Experiment: BHIS with Nitrofurantoin 0.15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3430 and tRNA-Glu are separated by 39 nucleotides tRNA-Glu and BT3431 are separated by 605 nucleotides BT3431 and BT3432 are separated by 4 nucleotides
BT3430: BT3430 - ribosomal protein S20 (NCBI ptt file), at 4,420,476 to 4,420,730
BT3430
BT_t55: tRNA-Glu - tRNA-Glu (RefSeq), at 4,420,770 to 4,420,844
tRNA-Glu
BT3431: BT3431 - DNA repair protein (NCBI ptt file), at 4,421,450 to 4,422,178
BT3431
BT3432: BT3432 - conserved hypothetical protein (NCBI ptt file), at 4,422,183 to 4,423,922
BT3432
Position (kb)
4421
4422
4423 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4420.451 kb on - strand at 4420.451 kb on - strand at 4420.477 kb on + strand at 4421.056 kb on + strand at 4421.063 kb on - strand at 4421.069 kb on - strand at 4421.069 kb on - strand at 4421.082 kb on + strand at 4421.093 kb on - strand at 4421.093 kb on - strand at 4421.097 kb on - strand at 4421.112 kb on + strand at 4421.115 kb on - strand at 4421.136 kb on + strand at 4421.146 kb on - strand at 4421.148 kb on - strand at 4421.148 kb on - strand at 4421.151 kb on + strand at 4421.153 kb on + strand at 4421.164 kb on - strand at 4421.166 kb on + strand at 4421.188 kb on + strand at 4421.189 kb on - strand at 4421.190 kb on + strand at 4421.259 kb on + strand at 4421.260 kb on - strand at 4421.308 kb on + strand at 4421.309 kb on - strand at 4421.325 kb on - strand at 4421.342 kb on + strand at 4421.342 kb on + strand at 4421.342 kb on + strand at 4421.342 kb on + strand at 4421.342 kb on + strand at 4421.353 kb on + strand at 4421.449 kb on + strand at 4421.449 kb on + strand at 4421.478 kb on + strand at 4421.479 kb on - strand at 4421.479 kb on - strand at 4421.482 kb on + strand at 4421.482 kb on + strand at 4421.483 kb on - strand at 4421.489 kb on - strand at 4421.494 kb on + strand at 4421.501 kb on - strand at 4421.514 kb on + strand at 4421.514 kb on + strand at 4421.515 kb on - strand at 4421.515 kb on - strand at 4421.519 kb on - strand at 4421.519 kb on - strand at 4421.523 kb on - strand, within BT3431 at 4421.692 kb on + strand, within BT3431 at 4421.692 kb on + strand, within BT3431 at 4421.714 kb on + strand, within BT3431 at 4421.714 kb on + strand, within BT3431 at 4421.715 kb on - strand, within BT3431 at 4421.715 kb on - strand, within BT3431 at 4421.715 kb on - strand, within BT3431 at 4421.754 kb on - strand, within BT3431 at 4421.962 kb on + strand, within BT3431 at 4422.141 kb on + strand at 4422.141 kb on + strand at 4422.157 kb on - strand at 4422.157 kb on - strand at 4422.186 kb on + strand at 4422.226 kb on - strand at 4422.240 kb on + strand at 4422.376 kb on + strand, within BT3432 at 4422.377 kb on - strand, within BT3432 at 4422.383 kb on - strand, within BT3432 at 4422.467 kb on + strand, within BT3432 at 4422.469 kb on + strand, within BT3432 at 4422.469 kb on + strand, within BT3432 at 4422.473 kb on - strand, within BT3432 at 4422.515 kb on - strand, within BT3432 at 4422.604 kb on + strand, within BT3432 at 4422.635 kb on + strand, within BT3432 at 4422.636 kb on - strand, within BT3432 at 