Experiment: m.b. Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2048 and Dshi_2049 are separated by 21 nucleotides Dshi_2049 and Dshi_2050 overlap by 1 nucleotides Dshi_2050 and Dshi_2051 are separated by 105 nucleotides Dshi_2051 and Dshi_2052 overlap by 4 nucleotides
Dshi_2048: Dshi_2048 - Mammalian cell entry related domain protein (RefSeq), at 2,167,769 to 2,169,508
_2048
Dshi_2049: Dshi_2049 - ABC transporter related (RefSeq), at 2,169,530 to 2,170,309
_2049
Dshi_2050: Dshi_2050 - protein of unknown function DUF140 (RefSeq), at 2,170,309 to 2,171,463
_2050
Dshi_2051: Dshi_2051 - hypothetical protein (RefSeq), at 2,171,569 to 2,172,045
_2051
Dshi_2052: Dshi_2052 - hypothetical protein (RefSeq), at 2,172,042 to 2,172,206
_2052
Position (kb)
2170
2171
2172 Strain fitness (log2 ratio)
-1
0
1
2 at 2169.356 kb on + strand at 2169.370 kb on - strand at 2169.409 kb on - strand at 2169.411 kb on + strand at 2169.419 kb on - strand at 2169.419 kb on - strand at 2169.563 kb on - strand at 2169.596 kb on - strand at 2169.624 kb on - strand, within Dshi_2049 at 2169.687 kb on + strand, within Dshi_2049 at 2169.717 kb on - strand, within Dshi_2049 at 2169.749 kb on - strand, within Dshi_2049 at 2169.870 kb on - strand, within Dshi_2049 at 2169.958 kb on + strand, within Dshi_2049 at 2169.980 kb on + strand, within Dshi_2049 at 2169.980 kb on + strand, within Dshi_2049 at 2169.988 kb on - strand, within Dshi_2049 at 2170.014 kb on - strand, within Dshi_2049 at 2170.093 kb on + strand, within Dshi_2049 at 2170.204 kb on - strand, within Dshi_2049 at 2170.386 kb on - strand at 2170.433 kb on - strand, within Dshi_2050 at 2170.457 kb on + strand, within Dshi_2050 at 2170.490 kb on + strand, within Dshi_2050 at 2170.490 kb on + strand, within Dshi_2050 at 2170.591 kb on - strand, within Dshi_2050 at 2170.636 kb on + strand, within Dshi_2050 at 2170.758 kb on - strand, within Dshi_2050 at 2170.793 kb on + strand, within Dshi_2050 at 2170.924 kb on + strand, within Dshi_2050 at 2170.931 kb on + strand, within Dshi_2050 at 2170.931 kb on + strand, within Dshi_2050 at 2171.007 kb on - strand, within Dshi_2050 at 2171.017 kb on - strand, within Dshi_2050 at 2171.017 kb on - strand, within Dshi_2050 at 2171.073 kb on - strand, within Dshi_2050 at 2171.119 kb on - strand, within Dshi_2050 at 2171.240 kb on + strand, within Dshi_2050 at 2171.272 kb on - strand, within Dshi_2050 at 2171.274 kb on + strand, within Dshi_2050 at 2171.479 kb on - strand at 2171.479 kb on - strand at 2171.503 kb on - strand at 2171.508 kb on - strand at 2171.532 kb on - strand at 2171.541 kb on + strand at 2171.549 kb on - strand at 2171.739 kb on - strand, within Dshi_2051 at 2171.762 kb on + strand, within Dshi_2051 at 2171.775 kb on - strand, within Dshi_2051 at 2171.798 kb on + strand, within Dshi_2051 at 2171.802 kb on - strand, within Dshi_2051 at 2171.843 kb on - strand, within Dshi_2051 at 2171.863 kb on + strand, within Dshi_2051 at 2171.889 kb on + strand, within Dshi_2051 at 2171.891 kb on - strand, within Dshi_2051 at 2171.912 kb on + strand, within Dshi_2051 at 2172.085 kb on + strand, within Dshi_2052 at 2172.103 kb on + strand, within Dshi_2052 at 2172.204 kb on - strand at 2172.211 kb on - strand at 2172.262 kb on + strand at 2172.317 kb on + strand at 2172.335 kb on - strand at 2172.368 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 5 vol% remove 2,169,356 + -0.5 2,169,370 - -0.2 2,169,409 - +1.5 2,169,411 + +0.6 2,169,419 - -0.1 2,169,419 - +0.3 2,169,563 - +0.2 2,169,596 - -1.6 2,169,624 - Dshi_2049 0.12 +0.6 2,169,687 + Dshi_2049 0.20 -0.0 2,169,717 - Dshi_2049 0.24 -0.1 2,169,749 - Dshi_2049 0.28 -0.7 2,169,870 - Dshi_2049 0.44 -0.8 2,169,958 + Dshi_2049 0.55 -1.3 2,169,980 + Dshi_2049 0.58 +1.2 2,169,980 + Dshi_2049 0.58 -0.3 2,169,988 - Dshi_2049 0.59 -0.0 2,170,014 - Dshi_2049 0.62 +0.9 2,170,093 + Dshi_2049 0.72 +1.0 2,170,204 - Dshi_2049 0.86 -0.3 2,170,386 - -0.0 2,170,433 - Dshi_2050 0.11 -0.3 2,170,457 + Dshi_2050 0.13 +0.3 2,170,490 + Dshi_2050 0.16 +0.4 2,170,490 + Dshi_2050 0.16 +2.5 2,170,591 - Dshi_2050 0.24 +0.4 2,170,636 + Dshi_2050 0.28 +0.6 2,170,758 - Dshi_2050 0.39 +0.4 2,170,793 + Dshi_2050 0.42 +0.2 2,170,924 + Dshi_2050 0.53 +0.2 2,170,931 + Dshi_2050 0.54 -0.5 2,170,931 + Dshi_2050 0.54 +0.2 2,171,007 - Dshi_2050 0.60 -0.0 2,171,017 - Dshi_2050 0.61 +0.9 2,171,017 - Dshi_2050 0.61 +0.1 2,171,073 - Dshi_2050 0.66 -0.6 2,171,119 - Dshi_2050 0.70 +0.1 2,171,240 + Dshi_2050 0.81 -0.8 2,171,272 - Dshi_2050 0.83 -0.1 2,171,274 + Dshi_2050 0.84 +0.6 2,171,479 - -0.6 2,171,479 - +0.3 2,171,503 - -0.2 2,171,508 - -0.7 2,171,532 - -0.0 2,171,541 + +0.4 2,171,549 - +0.9 2,171,739 - Dshi_2051 0.36 +0.4 2,171,762 + Dshi_2051 0.40 +0.2 2,171,775 - Dshi_2051 0.43 +0.2 2,171,798 + Dshi_2051 0.48 +0.4 2,171,802 - Dshi_2051 0.49 -0.6 2,171,843 - Dshi_2051 0.57 +0.3 2,171,863 + Dshi_2051 0.62 -0.8 2,171,889 + Dshi_2051 0.67 -0.2 2,171,891 - Dshi_2051 0.68 +0.5 2,171,912 + Dshi_2051 0.72 -0.1 2,172,085 + Dshi_2052 0.26 +0.3 2,172,103 + Dshi_2052 0.37 +0.7 2,172,204 - +0.2 2,172,211 - +2.2 2,172,262 + -0.2 2,172,317 + +0.3 2,172,335 - -0.4 2,172,368 - +1.0
Or see this region's nucleotide sequence