Experiment: Choline chloride 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pntB and SO3741.1 are separated by 171 nucleotides SO3741.1 and SO3743 are separated by 78 nucleotides SO3743 and SO3744 are separated by 153 nucleotides
SO3741: pntB - NAD(P) transhydrogenase, beta subunit (NCBI ptt file), at 3,886,512 to 3,887,921
pntB
SO3741.1: SO3741.1 - hypothetical protein (NCBI ptt file), at 3,888,093 to 3,888,482
SO3741.1
SO3743: SO3743 - transcriptional regulator, TetR family (NCBI ptt file), at 3,888,561 to 3,889,040
SO3743
SO3744: SO3744 - hypothetical protein (NCBI ptt file), at 3,889,194 to 3,890,021
SO3744
Position (kb)
3888
3889 Strain fitness (log2 ratio)
-1
0
1
2 at 3887.116 kb on + strand, within pntB at 3887.297 kb on + strand, within pntB at 3887.309 kb on + strand, within pntB at 3887.529 kb on - strand, within pntB at 3887.542 kb on + strand, within pntB at 3887.579 kb on - strand, within pntB at 3887.627 kb on - strand, within pntB at 3887.682 kb on - strand, within pntB at 3887.765 kb on + strand, within pntB at 3887.813 kb on + strand at 3887.891 kb on - strand at 3887.893 kb on + strand at 3888.017 kb on + strand at 3888.152 kb on + strand, within SO3741.1 at 3888.183 kb on - strand, within SO3741.1 at 3888.183 kb on - strand, within SO3741.1 at 3888.286 kb on + strand, within SO3741.1 at 3888.286 kb on + strand, within SO3741.1 at 3888.386 kb on + strand, within SO3741.1 at 3888.404 kb on - strand, within SO3741.1 at 3888.418 kb on + strand, within SO3741.1 at 3888.484 kb on - strand at 3888.498 kb on + strand at 3888.682 kb on - strand, within SO3743 at 3888.682 kb on - strand, within SO3743 at 3888.696 kb on - strand, within SO3743 at 3888.696 kb on - strand, within SO3743 at 3888.696 kb on - strand, within SO3743 at 3888.696 kb on - strand, within SO3743 at 3888.749 kb on - strand, within SO3743 at 3888.754 kb on - strand, within SO3743 at 3888.756 kb on + strand, within SO3743 at 3888.756 kb on + strand, within SO3743 at 3888.756 kb on + strand, within SO3743 at 3888.764 kb on - strand, within SO3743 at 3888.901 kb on - strand, within SO3743 at 3888.945 kb on - strand, within SO3743 at 3888.945 kb on - strand, within SO3743 at 3889.018 kb on - strand at 3889.216 kb on + strand at 3889.216 kb on + strand at 3889.248 kb on + strand at 3889.346 kb on + strand, within SO3744
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 150 mM remove 3,887,116 + pntB SO3741 0.43 -0.1 3,887,297 + pntB SO3741 0.56 -0.5 3,887,309 + pntB SO3741 0.57 -0.3 3,887,529 - pntB SO3741 0.72 +0.1 3,887,542 + pntB SO3741 0.73 +0.7 3,887,579 - pntB SO3741 0.76 +1.2 3,887,627 - pntB SO3741 0.79 -0.3 3,887,682 - pntB SO3741 0.83 +2.6 3,887,765 + pntB SO3741 0.89 +0.9 3,887,813 + -0.1 3,887,891 - +0.4 3,887,893 + -0.9 3,888,017 + -0.2 3,888,152 + SO3741.1 0.15 -0.4 3,888,183 - SO3741.1 0.23 -0.0 3,888,183 - SO3741.1 0.23 -1.0 3,888,286 + SO3741.1 0.49 -1.4 3,888,286 + SO3741.1 0.49 -0.8 3,888,386 + SO3741.1 0.75 +0.1 3,888,404 - SO3741.1 0.80 -0.5 3,888,418 + SO3741.1 0.83 -0.2 3,888,484 - -0.2 3,888,498 + -0.6 3,888,682 - SO3743 0.25 -0.0 3,888,682 - SO3743 0.25 -1.4 3,888,696 - SO3743 0.28 -1.3 3,888,696 - SO3743 0.28 +0.7 3,888,696 - SO3743 0.28 -1.7 3,888,696 - SO3743 0.28 -0.3 3,888,749 - SO3743 0.39 +0.8 3,888,754 - SO3743 0.40 -1.0 3,888,756 + SO3743 0.41 +0.6 3,888,756 + SO3743 0.41 +0.5 3,888,756 + SO3743 0.41 +0.9 3,888,764 - SO3743 0.42 +1.0 3,888,901 - SO3743 0.71 +0.3 3,888,945 - SO3743 0.80 -0.3 3,888,945 - SO3743 0.80 +0.1 3,889,018 - +0.6 3,889,216 + +0.1 3,889,216 + +0.3 3,889,248 + +1.7 3,889,346 + SO3744 0.18 -0.4
Or see this region's nucleotide sequence