Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3337 and ybjU are separated by 233 nucleotides ybjU and SO3339 are separated by 285 nucleotides SO3339 and SO3340 are separated by 31 nucleotides
SO3337: SO3337 - conserved domain protein (NCBI ptt file), at 3,484,043 to 3,485,443
SO3337
SO3338: ybjU - L-allo-threonine aldolase (NCBI ptt file), at 3,485,677 to 3,486,690
ybjU
SO3339: SO3339 - OmpA family protein (NCBI ptt file), at 3,486,976 to 3,487,566
SO3339
SO3340: SO3340 - hypothetical Small-conductance mechanosensitive channel (NCBI ptt file), at 3,487,598 to 3,488,425
SO3340
Position (kb)
3485
3486
3487 Strain fitness (log2 ratio)
-2
-1
0
1 at 3484.680 kb on - strand, within SO3337 at 3484.680 kb on - strand, within SO3337 at 3484.685 kb on - strand, within SO3337 at 3484.780 kb on + strand, within SO3337 at 3484.804 kb on - strand, within SO3337 at 3484.826 kb on + strand, within SO3337 at 3484.838 kb on - strand, within SO3337 at 3484.906 kb on - strand, within SO3337 at 3484.947 kb on - strand, within SO3337 at 3484.957 kb on - strand, within SO3337 at 3485.096 kb on + strand, within SO3337 at 3485.202 kb on + strand, within SO3337 at 3485.207 kb on + strand, within SO3337 at 3485.246 kb on + strand, within SO3337 at 3485.262 kb on - strand, within SO3337 at 3485.310 kb on - strand at 3485.328 kb on - strand at 3485.328 kb on - strand at 3485.359 kb on + strand at 3485.359 kb on + strand at 3485.374 kb on - strand at 3485.405 kb on + strand at 3485.432 kb on - strand at 3485.532 kb on - strand at 3485.666 kb on - strand at 3485.666 kb on - strand at 3485.706 kb on + strand at 3485.760 kb on + strand at 3485.761 kb on + strand at 3485.764 kb on - strand at 3485.766 kb on + strand at 3485.766 kb on + strand at 3485.774 kb on - strand at 3485.774 kb on - strand at 3485.774 kb on - strand at 3485.774 kb on - strand at 3485.779 kb on - strand, within ybjU at 3485.779 kb on - strand, within ybjU at 3485.798 kb on - strand, within ybjU at 3485.807 kb on + strand, within ybjU at 3485.812 kb on + strand, within ybjU at 3485.815 kb on - strand, within ybjU at 3485.820 kb on - strand, within ybjU at 3485.848 kb on + strand, within ybjU at 3485.857 kb on + strand, within ybjU at 3485.857 kb on + strand, within ybjU at 3486.072 kb on - strand, within ybjU at 3486.157 kb on + strand, within ybjU at 3486.157 kb on + strand, within ybjU at 3486.158 kb on - strand, within ybjU at 3486.165 kb on - strand, within ybjU at 3486.183 kb on + strand, within ybjU at 3486.183 kb on + strand, within ybjU at 3486.183 kb on + strand, within ybjU at 3486.183 kb on + strand, within ybjU at 3486.183 kb on + strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.191 kb on - strand, within ybjU at 3486.224 kb on + strand, within ybjU at 3486.232 kb on - strand, within ybjU at 3486.362 kb on - strand, within ybjU at 3486.411 kb on - strand, within ybjU at 3486.465 kb on + strand, within ybjU at 3486.563 kb on + strand, within ybjU at 3486.571 kb on - strand, within ybjU at 3486.607 kb on - strand at 3486.631 kb on - strand at 3486.651 kb on + strand at 3486.746 kb on + strand at 3486.746 kb on + strand at 3486.746 kb on + strand at 3486.751 kb on + strand at 