Experiment: formate McAA biotin dropout
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00685 and MMP_RS00690 are separated by 46 nucleotides MMP_RS00690 and MMP_RS00695 are separated by 12 nucleotides MMP_RS00695 and MMP_RS00700 are separated by 11 nucleotides MMP_RS00700 and MMP_RS00705 are separated by 12 nucleotides MMP_RS00705 and MMP_RS00710 are separated by 3 nucleotides
MMP_RS00685: MMP_RS00685 - 2-phospho-L-lactate guanylyltransferase, at 125,209 to 125,877
_RS00685
MMP_RS00690: MMP_RS00690 - MBL fold metallo-hydrolase, at 125,924 to 126,541
_RS00690
MMP_RS00695: MMP_RS00695 - biotin--[acetyl-CoA-carboxylase] ligase, at 126,554 to 127,249
_RS00695
MMP_RS00700: MMP_RS00700 - OB-fold nucleic acid binding domain-containing protein, at 127,261 to 127,647
_RS00700
MMP_RS00705: MMP_RS00705 - hypothetical protein, at 127,660 to 127,977
_RS00705
MMP_RS00710: MMP_RS00710 - hypothetical protein, at 127,981 to 128,889
_RS00710
Position (kb) 126
127
128 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 125.785 kb on - strand, within MMP_RS00685 at 125.906 kb on - strand at 125.925 kb on - strand at 125.930 kb on + strand at 125.958 kb on + strand at 125.988 kb on + strand, within MMP_RS00690 at 126.008 kb on + strand, within MMP_RS00690 at 126.009 kb on - strand, within MMP_RS00690 at 126.131 kb on - strand, within MMP_RS00690 at 126.170 kb on - strand, within MMP_RS00690 at 126.263 kb on + strand, within MMP_RS00690 at 126.271 kb on - strand, within MMP_RS00690 at 126.280 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.305 kb on - strand, within MMP_RS00690 at 126.318 kb on + strand, within MMP_RS00690 at 126.391 kb on - strand, within MMP_RS00690 at 126.391 kb on - strand, within MMP_RS00690 at 126.396 kb on - strand, within MMP_RS00690 at 126.491 kb on - strand at 126.671 kb on - strand, within MMP_RS00695 at 126.821 kb on - strand, within MMP_RS00695 at 127.200 kb on - strand at 127.262 kb on - strand at 127.276 kb on - strand at 127.283 kb on + strand at 127.327 kb on - strand, within MMP_RS00700 at 127.332 kb on + strand, within MMP_RS00700 at 127.356 kb on - strand, within MMP_RS00700 at 127.496 kb on - strand, within MMP_RS00700 at 127.517 kb on - strand, within MMP_RS00700 at 127.563 kb on + strand, within MMP_RS00700 at 127.831 kb on + strand, within MMP_RS00705 at 127.841 kb on + strand, within MMP_RS00705 at 128.064 kb on + strand at 128.205 kb on - strand, within MMP_RS00710
Per-strain Table
Position Strand Gene LocusTag Fraction formate McAA biotin dropout remove 125,785 - MMP_RS00685 0.86 -3.0 125,906 - -0.8 125,925 - -0.2 125,930 + +0.1 125,958 + +0.1 125,988 + MMP_RS00690 0.10 -1.1 126,008 + MMP_RS00690 0.14 +0.3 126,009 - MMP_RS00690 0.14 +0.2 126,131 - MMP_RS00690 0.33 +0.6 126,170 - MMP_RS00690 0.40 -0.8 126,263 + MMP_RS00690 0.55 +0.5 126,271 - MMP_RS00690 0.56 +0.5 126,280 - MMP_RS00690 0.58 -0.4 126,305 - MMP_RS00690 0.62 +0.3 126,305 - MMP_RS00690 0.62 +0.1 126,305 - MMP_RS00690 0.62 +0.4 126,318 + MMP_RS00690 0.64 -0.8 126,391 - MMP_RS00690 0.76 -0.5 126,391 - MMP_RS00690 0.76 -0.1 126,396 - MMP_RS00690 0.76 -0.1 126,491 - +0.5 126,671 - MMP_RS00695 0.17 -3.8 126,821 - MMP_RS00695 0.38 -2.8 127,200 - -1.8 127,262 - -1.3 127,276 - +0.2 127,283 + -0.3 127,327 - MMP_RS00700 0.17 -0.4 127,332 + MMP_RS00700 0.18 -0.1 127,356 - MMP_RS00700 0.25 +0.0 127,496 - MMP_RS00700 0.61 -0.0 127,517 - MMP_RS00700 0.66 +0.4 127,563 + MMP_RS00700 0.78 -0.1 127,831 + MMP_RS00705 0.54 +0.3 127,841 + MMP_RS00705 0.57 -0.3 128,064 + -0.4 128,205 - MMP_RS00710 0.25 +0.1
Or see this region's nucleotide sequence