Experiment: Choline chloride 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purF and SO3065 are separated by 182 nucleotides SO3065 and SO3066 are separated by 109 nucleotides SO3066 and folC overlap by 8 nucleotides
SO3064: purF - amidophosphoribosyltransferase (NCBI ptt file), at 3,180,212 to 3,181,726
purF
SO3065: SO3065 - colicin V production protein (NCBI ptt file), at 3,181,909 to 3,182,397
SO3065
SO3066: SO3066 - conserved hypothetical protein (NCBI ptt file), at 3,182,507 to 3,183,352
SO3066
SO3067: folC - FolC bifunctional protein (NCBI ptt file), at 3,183,345 to 3,184,640
folC
Position (kb)
3182
3183
3184 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3181.554 kb on - strand, within purF at 3181.587 kb on + strand at 3181.595 kb on - strand at 3181.645 kb on - strand at 3181.652 kb on - strand at 3182.017 kb on + strand, within SO3065 at 3182.025 kb on - strand, within SO3065 at 3182.059 kb on + strand, within SO3065 at 3182.420 kb on + strand at 3182.428 kb on - strand at 3182.448 kb on - strand at 3182.590 kb on - strand at 3182.638 kb on + strand, within SO3066 at 3182.658 kb on + strand, within SO3066 at 3182.658 kb on + strand, within SO3066 at 3182.658 kb on + strand, within SO3066 at 3182.666 kb on - strand, within SO3066 at 3182.837 kb on - strand, within SO3066 at 3182.975 kb on - strand, within SO3066 at 3183.153 kb on - strand, within SO3066
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 150 mM remove 3,181,554 - purF SO3064 0.89 -1.3 3,181,587 + +0.4 3,181,595 - -0.5 3,181,645 - -0.0 3,181,652 - -0.2 3,182,017 + SO3065 0.22 -1.7 3,182,025 - SO3065 0.24 -1.4 3,182,059 + SO3065 0.31 +1.4 3,182,420 + -1.0 3,182,428 - +1.2 3,182,448 - -0.5 3,182,590 - -2.8 3,182,638 + SO3066 0.15 -1.3 3,182,658 + SO3066 0.18 -1.3 3,182,658 + SO3066 0.18 -3.3 3,182,658 + SO3066 0.18 -1.0 3,182,666 - SO3066 0.19 -2.3 3,182,837 - SO3066 0.39 -1.4 3,182,975 - SO3066 0.55 -0.7 3,183,153 - SO3066 0.76 -0.2
Or see this region's nucleotide sequence