Experiment: m.b. Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2526 and Dshi_2527 are separated by 441 nucleotides Dshi_2527 and Dshi_2528 are separated by 92 nucleotides Dshi_2528 and Dshi_2529 overlap by 23 nucleotides
Dshi_2526: Dshi_2526 - FAD-dependent pyridine nucleotide-disulphide oxidoreductase (RefSeq), at 2,686,988 to 2,688,289
_2526
Dshi_2527: Dshi_2527 - hypothetical protein (RefSeq), at 2,688,731 to 2,689,123
_2527
Dshi_2528: Dshi_2528 - putative transcriptional regulator, Crp/Fnr family (RefSeq), at 2,689,216 to 2,689,971
_2528
Dshi_2529: Dshi_2529 - hypothetical protein (RefSeq), at 2,689,949 to 2,691,163
_2529
Position (kb)
2688
2689
2690 Strain fitness (log2 ratio)
-1
0
1
2 at 2687.739 kb on + strand, within Dshi_2526 at 2687.780 kb on - strand, within Dshi_2526 at 2687.780 kb on - strand, within Dshi_2526 at 2687.832 kb on + strand, within Dshi_2526 at 2687.937 kb on + strand, within Dshi_2526 at 2687.945 kb on - strand, within Dshi_2526 at 2687.988 kb on - strand, within Dshi_2526 at 2688.000 kb on - strand, within Dshi_2526 at 2688.011 kb on + strand, within Dshi_2526 at 2688.064 kb on - strand, within Dshi_2526 at 2688.074 kb on - strand, within Dshi_2526 at 2688.079 kb on - strand, within Dshi_2526 at 2688.158 kb on - strand, within Dshi_2526 at 2688.201 kb on + strand at 2688.201 kb on + strand at 2688.209 kb on - strand at 2688.209 kb on - strand at 2688.209 kb on - strand at 2688.248 kb on + strand at 2688.318 kb on + strand at 2688.326 kb on + strand at 2688.326 kb on + strand at 2688.334 kb on - strand at 2688.334 kb on - strand at 2688.334 kb on - strand at 2688.384 kb on - strand at 2688.394 kb on + strand at 2688.395 kb on + strand at 2688.408 kb on - strand at 2688.440 kb on - strand at 2688.440 kb on - strand at 2688.450 kb on - strand at 2688.450 kb on - strand at 2688.599 kb on + strand at 2688.607 kb on - strand at 2688.702 kb on - strand at 2688.703 kb on + strand at 2688.764 kb on - strand at 2688.764 kb on - strand at 2688.800 kb on - strand, within Dshi_2527 at 2688.805 kb on - strand, within Dshi_2527 at 2688.805 kb on - strand, within Dshi_2527 at 2688.870 kb on - strand, within Dshi_2527 at 2688.920 kb on - strand, within Dshi_2527 at 2688.922 kb on + strand, within Dshi_2527 at 2688.956 kb on - strand, within Dshi_2527 at 2689.009 kb on + strand, within Dshi_2527 at 2689.015 kb on + strand, within Dshi_2527 at 2689.070 kb on + strand, within Dshi_2527 at 2689.086 kb on - strand at 2689.183 kb on + strand at 2689.204 kb on + strand at 2689.222 kb on - strand at 2689.260 kb on + strand at 2689.273 kb on - strand at 2689.280 kb on + strand at 2689.324 kb on + strand, within Dshi_2528 at 2689.356 kb on + strand, within Dshi_2528 at 2689.396 kb on + strand, within Dshi_2528 at 2689.453 kb on - strand, within Dshi_2528 at 2689.626 kb