Experiment: Choline chloride 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1319 and SO1320 overlap by 4 nucleotides SO1320 and SO1321 are separated by 43 nucleotides SO1321 and pfs are separated by 31 nucleotides pfs and SO1323 overlap by 2 nucleotides
SO1319: SO1319 - putative transporter, required for glycine utilization (from data) , at 1,373,879 to 1,374,505
SO1319
SO1320: SO1320 - hypothetical protein (NCBI ptt file), at 1,374,502 to 1,374,891
SO1320
SO1321: SO1321 - CobD-related protein (NCBI ptt file), at 1,374,935 to 1,375,924
SO1321
SO1322: pfs - 5-methylthioadenosine nucleosidase/S-adenosylhomocysteine nucleosidase (NCBI ptt file), at 1,375,956 to 1,376,666
pfs
SO1323: SO1323 - hypothetical protein (NCBI ptt file), at 1,376,665 to 1,376,871
SO1323
Position (kb)
1374
1375
1376 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1373.968 kb on - strand, within SO1319 at 1373.968 kb on - strand, within SO1319 at 1374.010 kb on - strand, within SO1319 at 1374.019 kb on + strand, within SO1319 at 1374.023 kb on - strand, within SO1319 at 1374.023 kb on - strand, within SO1319 at 1374.027 kb on - strand, within SO1319 at 1374.084 kb on + strand, within SO1319 at 1374.202 kb on + strand, within SO1319 at 1374.202 kb on + strand, within SO1319 at 1374.202 kb on + strand, within SO1319 at 1374.205 kb on - strand, within SO1319 at 1374.286 kb on - strand, within SO1319 at 1374.376 kb on - strand, within SO1319 at 1374.381 kb on + strand, within SO1319 at 1374.487 kb on + strand at 1374.658 kb on + strand, within SO1320 at 1374.669 kb on + strand, within SO1320 at 1374.669 kb on + strand, within SO1320 at 1374.669 kb on + strand, within SO1320 at 1374.677 kb on - strand, within SO1320 at 1374.781 kb on + strand, within SO1320 at 1374.942 kb on + strand at 1374.953 kb on + strand at 1374.953 kb on + strand at 1374.953 kb on + strand at 1374.953 kb on + strand at 1374.961 kb on - strand at 1374.961 kb on - strand at 1374.961 kb on - strand at 1375.076 kb on - strand, within SO1321 at 1375.227 kb on - strand, within SO1321 at 1375.284 kb on + strand, within SO1321 at 1375.477 kb on - strand, within SO1321 at 1375.509 kb on + strand, within SO1321 at 1375.509 kb on + strand, within SO1321 at 1375.509 kb on + strand, within SO1321 at 1375.509 kb on + strand, within SO1321 at 1375.545 kb on + strand, within SO1321 at 1375.546 kb on - strand, within SO1321 at 1375.743 kb on + strand, within SO1321 at 1375.931 kb on - strand at 1376.761 kb on + strand, within SO1323 at 1376.816 kb on - strand, within SO1323 at 1376.823 kb on + strand, within SO1323 at 1376.823 kb on + strand, within SO1323 at 1376.830 kb on - strand, within SO1323 at 1376.873 kb on - strand at 1376.895 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 150 mM remove 1,373,968 - SO1319 0.14 +0.4 1,373,968 - SO1319 0.14 -2.9 1,374,010 - SO1319 0.21 +0.6 1,374,019 + SO1319 0.22 +0.0 1,374,023 - SO1319 0.23 +0.9 1,374,023 - SO1319 0.23 -0.4 1,374,027 - SO1319 0.24 -0.6 1,374,084 + SO1319 0.33 +0.5 1,374,202 + SO1319 0.52 -0.6 1,374,202 + SO1319 0.52 -0.2 1,374,202 + SO1319 0.52 +0.3 1,374,205 - SO1319 0.52 +0.5 1,374,286 - SO1319 0.65 +0.2 1,374,376 - SO1319 0.79 +0.1 1,374,381 + SO1319 0.80 +0.0 1,374,487 + +0.5 1,374,658 + SO1320 0.40 -1.2 1,374,669 + SO1320 0.43 -0.2 1,374,669 + SO1320 0.43 +0.5 1,374,669 + SO1320 0.43 +0.0 1,374,677 - SO1320 0.45 +0.2 1,374,781 + SO1320 0.72 -0.9 1,374,942 + -0.4 1,374,953 + +0.5 1,374,953 + -0.3 1,374,953 + +0.2 1,374,953 + +0.1 1,374,961 - -0.0 1,374,961 - -0.7 1,374,961 - -0.0 1,375,076 - SO1321 0.14 +0.4 1,375,227 - SO1321 0.29 +0.0 1,375,284 + SO1321 0.35 +0.6 1,375,477 - SO1321 0.55 -0.1 1,375,509 + SO1321 0.58 -1.0 1,375,509 + SO1321 0.58 +0.3 1,375,509 + SO1321 0.58 +0.0 1,375,509 + SO1321 0.58 +0.0 1,375,545 + SO1321 0.62 +0.8 1,375,546 - SO1321 0.62 -0.4 1,375,743 + SO1321 0.82 +0.6 1,375,931 - +0.3 1,376,761 + SO1323 0.46 -0.1 1,376,816 - SO1323 0.73 -0.8 1,376,823 + SO1323 0.76 -0.0 1,376,823 + SO1323 0.76 -0.4 1,376,830 - SO1323 0.80 -0.2 1,376,873 - -0.3 1,376,895 + -0.2
Or see this region's nucleotide sequence