Experiment: reconstituted MRS with ISOThrive-prebiotic lot C 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15555 and BBR_RS15560 are separated by 157 nucleotides BBR_RS15560 and BBR_RS15565 are separated by 500 nucleotides
BBR_RS15555: BBR_RS15555 - HAD family hydrolase, at 1,262,892 to 1,263,743
_RS15555
BBR_RS15560: BBR_RS15560 - aminotransferase class V-fold PLP-dependent enzyme, at 1,263,901 to 1,265,397
_RS15560
BBR_RS15565: BBR_RS15565 - hypothetical protein, at 1,265,898 to 1,267,082
_RS15565
Position (kb)
1263
1264
1265
1266 Strain fitness (log2 ratio)
-1
0
1 at 1262.902 kb on - strand at 1262.963 kb on - strand at 1263.007 kb on - strand, within BBR_RS15555 at 1263.135 kb on - strand, within BBR_RS15555 at 1263.140 kb on - strand, within BBR_RS15555 at 1263.187 kb on + strand, within BBR_RS15555 at 1263.195 kb on - strand, within BBR_RS15555 at 1263.241 kb on + strand, within BBR_RS15555 at 1263.515 kb on + strand, within BBR_RS15555 at 1263.653 kb on - strand, within BBR_RS15555 at 1263.663 kb on - strand at 1263.683 kb on + strand at 1263.891 kb on + strand at 1263.913 kb on + strand at 1263.941 kb on + strand at 1264.141 kb on + strand, within BBR_RS15560 at 1264.208 kb on - strand, within BBR_RS15560 at 1264.259 kb on + strand, within BBR_RS15560 at 1264.259 kb on + strand, within BBR_RS15560 at 1264.264 kb on + strand, within BBR_RS15560 at 1264.489 kb on + strand, within BBR_RS15560 at 1264.500 kb on + strand, within BBR_RS15560 at 1264.509 kb on + strand, within BBR_RS15560 at 1264.560 kb on + strand, within BBR_RS15560 at 1264.604 kb on + strand, within BBR_RS15560 at 1264.869 kb on + strand, within BBR_RS15560 at 1264.883 kb on + strand, within BBR_RS15560 at 1264.891 kb on - strand, within BBR_RS15560 at 1264.891 kb on - strand, within BBR_RS15560 at 1264.965 kb on + strand, within BBR_RS15560 at 1265.009 kb on + strand, within BBR_RS15560 at 1265.009 kb on + strand, within BBR_RS15560 at 1265.055 kb on + strand, within BBR_RS15560 at 1265.072 kb on + strand, within BBR_RS15560 at 1265.078 kb on - strand, within BBR_RS15560 at 1265.123 kb on - strand, within BBR_RS15560 at 1265.340 kb on + strand at 1265.340 kb on + strand at 1265.431 kb on + strand at 1265.473 kb on + strand at 1265.473 kb on + strand at 1265.531 kb on - strand at 1265.534 kb on + strand at 1265.546 kb on + strand at 1265.659 kb on - strand at 1265.807 kb on - strand at 1265.812 kb on + strand at 1265.815 kb on - strand at 1265.821 kb on + strand at 1265.896 kb on + strand at 1265.896 kb on + strand at 1265.927 kb on - strand at 1265.927 kb on - strand at 1265.939 kb on + strand at 1265.944 kb on + strand at 1265.944 kb on + strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.955 kb on - strand at 