Experiment: Choline chloride 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0190 and cysQ-1 are separated by 69 nucleotides cysQ-1 and SO0192 are separated by 7 nucleotides SO0192 and SO0193 are separated by 235 nucleotides SO0193 and SO0194 overlap by 10 nucleotides
SO0190: SO0190 - MutT/nudix family protein (NCBI ptt file), at 200,294 to 200,845
SO0190
SO0191: cysQ-1 - cysQ protein (NCBI ptt file), at 200,915 to 201,727
cysQ-1
SO0192: SO0192 - acetyltransferase, GNAT family (NCBI ptt file), at 201,735 to 202,178
SO0192
SO0193: SO0193 - transcriptional regulator, TetR family (NCBI ptt file), at 202,414 to 203,025
SO0193
SO0194: SO0194 - acyltransferase family protein (NCBI ptt file), at 203,016 to 204,878
SO0194
Position (kb) 201
202
203 Strain fitness (log2 ratio) -2
-1
0
1
2 at 200.737 kb on + strand, within SO0190 at 200.743 kb on + strand, within SO0190 at 200.751 kb on - strand, within SO0190 at 200.965 kb on + strand at 200.989 kb on + strand at 201.040 kb on - strand, within cysQ-1 at 201.194 kb on + strand, within cysQ-1 at 201.241 kb on + strand, within cysQ-1 at 201.292 kb on + strand, within cysQ-1 at 201.371 kb on + strand, within cysQ-1 at 201.379 kb on - strand, within cysQ-1 at 201.392 kb on + strand, within cysQ-1 at 201.399 kb on + strand, within cysQ-1 at 201.400 kb on - strand, within cysQ-1 at 201.454 kb on + strand, within cysQ-1 at 201.499 kb on - strand, within cysQ-1 at 201.499 kb on - strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.512 kb on + strand, within cysQ-1 at 201.629 kb on + strand, within cysQ-1 at 201.696 kb on - strand at 201.789 kb on + strand, within SO0192 at 201.820 kb on - strand, within SO0192 at 201.915 kb on + strand, within SO0192 at 201.966 kb on + strand, within SO0192 at 201.979 kb on + strand, within SO0192 at 201.979 kb on + strand, within SO0192 at 201.979 kb on + strand, within SO0192 at 201.987 kb on - strand, within SO0192 at 201.992 kb on - strand, within SO0192 at 201.998 kb on - strand, within SO0192 at 202.012 kb on + strand, within SO0192 at 202.020 kb on - strand, within SO0192 at 202.034 kb on + strand, within SO0192 at 202.034 kb on + strand, within SO0192 at 202.034 kb on + strand, within SO0192 at 202.034 kb on + strand, within SO0192 at 202.042 kb on - strand, within SO0192 at 202.042 kb on - strand, within SO0192 at 202.042 kb on - strand, within SO0192 at 202.115 kb on + strand, within SO0192 at 202.191 kb on - strand at 202.273 kb on + strand at 202.288 kb on - strand at 202.295 kb on - strand at 202.323 kb on - strand at 202.411 kb on - strand at 202.417 kb on - strand at 202.429 kb on + strand at 202.505 kb on + strand, within SO0193 at 202.565 kb on - strand, within SO0193 at 202.617 kb on - strand, within SO0193 at 202.716 kb on - strand, within SO0193 at 202.721 kb on + strand, within SO0193 at 202.721 kb on + strand, within SO0193 at 202.729 kb on - strand, within SO0193 at 202.729 kb on - strand, within SO0193 at 202.744 kb on + strand, within SO0193 at 202.754 kb on + strand, within SO0193 at 202.788 kb on - strand, within SO0193 at 202.828 kb on - strand, within SO0193 at 202.830 kb on + strand, within SO0193 at 202.878 kb on + strand, within SO0193 at 202.878 kb on + strand, within SO0193 at 202.952 kb on - strand, within SO0193 at 203.084 kb on - strand at 203.113 kb on + strand at 203.121 kb on - strand at 203.121 kb on - strand at 203.132 kb on + strand at 203.140 kb on - strand at 203.143 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 150 mM remove 200,737 + SO0190 0.80 -0.5 200,743 + SO0190 0.81 +0.3 200,751 - SO0190 0.83 +1.4 200,965 + +0.9 200,989 + +1.6 201,040 - cysQ-1 SO0191 0.15 +1.1 201,194 + cysQ-1 SO0191 0.34 +0.3 201,241 + cysQ-1 SO0191 0.40 +0.3 201,292 + cysQ-1 SO0191 0.46 +0.5 201,371 + cysQ-1 SO0191 0.56 +1.3 201,379 - cysQ-1 SO0191 0.57 +1.4 201,392 + cysQ-1 SO0191 0.59 +0.9 201,399 + cysQ-1 SO0191 0.60 +1.1 201,400 - cysQ-1 SO0191 0.60 -0.0 201,454 + cysQ-1 SO0191 0.66 +0.4 201,499 - cysQ-1 SO0191 0.72 +0.1 201,499 - cysQ-1 SO0191 0.72 +1.6 201,512 + cysQ-1 SO0191 0.73 +0.2 201,512 + cysQ-1 SO0191 0.73 +0.6 201,512 + cysQ-1 SO0191 0.73 +0.6 201,512 + cysQ-1 SO0191 0.73 +0.4 201,512 + cysQ-1 SO0191 0.73 +0.3 201,629 + cysQ-1 SO0191 0.88 +1.3 201,696 - +1.0 201,789 + SO0192 0.12 -0.5 201,820 - SO0192 0.19 -0.0 201,915 + SO0192 0.41 +0.7 201,966 + SO0192 0.52 +0.0 201,979 + SO0192 0.55 +0.8 201,979 + SO0192 0.55 -0.2 201,979 + SO0192 0.55 +0.4 201,987 - SO0192 0.57 +0.4 201,992 - SO0192 0.58 -0.0 201,998 - SO0192 0.59 +0.1 202,012 + SO0192 0.62 +0.0 202,020 - SO0192 0.64 -0.5 202,034 + SO0192 0.67 -0.4 202,034 + SO0192 0.67 -0.2 202,034 + SO0192 0.67 -0.3 202,034 + SO0192 0.67 +0.7 202,042 - SO0192 0.69 -0.1 202,042 - SO0192 0.69 +0.0 202,042 - SO0192 0.69 +0.0 202,115 + SO0192 0.86 -0.3 202,191 - +0.7 202,273 + +0.3 202,288 - +0.4 202,295 - -0.4 202,323 - -0.1 202,411 - +1.9 202,417 - +0.9 202,429 + +0.5 202,505 + SO0193 0.15 +0.4 202,565 - SO0193 0.25 +0.9 202,617 - SO0193 0.33 +0.4 202,716 - SO0193 0.49 +0.6 202,721 + SO0193 0.50 +0.6 202,721 + SO0193 0.50 +0.1 202,729 - SO0193 0.51 +1.7 202,729 - SO0193 0.51 +0.8 202,744 + SO0193 0.54 -1.8 202,754 + SO0193 0.56 -0.3 202,788 - SO0193 0.61 +1.1 202,828 - SO0193 0.68 +1.0 202,830 + SO0193 0.68 +0.2 202,878 + SO0193 0.76 -0.1 202,878 + SO0193 0.76 +0.0 202,952 - SO0193 0.88 +1.2 203,084 - +0.6 203,113 + +0.9 203,121 - +1.1 203,121 - +1.0 203,132 + +1.5 203,140 - +0.6 203,143 - +0.7
Or see this region's nucleotide sequence