3486.754 kb on - strand at 3486.754 kb on - strand at 3486.755 kb on - strand at 3486.911 kb on + strand at 3486.911 kb on + strand at 3486.911 kb on + strand at 3486.921 kb on + strand at 3486.952 kb on + strand at 3487.039 kb on - strand, within SO3339 at 3487.045 kb on + strand, within SO3339 at 3487.053 kb on - strand, within SO3339 at 3487.066 kb on + strand, within SO3339 at 3487.066 kb on + strand, within SO3339 at 3487.066 kb on + strand, within SO3339 at 3487.068 kb on - strand, within SO3339 at 3487.074 kb on - strand, within SO3339 at 3487.074 kb on - strand, within SO3339 at 3487.074 kb on - strand, within SO3339 at 3487.145 kb on - strand, within SO3339 at 3487.171 kb on - strand, within SO3339 at 3487.194 kb on + strand, within SO3339 at 3487.202 kb on - strand, within SO3339 at 3487.284 kb on + strand, within SO3339 at 3487.291 kb on + strand, within SO3339 at 3487.291 kb on + strand, within SO3339 at 3487.291 kb on + strand, within SO3339 at 3487.291 kb on + strand, within SO3339 at 3487.291 kb on + strand, within SO3339 at 3487.291 kb on + strand, within SO3339 at 3487.291 kb on + strand, within SO3339 at 3487.299 kb on - strand, within SO3339 at 3487.299 kb on - strand, within SO3339 at 3487.299 kb on - strand, within SO3339 at 3487.305 kb on - strand, within SO3339 at 3487.328 kb on - strand, within SO3339 at 3487.336 kb on + strand, within SO3339 at 3487.336 kb on + strand, within SO3339 at 3487.336 kb on + strand, within SO3339 at 3487.336 kb on + strand, within SO3339 at 3487.336 kb on + strand, within SO3339 at 3487.344 kb on - strand, within SO3339 at 3487.344 kb on - strand, within SO3339 at 3487.344 kb on - strand, within SO3339 at 3487.399 kb on + strand, within SO3339 at 3487.460 kb on + strand, within SO3339 at 3487.479 kb on + strand, within SO3339 at 3487.479 kb on + strand, within SO3339 at 3487.479 kb on + strand, within SO3339 at 3487.480 kb on + strand, within SO3339 at 3487.480 kb on + strand, within SO3339 at 3487.487 kb on - strand, within SO3339 at 3487.487 kb on - strand, within SO3339 at 3487.487 kb on - strand, within SO3339 at 3487.487 kb on - strand, within SO3339 at 3487.487 kb on - strand, within SO3339 at 3487.493 kb on - strand, within SO3339 at 3487.498 kb on + strand, within SO3339 at 3487.513 kb on + strand at 3487.625 kb on - strand at 3487.629 kb on + strand at 3487.629 kb on + strand at 3487.637 kb on - strand at 3487.653 kb on - strand at 3487.655 kb on - strand at 3487.660 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 3,484,680 - SO3337 0.45 -0.1 3,484,680 - SO3337 0.45 +0.3 3,484,685 - SO3337 0.46 -0.0 3,484,780 + SO3337 0.53 -1.1 3,484,804 - SO3337 0.54 -0.0 3,484,826 + SO3337 0.56 +0.4 3,484,838 - SO3337 0.57 -0.7 3,484,906 - SO3337 0.62 -0.3 3,484,947 - SO3337 0.65 +0.3 3,484,957 - SO3337 0.65 +1.3 3,485,096 + SO3337 0.75 +0.1 3,485,202 + SO3337 0.83 -0.1 3,485,207 + SO3337 0.83 -0.1 3,485,246 + SO3337 0.86 +0.0 3,485,262 - SO3337 0.87 -2.0 3,485,310 - -1.0 3,485,328 - +0.7 3,485,328 - +0.6 3,485,359 + -0.6 3,485,359 + -0.0 3,485,374 - -0.2 3,485,405 + -0.6 3,485,432 - +0.1 3,485,532 - +0.1 3,485,666 - -0.8 3,485,666 - +0.4 3,485,706 + +0.1 3,485,760 + -0.5 3,485,761 + -0.1 