Strain Fitness in Shewanella oneidensis MR-1 around SO2431

Experiment: Cholin acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntruvB and ruvA are separated by 85 nucleotidesruvA and ruvC overlap by 4 nucleotidesruvC and SO2432 are separated by 188 nucleotides SO2429: ruvB - Holliday junction DNA helicase RuvB (NCBI ptt file), at 2,538,186 to 2,539,190 ruvB SO2430: ruvA - Holliday junction DNA helicase RuvA (NCBI ptt file), at 2,539,276 to 2,539,893 ruvA SO2431: ruvC - crossover junction endodeoxyribonuclease RuvC (NCBI ptt file), at 2,539,890 to 2,540,411 ruvC SO2432: SO2432 - conserved hypothetical protein TIGR01033 (NCBI ptt file), at 2,540,600 to 2,541,346 SO2432 Position (kb) 2539 2540 2541Strain fitness (log2 ratio) -2 -1 0 1at 2539.094 kb on - strandat 2539.131 kb on + strandat 2539.233 kb on - strandat 2539.326 kb on - strandat 2539.326 kb on - strandat 2539.405 kb on + strand, within ruvAat 2539.481 kb on - strand, within ruvAat 2539.503 kb on - strand, within ruvAat 2539.584 kb on + strand, within ruvAat 2539.886 kb on + strandat 2539.891 kb on + strandat 2539.964 kb on - strand, within ruvCat 2540.395 kb on - strandat 2540.475 kb on + strandat 2540.483 kb on - strandat 2540.570 kb on - strandat 2540.855 kb on - strand, within SO2432at 2540.924 kb on + strand, within SO2432at 2540.980 kb on - strand, within SO2432at 2541.074 kb on + strand, within SO2432at 2541.311 kb on - strandat 2541.356 kb on + strandat 2541.362 kb on - strandat 2541.364 kb on - strandat 2541.364 kb on - strandat 2541.364 kb on - strandat 2541.390 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 20 mM
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2,539,094 - -0.6
2,539,131 + -0.5
2,539,233 - -1.1
2,539,326 - -1.6
2,539,326 - -0.9
2,539,405 + ruvA SO2430 0.21 -1.5
2,539,481 - ruvA SO2430 0.33 -0.9
2,539,503 - ruvA SO2430 0.37 -0.2
2,539,584 + ruvA SO2430 0.50 -1.6
2,539,886 + -1.8
2,539,891 + -1.3
2,539,964 - ruvC SO2431 0.14 -1.3
2,540,395 - -0.6
2,540,475 + -0.3
2,540,483 - +0.1
2,540,570 - -0.2
2,540,855 - SO2432 0.34 +0.0
2,540,924 + SO2432 0.43 -1.0
2,540,980 - SO2432 0.51 +0.0
2,541,074 + SO2432 0.63 +0.2
2,541,311 - -0.5
2,541,356 + +0.1
2,541,362 - -0.4
2,541,364 - +0.3
2,541,364 - -0.0
2,541,364 - -0.1
2,541,390 + -0.3

Or see this region's nucleotide sequence