Experiment: BHIS with Irgasan 0.005 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1129 and BT1130 are separated by 43 nucleotides BT1130 and BT1131 are separated by 195 nucleotides BT1131 and BT1132 overlap by 28 nucleotides BT1132 and BT1133 are separated by 145 nucleotides BT1133 and BT1134 are separated by 237 nucleotides
BT1129: BT1129 - conserved hypothetical protein (NCBI ptt file), at 1,417,749 to 1,418,060
BT1129
BT1130: BT1130 - hypothetical protein (NCBI ptt file), at 1,418,104 to 1,418,427
BT1130
BT1131: BT1131 - hypothetical protein (NCBI ptt file), at 1,418,623 to 1,418,808
BT1131
BT1132: BT1132 - hypothetical protein (NCBI ptt file), at 1,418,781 to 1,419,230
BT1132
BT1133: BT1133 - hypothetical protein (NCBI ptt file), at 1,419,376 to 1,419,936
BT1133
BT1134: BT1134 - hypothetical protein (NCBI ptt file), at 1,420,174 to 1,420,554
BT1134
Position (kb)
1418
1419
1420 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1417.977 kb on + strand, within BT1129 at 1418.000 kb on + strand, within BT1129 at 1418.153 kb on + strand, within BT1130 at 1418.153 kb on + strand, within BT1130 at 1418.539 kb on - strand at 1418.559 kb on + strand at 1418.590 kb on + strand at 1418.591 kb on - strand at 1418.636 kb on - strand at 1418.670 kb on + strand, within BT1131 at 1418.673 kb on + strand, within BT1131 at 1418.812 kb on + strand at 1418.812 kb on + strand at 1418.845 kb on + strand, within BT1132 at 1418.922 kb on + strand, within BT1132 at 1418.923 kb on - strand, within BT1132 at 1418.923 kb on - strand, within BT1132 at 1418.924 kb on + strand, within BT1132 at 1418.925 kb on - strand, within BT1132 at 1418.954 kb on - strand, within BT1132 at 1418.980 kb on - strand, within BT1132 at 1418.986 kb on + strand, within BT1132 at 1419.292 kb on + strand at 1419.292 kb on + strand at 1419.292 kb on + strand at 1419.293 kb on - strand at 1419.328 kb on + strand at 1419.328 kb on + strand at 1419.329 kb on - strand at 1419.354 kb on - strand at 1419.533 kb on + strand, within BT1133 at 1419.533 kb on + strand, within BT1133 at 1419.604 kb on + strand, within BT1133 at 1419.605 kb on - strand, within BT1133 at 1419.738 kb on - strand, within BT1133 at 1419.813 kb on - strand, within BT1133 at 1419.879 kb on - strand, within BT1133 at 1419.936 kb on + strand at 1419.937 kb on - strand at 1419.958 kb on - strand at 1419.959 kb on + strand at 1420.179 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Irgasan 0.005 mM remove 1,417,977 + BT1129 0.73 -0.9 1,418,000 + BT1129 0.80 -3.2 1,418,153 + BT1130 0.15 -0.7 1,418,153 + BT1130 0.15 -1.9 1,418,539 - -1.0 1,418,559 + -0.6 1,418,590 + -1.2 1,418,591 - -0.1 1,418,636 - +0.3 1,418,670 + BT1131 0.25 +1.0 1,418,673 + BT1131 0.27 -0.0 1,418,812 + -0.6 1,418,812 + -1.1 1,418,845 + BT1132 0.14 -2.0 1,418,922 + BT1132 0.31 -1.1 1,418,923 - BT1132 0.32 -1.5 1,418,923 - BT1132 0.32 -0.6 1,418,924 + BT1132 0.32 -1.7 1,418,925 - BT1132 0.32 -0.6 1,418,954 - BT1132 0.38 -1.7 1,418,980 - BT1132 0.44 -1.3 1,418,986 + BT1132 0.46 -0.3 1,419,292 + -1.3 1,419,292 + -1.1 1,419,292 + -1.2 1,419,293 - -0.7 1,419,328 + -0.9 1,419,328 + -1.0 1,419,329 - -0.9 1,419,354 - -0.0 1,419,533 + BT1133 0.28 -0.0 1,419,533 + BT1133 0.28 +1.6 1,419,604 + BT1133 0.41 -0.7 1,419,605 - BT1133 0.41 -0.6 1,419,738 - BT1133 0.65 +1.0 1,419,813 - BT1133 0.78 -2.2 1,419,879 - BT1133 0.90 -0.3 1,419,936 + -0.5 1,419,937 - +2.0 1,419,958 - +0.7 1,419,959 + +1.0 1,420,179 + -1.2
Or see this region's nucleotide sequence