Experiment: m.b. Fusidic 0.0006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2378 and Dshi_2379 overlap by 4 nucleotides Dshi_2379 and Dshi_2380 overlap by 4 nucleotides Dshi_2380 and Dshi_R0044 are separated by 88 nucleotides Dshi_R0044 and Dshi_2381 are separated by 190 nucleotides
Dshi_2378: Dshi_2378 - alanine racemase (RefSeq), at 2,512,525 to 2,513,574
_2378
Dshi_2379: Dshi_2379 - protein of unknown function DUF140 (RefSeq), at 2,513,571 to 2,514,347
_2379
Dshi_2380: Dshi_2380 - ABC transporter related (RefSeq), at 2,514,344 to 2,515,117
_2380
Dshi_R0044: Dshi_R0044 - tRNA-Leu (RefSeq), at 2,515,206 to 2,515,292
_R0044
Dshi_2381: Dshi_2381 - lipoate-protein ligase B (RefSeq), at 2,515,483 to 2,516,145
_2381
Position (kb)
2514
2515
2516 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2513.365 kb on + strand, within Dshi_2378 at 2513.662 kb on + strand, within Dshi_2379 at 2513.687 kb on - strand, within Dshi_2379 at 2513.733 kb on - strand, within Dshi_2379 at 2513.871 kb on + strand, within Dshi_2379 at 2513.913 kb on - strand, within Dshi_2379 at 2514.001 kb on + strand, within Dshi_2379 at 2514.041 kb on + strand, within Dshi_2379 at 2514.233 kb on - strand, within Dshi_2379 at 2514.251 kb on + strand, within Dshi_2379 at 2514.251 kb on + strand, within Dshi_2379 at 2514.251 kb on + strand, within Dshi_2379 at 2514.571 kb on + strand, within Dshi_2380 at 2514.740 kb on + strand, within Dshi_2380 at 2514.784 kb on - strand, within Dshi_2380 at 2514.841 kb on - strand, within Dshi_2380 at 2514.867 kb on + strand, within Dshi_2380 at 2514.907 kb on - strand, within Dshi_2380 at 2515.459 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0006 mg/ml remove 2,513,365 + Dshi_2378 0.80 -2.8 2,513,662 + Dshi_2379 0.12 -2.9 2,513,687 - Dshi_2379 0.15 -2.1 2,513,733 - Dshi_2379 0.21 -1.6 2,513,871 + Dshi_2379 0.39 -0.1 2,513,913 - Dshi_2379 0.44 -4.0 2,514,001 + Dshi_2379 0.55 -3.5 2,514,041 + Dshi_2379 0.60 -0.1 2,514,233 - Dshi_2379 0.85 -2.1 2,514,251 + Dshi_2379 0.88 +0.4 2,514,251 + Dshi_2379 0.88 -2.1 2,514,251 + Dshi_2379 0.88 -1.9 2,514,571 + Dshi_2380 0.29 -3.2 2,514,740 + Dshi_2380 0.51 -3.7 2,514,784 - Dshi_2380 0.57 -4.3 2,514,841 - Dshi_2380 0.64 -0.6 2,514,867 + Dshi_2380 0.68 -0.6 2,514,907 - Dshi_2380 0.73 -3.8 2,515,459 + -0.6
Or see this region's nucleotide sequence