on - strand, within Dshi_2528 at 2689.630 kb on - strand, within Dshi_2528 at 2689.685 kb on + strand, within Dshi_2528 at 2689.699 kb on - strand, within Dshi_2528 at 2689.722 kb on + strand, within Dshi_2528 at 2689.737 kb on - strand, within Dshi_2528 at 2689.737 kb on - strand, within Dshi_2528 at 2689.786 kb on - strand, within Dshi_2528 at 2689.787 kb on + strand, within Dshi_2528 at 2689.795 kb on - strand, within Dshi_2528 at 2689.800 kb on - strand, within Dshi_2528 at 2689.849 kb on + strand, within Dshi_2528 at 2689.882 kb on - strand, within Dshi_2528 at 2690.087 kb on + strand, within Dshi_2529 at 2690.100 kb on - strand, within Dshi_2529 at 2690.102 kb on + strand, within Dshi_2529 at 2690.107 kb on + strand, within Dshi_2529
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 5 vol% remove 2,687,739 + Dshi_2526 0.58 -1.1 2,687,780 - Dshi_2526 0.61 +2.7 2,687,780 - Dshi_2526 0.61 -0.2 2,687,832 + Dshi_2526 0.65 +0.9 2,687,937 + Dshi_2526 0.73 +0.3 2,687,945 - Dshi_2526 0.74 -0.9 2,687,988 - Dshi_2526 0.77 +1.0 2,688,000 - Dshi_2526 0.78 -1.6 2,688,011 + Dshi_2526 0.79 -0.4 2,688,064 - Dshi_2526 0.83 +0.0 2,688,074 - Dshi_2526 0.83 +0.3 2,688,079 - Dshi_2526 0.84 -0.4 2,688,158 - Dshi_2526 0.90 +0.3 2,688,201 + -0.1 2,688,201 + +0.6 2,688,209 - +0.7 2,688,209 - -0.0 2,688,209 - -1.3 2,688,248 + -0.2 2,688,318 + +0.1 2,688,326 + -0.1 2,688,326 + +1.1 2,688,334 - -0.9 2,688,334 - -1.1 2,688,334 - +0.9 2,688,384 - -0.6 2,688,394 + -0.5 2,688,395 + +0.8 2,688,408 - -0.8 2,688,440 - -0.1 2,688,440 - +0.1 2,688,450 - -1.7 2,688,450 - +0.4 2,688,599 + -0.4 2,688,607 - -0.5 2,688,702 - -0.1 2,688,703 + +0.4 2,688,764 - +0.4 2,688,764 - -0.6 2,688,800 - Dshi_2527 0.18 -0.6 2,688,805 - Dshi_2527 0.19 -0.7 2,688,805 - Dshi_2527 0.19 +0.0 2,688,870 - Dshi_2527 0.35 -0.3 2,688,920 - Dshi_2527 0.48 +0.7 2,688,922 + Dshi_2527 0.49 +0.0 2,688,956 - Dshi_2527 0.57 +1.0 2,689,009 + Dshi_2527 0.71 +0.6 2,689,015 + Dshi_2527 0.72 -0.1 2,689,070 + Dshi_2527 0.86 -0.2 2,689,086 - +0.8 2,689,183 + -0.2 2,689,204 + +0.4 2,689,222 - +0.4 2,689,260 + +0.8 2,689,273 - -0.4 2,689,280 + +1.3 2,689,324 + Dshi_2528 0.14 -1.3 2,689,356 + Dshi_2528 0.19 -0.3 2,689,396 + Dshi_2528 0.24 -0.1 2,689,453 - Dshi_2528 0.31 -0.6 2,689,626 - Dshi_2528 0.54 -0.2 2,689,630 - Dshi_2528 0.55 -0.3 2,689,685 + Dshi_2528 0.62 -0.8 2,689,699 - Dshi_2528 0.64 -1.0 2,689,722 + Dshi_2528 0.67 +0.5 2,689,737 - Dshi_2528 0.69 -0.5 2,689,737 - Dshi_2528 0.69 -1.6 2,689,786 - Dshi_2528 0.75 -0.1 2,689,787 + Dshi_2528 0.76 -0.4 2,689,795 - Dshi_2528 0.77 -0.1 2,689,800 - Dshi_2528 0.77 +0.9 2,689,849 + Dshi_2528 0.84 -0.6 2,689,882 - Dshi_2528 0.88 -1.1 2,690,087 + Dshi_2529 0.11 -0.4 2,690,100 - Dshi_2529 0.12 +0.8 2,690,102 + Dshi_2529 0.13 +1.5 2,690,107 + Dshi_2529 0.13 -0.4
Or see this region's nucleotide sequence