3,485,764 - +0.7 3,485,766 + -0.1 3,485,766 + -0.2 3,485,774 - -0.4 3,485,774 - -0.0 3,485,774 - -1.3 3,485,774 - -0.2 3,485,779 - ybjU SO3338 0.10 -0.3 3,485,779 - ybjU SO3338 0.10 -0.7 3,485,798 - ybjU SO3338 0.12 -0.4 3,485,807 + ybjU SO3338 0.13 -0.1 3,485,812 + ybjU SO3338 0.13 +1.0 3,485,815 - ybjU SO3338 0.14 -0.1 3,485,820 - ybjU SO3338 0.14 -0.5 3,485,848 + ybjU SO3338 0.17 +0.2 3,485,857 + ybjU SO3338 0.18 -0.3 3,485,857 + ybjU SO3338 0.18 -0.8 3,486,072 - ybjU SO3338 0.39 -1.3 3,486,157 + ybjU SO3338 0.47 +0.2 3,486,157 + ybjU SO3338 0.47 -0.9 3,486,158 - ybjU SO3338 0.47 -0.1 3,486,165 - ybjU SO3338 0.48 +0.5 3,486,183 + ybjU SO3338 0.50 -0.8 3,486,183 + ybjU SO3338 0.50 +0.2 3,486,183 + ybjU SO3338 0.50 +0.3 3,486,183 + ybjU SO3338 0.50 +0.1 3,486,183 + ybjU SO3338 0.50 -0.1 3,486,191 - ybjU SO3338 0.51 -0.1 3,486,191 - ybjU SO3338 0.51 -0.3 3,486,191 - ybjU SO3338 0.51 +0.1 3,486,191 - ybjU SO3338 0.51 -1.0 3,486,191 - ybjU SO3338 0.51 +0.0 3,486,191 - ybjU SO3338 0.51 -0.0 3,486,191 - ybjU SO3338 0.51 -0.2 3,486,191 - ybjU SO3338 0.51 -0.2 3,486,191 - ybjU SO3338 0.51 +1.4 3,486,224 + ybjU SO3338 0.54 +1.2 3,486,232 - ybjU SO3338 0.55 -0.3 3,486,362 - ybjU SO3338 0.68 -0.1 3,486,411 - ybjU SO3338 0.72 +0.5 3,486,465 + ybjU SO3338 0.78 -0.8 3,486,563 + ybjU SO3338 0.87 -0.1 3,486,571 - ybjU SO3338 0.88 -0.1 3,486,607 - -0.3 3,486,631 - +0.4 3,486,651 + +1.1 3,486,746 + +0.5 3,486,746 + +0.3 3,486,746 + +0.2 3,486,751 + -1.1 3,486,754 - -0.1 3,486,754 - +0.3 3,486,755 - -0.4 3,486,911 + -0.3 3,486,911 + +0.0 3,486,911 + +0.1 3,486,921 + +0.4 3,486,952 + -0.2 3,487,039 - SO3339 0.11 -0.8 3,487,045 + SO3339 0.12 +1.3 3,487,053 - SO3339 0.13 +0.1 3,487,066 + SO3339 0.15 -0.2 3,487,066 + SO3339 0.15 +0.0 3,487,066 + SO3339 0.15 -0.1 3,487,068 - SO3339 0.16 -0.2 3,487,074 - SO3339 0.17 +1.1 3,487,074 - SO3339 0.17 +0.5 3,487,074 - SO3339 0.17 -0.1 3,487,145 - SO3339 0.29 -0.3 3,487,171 - SO3339 0.33 -0.5 3,487,194 + SO3339 0.37 -1.6 3,487,202 - SO3339 0.38 +0.1 3,487,284 + SO3339 0.52 -0.5 3,487,291 + SO3339 0.53 +0.5 3,487,291 + SO3339 0.53 -0.8 3,487,291 + SO3339 0.53 +0.2 3,487,291 + SO3339 0.53 +0.2 3,487,291 + SO3339 0.53 -0.1 3,487,291 + SO3339 0.53 -0.4 3,487,291 + SO3339 0.53 +0.2 3,487,299 - SO3339 0.55 +0.7 3,487,299 - SO3339 0.55 -0.1 3,487,299 - SO3339 0.55 +0.4 3,487,305 - SO3339 0.56 +0.0 3,487,328 - SO3339 0.60 -0.5 3,487,336 + SO3339 0.61 -0.2 3,487,336 + SO3339 0.61 -0.3 3,487,336 + SO3339 0.61 +0.1 3,487,336 + SO3339 0.61 -0.5 3,487,336 + SO3339 0.61 +0.0 3,487,344 - SO3339 0.62 -0.1 3,487,344 - SO3339 0.62 +0.4 3,487,344 - SO3339 0.62 -0.6 3,487,399 + SO3339 0.72 +0.1 3,487,460 + SO3339 0.82 +0.3 3,487,479 + SO3339 0.85 -1.1 3,487,479 + SO3339 0.85 -0.4 3,487,479 + SO3339 0.85 -0.3 3,487,480 + SO3339 0.85 -0.2 3,487,480 + SO3339 0.85 +0.5 3,487,487 - SO3339 0.86 +0.2 3,487,487 - SO3339 0.86 +0.3 3,487,487 - SO3339 0.86 -0.0 3,487,487 - SO3339 0.86 -0.6 3,487,487 - SO3339 0.86 +0.1 3,487,493 - SO3339 0.87 +0.5 3,487,498 + SO3339 0.88 -0.7 3,487,513 + +0.5 3,487,625 - +0.3 3,487,629 + -0.4 3,487,629 + -0.3 3,487,637 - +0.8 3,487,653 - +0.5 3,487,655 - +0.2 3,487,660 + +1.0
Or see this region's nucleotide sequence