1265.971 kb on + strand at 1266.024 kb on + strand, within BBR_RS15565 at 1266.043 kb on - strand, within BBR_RS15565 at 1266.043 kb on - strand, within BBR_RS15565 at 1266.110 kb on + strand, within BBR_RS15565 at 1266.110 kb on + strand, within BBR_RS15565 at 1266.145 kb on - strand, within BBR_RS15565 at 1266.145 kb on - strand, within BBR_RS15565 at 1266.178 kb on + strand, within BBR_RS15565 at 1266.178 kb on + strand, within BBR_RS15565 at 1266.231 kb on - strand, within BBR_RS15565 at 1266.291 kb on - strand, within BBR_RS15565 at 1266.338 kb on - strand, within BBR_RS15565 at 1266.376 kb on + strand, within BBR_RS15565
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with ISOThrive-prebiotic lot C 0.5 %w/v remove 1,262,902 - -1.6 1,262,963 - +1.7 1,263,007 - BBR_RS15555 0.13 -0.1 1,263,135 - BBR_RS15555 0.29 +0.7 1,263,140 - BBR_RS15555 0.29 +0.9 1,263,187 + BBR_RS15555 0.35 +0.3 1,263,195 - BBR_RS15555 0.36 -0.4 1,263,241 + BBR_RS15555 0.41 -0.6 1,263,515 + BBR_RS15555 0.73 +1.3 1,263,653 - BBR_RS15555 0.89 -0.2 1,263,663 - +1.2 1,263,683 + +0.6 1,263,891 + +0.2 1,263,913 + +0.3 1,263,941 + -0.6 1,264,141 + BBR_RS15560 0.16 -1.4 1,264,208 - BBR_RS15560 0.21 -0.5 1,264,259 + BBR_RS15560 0.24 +0.3 1,264,259 + BBR_RS15560 0.24 -0.2 1,264,264 + BBR_RS15560 0.24 -0.4 1,264,489 + BBR_RS15560 0.39 -0.5 1,264,500 + BBR_RS15560 0.40 -0.1 1,264,509 + BBR_RS15560 0.41 -0.4 1,264,560 + BBR_RS15560 0.44 -0.4 1,264,604 + BBR_RS15560 0.47 -0.4 1,264,869 + BBR_RS15560 0.65 -0.6 1,264,883 + BBR_RS15560 0.66 +1.5 1,264,891 - BBR_RS15560 0.66 +0.6 1,264,891 - BBR_RS15560 0.66 +1.6 1,264,965 + BBR_RS15560 0.71 +0.3 1,265,009 + BBR_RS15560 0.74 -0.3 1,265,009 + BBR_RS15560 0.74 +0.1 1,265,055 + BBR_RS15560 0.77 -0.7 1,265,072 + BBR_RS15560 0.78 -0.8 1,265,078 - BBR_RS15560 0.79 +0.5 1,265,123 - BBR_RS15560 0.82 +0.8 1,265,340 + +1.0 1,265,340 + -0.5 1,265,431 + -0.5 1,265,473 + -0.6 1,265,473 + -0.3 1,265,531 - -0.7 1,265,534 + -0.4 1,265,546 + +0.3 1,265,659 - +0.3 1,265,807 - +0.3 1,265,812 + -0.4 1,265,815 - +0.6 1,265,821 + -1.7 1,265,896 + -1.7 1,265,896 + -0.2 1,265,927 - -0.4 1,265,927 - -0.5 1,265,939 + +0.1 1,265,944 + +0.2 1,265,944 + +0.1 1,265,955 - +0.2 1,265,955 - -0.5 1,265,955 - +0.2 1,265,955 - -0.8 1,265,971 + +1.3 1,266,024 + BBR_RS15565 0.11 +0.7 1,266,043 - BBR_RS15565 0.12 +0.0 1,266,043 - BBR_RS15565 0.12 +0.4 1,266,110 + BBR_RS15565 0.18 +0.2 1,266,110 + BBR_RS15565 0.18 +0.2 1,266,145 - BBR_RS15565 0.21 +0.7 1,266,145 - BBR_RS15565 0.21 -0.0 1,266,178 + BBR_RS15565 0.24 -1.0 1,266,178 + BBR_RS15565 0.24 -0.5 1,266,231 - BBR_RS15565 0.28 +0.1 1,266,291 - BBR_RS15565 0.33 +0.4 1,266,338 - BBR_RS15565 0.37 -0.4 1,266,376 + BBR_RS15565 0.40 +0.3
Or see this region's nucleotide sequence