Strain Fitness in Shewanella oneidensis MR-1 around SO3433

Experiment: Choline chloride 150 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpoS and nlpD are separated by 81 nucleotidesnlpD and pcm are separated by 57 nucleotidespcm and surE overlap by 4 nucleotides SO3432: rpoS - RNA polymerase sigma-38 factor (NCBI ptt file), at 3,577,258 to 3,578,238 rpoS SO3433: nlpD - lipoprotein NlpD (NCBI ptt file), at 3,578,320 to 3,579,216 nlpD SO3434: pcm - protein-L-isoaspartate O-methyltransferase (NCBI ptt file), at 3,579,274 to 3,579,909 pcm SO3435: surE - stationary-phase survival protein SurE (NCBI ptt file), at 3,579,906 to 3,580,652 surE Position (kb) 3578 3579 3580Strain fitness (log2 ratio) -2 -1 0 1 2at 3577.344 kb on - strandat 3577.448 kb on + strand, within rpoSat 3577.456 kb on - strand, within rpoSat 3577.495 kb on + strand, within rpoSat 3577.528 kb on - strand, within rpoSat 3577.552 kb on + strand, within rpoSat 3577.626 kb on + strand, within rpoSat 3577.634 kb on - strand, within rpoSat 3577.634 kb on - strand, within rpoSat 3577.639 kb on + strand, within rpoSat 3577.640 kb on - strand, within rpoSat 3577.721 kb on + strand, within rpoSat 3577.729 kb on - strand, within rpoSat 3577.765 kb on + strand, within rpoSat 3577.956 kb on + strand, within rpoSat 3578.074 kb on - strand, within rpoSat 3578.168 kb on + strandat 3578.338 kb on + strandat 3578.343 kb on - strandat 3578.353 kb on - strandat 3578.394 kb on + strandat 3578.402 kb on - strandat 3578.464 kb on + strand, within nlpDat 3578.464 kb on + strand, within nlpDat 3578.642 kb on - strand, within nlpDat 3578.685 kb on + strand, within nlpDat 3578.685 kb on + strand, within nlpDat 3578.685 kb on + strand, within nlpDat 3578.693 kb on - strand, within nlpDat 3578.693 kb on - strand, within nlpDat 3578.693 kb on - strand, within nlpDat 3578.711 kb on - strand, within nlpDat 3578.958 kb on + strand, within nlpDat 3578.966 kb on - strand, within nlpDat 3579.092 kb on + strand, within nlpDat 3579.163 kb on - strandat 3579.185 kb on - strandat 3579.266 kb on + strandat 3579.316 kb on + strandat 3579.322 kb on - strandat 3579.324 kb on - strandat 3579.546 kb on + strand, within pcmat 3579.587 kb on - strand, within pcmat 3579.640 kb on + strand, within pcmat 3579.655 kb on - strand, within pcmat 3579.721 kb on - strand, within pcmat 3579.736 kb on + strand, within pcmat 3579.744 kb on - strand, within pcmat 3579.750 kb on - strand, within pcmat 3580.043 kb on + strand, within surEat 3580.141 kb on + strand, within surE

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline chloride 150 mM
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3,577,344 - -0.1
3,577,448 + rpoS SO3432 0.19 +0.5
3,577,456 - rpoS SO3432 0.20 +0.2
3,577,495 + rpoS SO3432 0.24 +0.0
3,577,528 - rpoS SO3432 0.28 +0.2
3,577,552 + rpoS SO3432 0.30 +0.3
3,577,626 + rpoS SO3432 0.38 -0.6
3,577,634 - rpoS SO3432 0.38 -0.0
3,577,634 - rpoS SO3432 0.38 -0.4
3,577,639 + rpoS SO3432 0.39 -0.9
3,577,640 - rpoS SO3432 0.39 -0.7
3,577,721 + rpoS SO3432 0.47 +0.2
3,577,729 - rpoS SO3432 0.48 -0.2
3,577,765 + rpoS SO3432 0.52 -0.5
3,577,956 + rpoS SO3432 0.71 +2.5
3,578,074 - rpoS SO3432 0.83 +0.1
3,578,168 + +0.1
3,578,338 + -0.9
3,578,343 - +0.4
3,578,353 - -0.7
3,578,394 + -0.2
3,578,402 - +0.4
3,578,464 + nlpD SO3433 0.16 +0.5
3,578,464 + nlpD SO3433 0.16 -0.8
3,578,642 - nlpD SO3433 0.36 -0.3
3,578,685 + nlpD SO3433 0.41 -1.2
3,578,685 + nlpD SO3433 0.41 -0.6
3,578,685 + nlpD SO3433 0.41 -2.4
3,578,693 - nlpD SO3433 0.42 -1.5
3,578,693 - nlpD SO3433 0.42 -0.8
3,578,693 - nlpD SO3433 0.42 -1.5
3,578,711 - nlpD SO3433 0.44 -1.6
3,578,958 + nlpD SO3433 0.71 -0.4
3,578,966 - nlpD SO3433 0.72 +0.6
3,579,092 + nlpD SO3433 0.86 -0.9
3,579,163 - +1.5
3,579,185 - -1.5
3,579,266 + -0.5
3,579,316 + +0.2
3,579,322 - -0.4
3,579,324 - +0.2
3,579,546 + pcm SO3434 0.43 -0.7
3,579,587 - pcm SO3434 0.49 -1.3
3,579,640 + pcm SO3434 0.58 -1.0
3,579,655 - pcm SO3434 0.60 -1.6
3,579,721 - pcm SO3434 0.70 +1.0
3,579,736 + pcm SO3434 0.73 -0.3
3,579,744 - pcm SO3434 0.74 -0.4
3,579,750 - pcm SO3434 0.75 +1.8
3,580,043 + surE SO3435 0.18 -2.1
3,580,141 + surE SO3435 0.31 -0.5

Or see this region's nucleotide sequence