Strain Fitness in Shewanella oneidensis MR-1 around SO1347

Experiment: Cholin acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlepA and lepB are separated by 178 nucleotideslepB and rnc are separated by 1 nucleotidesrnc and era overlap by 4 nucleotides SO1346: lepA - GTP-binding protein LepA (NCBI ptt file), at 1,402,038 to 1,403,828 lepA SO1347: lepB - signal peptidase I (NCBI ptt file), at 1,404,007 to 1,404,924 lepB SO1348: rnc - ribonuclease III (NCBI ptt file), at 1,404,926 to 1,405,606 rnc SO1349: era - GTP-binding protein Era (NCBI ptt file), at 1,405,603 to 1,406,622 era Position (kb) 1404 1405Strain fitness (log2 ratio) -2 -1 0 1at 1403.034 kb on + strand, within lepAat 1403.034 kb on + strand, within lepAat 1403.067 kb on + strand, within lepAat 1403.628 kb on + strand, within lepAat 1403.693 kb on + strandat 1403.693 kb on + strandat 1403.878 kb on + strandat 1404.156 kb on + strand, within lepBat 1404.445 kb on + strand, within lepBat 1404.467 kb on + strand, within lepBat 1404.559 kb on + strand, within lepBat 1404.608 kb on + strand, within lepBat 1404.764 kb on + strand, within lepBat 1404.875 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 20 mM
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1,403,034 + lepA SO1346 0.56 -0.4
1,403,034 + lepA SO1346 0.56 -1.2
1,403,067 + lepA SO1346 0.57 -0.0
1,403,628 + lepA SO1346 0.89 -1.0
1,403,693 + +0.4
1,403,693 + -0.8
1,403,878 + -0.9
1,404,156 + lepB SO1347 0.16 -1.8
1,404,445 + lepB SO1347 0.48 -1.4
1,404,467 + lepB SO1347 0.50 +0.1
1,404,559 + lepB SO1347 0.60 -0.7
1,404,608 + lepB SO1347 0.65 -0.2
1,404,764 + lepB SO1347 0.82 -0.7
1,404,875 + -1.3

Or see this region's nucleotide sequence