Experiment: BHIS with Irgasan 0.005 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2617 and BT2618 are separated by 466 nucleotides BT2618 and BT2619 are separated by 4 nucleotides
BT2617: BT2617 - reverse transcriptase (NCBI ptt file), at 3,256,696 to 3,258,102
BT2617
BT2618: BT2618 - two-component system response regulator (NCBI ptt file), at 3,258,569 to 3,259,849
BT2618
BT2619: BT2619 - two-component system sensor histidine kinase (NCBI ptt file), at 3,259,854 to 3,262,226
BT2619
Position (kb)
3258
3259
3260 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3257.569 kb on - strand, within BT2617 at 3257.569 kb on - strand, within BT2617 at 3257.569 kb on - strand, within BT2617 at 3257.570 kb on + strand, within BT2617 at 3257.571 kb on - strand, within BT2617 at 3257.571 kb on - strand, within BT2617 at 3257.667 kb on - strand, within BT2617 at 3257.667 kb on - strand, within BT2617 at 3257.761 kb on + strand, within BT2617 at 3257.815 kb on + strand, within BT2617 at 3257.839 kb on + strand, within BT2617 at 3257.852 kb on - strand, within BT2617 at 3257.852 kb on - strand, within BT2617 at 3257.852 kb on - strand, within BT2617 at 3257.912 kb on + strand, within BT2617 at 3257.937 kb on + strand, within BT2617 at 3257.938 kb on - strand, within BT2617 at 3257.953 kb on + strand, within BT2617 at 3257.975 kb on - strand at 3258.045 kb on + strand at 3258.128 kb on + strand at 3258.173 kb on - strand at 3258.175 kb on + strand at 3258.176 kb on - strand at 3258.240 kb on + strand at 3258.269 kb on + strand at 3258.270 kb on - strand at 3258.285 kb on + strand at 3258.286 kb on - strand at 3258.319 kb on + strand at 3258.319 kb on + strand at 3258.324 kb on - strand at 3258.324 kb on - strand at 3258.324 kb on - strand at 3258.326 kb on - strand at 3258.370 kb on + strand at 3258.370 kb on + strand at 3258.371 kb on - strand at 3258.371 kb on - strand at 3258.372 kb on + strand at 3258.373 kb on - strand at 3258.399 kb on + strand at 3258.449 kb on + strand at 3258.449 kb on + strand at 3258.458 kb on + strand at 3258.458 kb on + strand at 3258.459 kb on - strand at 3258.504 kb on + strand at 3258.504 kb on + strand at 3258.505 kb on - strand at 3258.505 kb on - strand at 3258.530 kb on + strand at 3258.556 kb on + strand at 3258.577 kb on + strand at 3258.583 kb on - strand at 3258.600 kb on + strand at 3258.600 kb on + strand at 3258.678 kb on - strand at 3258.800 kb on - strand, within BT2618 at 3258.933 kb on + strand, within BT2618 at 3259.145 kb on + strand, within BT2618 at 3259.184 kb on - strand, within BT2618 at 3259.184 kb on - strand, within BT2618 at 3259.357 kb on + strand, within BT2618 at 3259.357 kb on + strand, within BT2618 at 3259.357 kb on + strand, within BT2618 at 3259.358 kb on - strand, within BT2618 at 3259.434 kb on + strand, within BT2618 at 3259.467 kb on + strand, within BT2618 at 3259.615 kb on + strand, within BT2618 at 3259.615 kb on + strand, within BT2618 at 3259.636 kb on - strand, within BT2618 at 3259.669 kb on + strand, within BT2618 at 3259.669 kb on + strand, within BT2618 at 3259.754 kb on - strand at 3259.829 kb on + strand at 3259.832 kb on - strand at 3259.832 kb on - strand at 3259.921 kb on + strand at 3259.921 kb on + strand at 3259.922 kb on - strand at 3259.922 kb on - strand at 3259.925 kb on + strand at 3259.926 kb on - strand at 3259.926 kb on - strand at 3259.968 kb on + strand at 3259.973 kb on + strand at 3260.252 kb on + strand, within BT2619 at 3260.319 kb on + strand, within BT2619 at 3260.425 kb on + strand, within BT2619 at 3260.470 kb on + strand, within BT2619 at 3260.470 kb on + strand, within BT2619 at 3260.471 kb on - strand, within BT2619 at 3260.565 kb on + strand, within BT2619 at 3260.572 kb on + strand, within BT2619 at 3260.572 kb on + strand, within BT2619 at 3260.572 kb on + strand, within BT2619 at 3260.590 kb on - strand, within BT2619 at 3260.609 kb on + strand, within BT2619 at 3260.643 kb on - strand, within BT2619 at 3260.778 kb on + strand, within BT2619 at 3260.819 kb on + strand, within BT2619 at 3260.834 kb on + strand, within BT2619 at 3260.834 kb on + strand, within BT2619
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.005 mM remove 3,257,569 - BT2617 0.62 -0.9 3,257,569 - BT2617 0.62 -1.1 3,257,569 - BT2617 0.62 -0.1 3,257,570 + BT2617 0.62 -0.9 3,257,571 - BT2617 0.62 -0.4 3,257,571 - BT2617 0.62 +1.0 3,257,667 - BT2617 0.69 -1.3 3,257,667 - BT2617 0.69 -1.2 3,257,761 + BT2617 0.76 +0.2 3,257,815 + BT2617 0.80 -0.9 3,257,839 + BT2617 0.81 +0.3 3,257,852 - BT2617 0.82 -1.3 3,257,852 - BT2617 0.82 -0.5 3,257,852 - BT2617 0.82 -0.4 3,257,912 + BT2617 0.86 -0.6 3,257,937 + BT2617 0.88 -0.6 3,257,938 - BT2617 0.88 +0.4 3,257,953 + BT2617 0.89 +1.3 3,257,975 - -0.0 3,258,045 + -0.0 3,258,128 + -1.3 3,258,173 - -0.8 3,258,175 + -0.3 3,258,176 - +0.4 3,258,240 + +1.3 3,258,269 + -1.5 3,258,270 - -1.8 3,258,285 + -1.8 3,258,286 - +0.6 3,258,319 + -0.6 3,258,319 + -0.9 3,258,324 - +0.3 3,258,324 - -0.6 3,258,324 - -0.8 3,258,326 - -0.6 3,258,370 + -0.9 3,258,370 + +1.0 3,258,371 - -0.8 3,258,371 - -1.0 3,258,372 + -0.6 3,258,373 - -1.0 3,258,399 + -0.9 3,258,449 + -2.1 3,258,449 + -0.1 3,258,458 + -1.7 3,258,458 + -1.1 3,258,459 - -1.8 3,258,504 + +0.0 3,258,504 + +1.0 3,258,505 - -1.9 3,258,505 - -2.1 3,258,530 + -0.6 3,258,556 + +0.5 3,258,577 + -1.9 3,258,583 - +0.0 3,258,600 + +0.0 3,258,600 + -1.1 3,258,678 - -0.7 3,258,800 - BT2618 0.18 -1.6 3,258,933 + BT2618 0.28 +0.0 3,259,145 + BT2618 0.45 +0.0 3,259,184 - BT2618 0.48 -1.8 3,259,184 - BT2618 0.48 -2.1 3,259,357 + BT2618 0.62 +2.0 3,259,357 + BT2618 0.62 -0.4 3,259,357 + BT2618 0.62 -1.1 3,259,358 - BT2618 0.62 -1.3 3,259,434 + BT2618 0.68 +1.0 3,259,467 + BT2618 0.70 +0.0 3,259,615 + BT2618 0.82 -1.0 3,259,615 + BT2618 0.82 -1.0 3,259,636 - BT2618 0.83 +0.4 3,259,669 + BT2618 0.86 -1.2 3,259,669 + BT2618 0.86 -1.5 3,259,754 - -1.9 3,259,829 + -1.9 3,259,832 - -2.5 3,259,832 - -1.9 3,259,921 + +1.0 3,259,921 + -1.7 3,259,922 - -2.2 3,259,922 - -1.8 3,259,925 + -1.0 3,259,926 - -0.4 3,259,926 - +0.3 3,259,968 + +2.0 3,259,973 + -1.0 3,260,252 + BT2619 0.17 -0.1 3,260,319 + BT2619 0.20 -1.4 3,260,425 + BT2619 0.24 -2.0 3,260,470 + BT2619 0.26 -1.1 3,260,470 + BT2619 0.26 -0.2 3,260,471 - BT2619 0.26 -0.7 3,260,565 + BT2619 0.30 -0.1 3,260,572 + BT2619 0.30 -1.3 3,260,572 + BT2619 0.30 -2.0 3,260,572 + BT2619 0.30 +0.2 3,260,590 - BT2619 0.31 -0.6 3,260,609 + BT2619 0.32 -0.2 3,260,643 - BT2619 0.33 -1.6 3,260,778 + BT2619 0.39 -0.9 3,260,819 + BT2619 0.41 +2.0 3,260,834 + BT2619 0.41 +1.0 3,260,834 + BT2619 0.41 -1.2
Or see this region's nucleotide sequence