4422.649 kb on + strand, within BT3432 at 4422.651 kb on + strand, within BT3432 at 4422.671 kb on - strand, within BT3432 at 4422.685 kb on + strand, within BT3432 at 4422.779 kb on - strand, within BT3432 at 4422.852 kb on - strand, within BT3432 at 4422.876 kb on + strand, within BT3432 at 4422.876 kb on + strand, within BT3432 at 4422.966 kb on - strand, within BT3432 at 4422.995 kb on - strand, within BT3432 at 4423.010 kb on - strand, within BT3432 at 4423.030 kb on + strand, within BT3432 at 4423.030 kb on + strand, within BT3432 at 4423.031 kb on - strand, within BT3432 at 4423.069 kb on + strand, within BT3432 at 4423.099 kb on - strand, within BT3432 at 4423.101 kb on + strand, within BT3432 at 4423.102 kb on - strand, within BT3432 at 4423.113 kb on - strand, within BT3432 at 4423.161 kb on + strand, within BT3432
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Nitrofurantoin 0.15 mM remove 4,420,451 - -0.8 4,420,451 - +1.0 4,420,477 + -1.6 4,421,056 + -1.0 4,421,063 - -2.9 4,421,069 - -2.2 4,421,069 - -1.6 4,421,082 + -0.0 4,421,093 - -2.2 4,421,093 - +2.0 4,421,097 - -1.3 4,421,112 + -0.0 4,421,115 - -1.6 4,421,136 + -0.0 4,421,146 - -2.7 4,421,148 - -3.0 4,421,148 - -1.7 4,421,151 + -0.6 4,421,153 + -0.0 4,421,164 - -1.4 4,421,166 + +1.0 4,421,188 + -1.1 4,421,189 - -2.6 4,421,190 + +1.0 4,421,259 + -1.6 4,421,260 - -1.6 4,421,308 + -0.0 4,421,309 - -1.3 4,421,325 - -2.4 4,421,342 + +1.6 4,421,342 + +1.0 4,421,342 + +1.0 4,421,342 + -1.3 4,421,342 + -0.0 4,421,353 + -0.8 4,421,449 + -1.0 4,421,449 + -0.7 4,421,478 + -3.4 4,421,479 - -2.7 4,421,479 - -5.0 4,421,482 + -2.7 4,421,482 + -2.9 4,421,483 - -2.0 4,421,489 - -0.0 4,421,494 + -1.6 4,421,501 - -5.6 4,421,514 + -2.2 4,421,514 + -0.0 4,421,515 - -2.8 4,421,515 - -3.9 4,421,519 - -3.0 4,421,519 - -1.3 4,421,523 - BT3431 0.10 -4.0 4,421,692 + BT3431 0.33 -3.0 4,421,692 + BT3431 0.33 -3.5 4,421,714 + BT3431 0.36 -3.5 4,421,714 + BT3431 0.36 -3.9 4,421,715 - BT3431 0.36 -1.8 4,421,715 - BT3431 0.36 +1.0 4,421,715 - BT3431 0.36 -4.2 4,421,754 - BT3431 0.42 -4.7 4,421,962 + BT3431 0.70 -4.0 4,422,141 + -4.4 4,422,141 + -0.3 4,422,157 - -1.6 4,422,157 - -2.3 4,422,186 + -2.5 4,422,226 - -0.6 4,422,240 + -2.2 4,422,376 + BT3432 0.11 -0.9 4,422,377 - BT3432 0.11 +1.0 4,422,383 - BT3432 0.11 -0.5 4,422,467 + BT3432 0.16 -0.5 4,422,469 + BT3432 0.16 -0.3 4,422,469 + BT3432 0.16 -0.0 4,422,473 - BT3432 0.17 +1.0 4,422,515 - BT3432 0.19 -1.2 4,422,604 + BT3432 0.24 -0.0 4,422,635 + BT3432 0.26 -0.0 4,422,636 - BT3432 0.26 -0.6 4,422,649 + BT3432 0.27 +0.4 4,422,651 + BT3432 0.27 -0.3 4,422,671 - BT3432 0.28 -0.8 4,422,685 + BT3432 0.29 -1.0 4,422,779 - BT3432 0.34 +1.0 4,422,852 - BT3432 0.38 -1.3 4,422,876 + BT3432 0.40 -1.0 4,422,876 + BT3432 0.40 -0.5 4,422,966 - BT3432 0.45 -3.0 4,422,995 - BT3432 0.47 -1.2 4,423,010 - BT3432 0.48 -1.2 4,423,030 + BT3432 0.49 +0.4 4,423,030 + BT3432 0.49 -1.0 4,423,031 - BT3432 0.49 -0.6 4,423,069 + BT3432 0.51 -1.2 4,423,099 - BT3432 0.53 -1.3 4,423,101 + BT3432 0.53 -1.0 4,423,102 - BT3432 0.53 -2.1 4,423,113 - BT3432 0.53 -1.0 4,423,161 + BT3432 0.56 -0.8
Or see this region's nucleotide sequence