 - BWI76_RS02520 0.16 +0.2 516,024 + BWI76_RS02520 0.26 +0.6 516,171 - BWI76_RS02520 0.37 +0.9 516,196 + BWI76_RS02520 0.39 -0.5 516,204 - BWI76_RS02520 0.39 -0.5
Or see this region's nucleotide sequence