Experiment: Choline chloride 150 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribB and ptrB are separated by 695 nucleotides ptrB and SO0145 are separated by 22 nucleotides SO0145 and SO0146 are separated by 110 nucleotides
SO0142: ribB - 3,4-dihydroxy-2-butanone 4-phosphate synthase (NCBI ptt file), at 150,203 to 150,856
ribB
SO0144: ptrB - protease II (NCBI ptt file), at 151,552 to 153,654
ptrB
SO0145: SO0145 - hypothetical URI domain endonuclease (NCBI ptt file), at 153,677 to 154,045
SO0145
SO0146: SO0146 - ISSod3, transposase (NCBI ptt file), at 154,156 to 155,370
SO0146
Position (kb) 151
152
153
154 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 150.589 kb on + strand, within ribB at 150.712 kb on - strand, within ribB at 150.749 kb on + strand, within ribB at 150.780 kb on + strand, within ribB at 150.827 kb on + strand at 150.840 kb on + strand at 150.939 kb on + strand at 150.942 kb on - strand at 150.942 kb on - strand at 151.013 kb on - strand at 151.084 kb on - strand at 151.098 kb on + strand at 151.106 kb on - strand at 151.141 kb on + strand at 151.192 kb on - strand at 151.312 kb on + strand at 151.432 kb on + strand at 151.518 kb on + strand at 151.536 kb on - strand at 151.546 kb on - strand at 151.590 kb on - strand at 151.707 kb on + strand at 151.707 kb on + strand at 151.707 kb on + strand at 151.707 kb on + strand at 151.707 kb on + strand at 151.707 kb on + strand at 151.715 kb on - strand at 151.715 kb on - strand at 151.715 kb on - strand at 151.715 kb on - strand at 151.715 kb on - strand at 151.715 kb on - strand at 151.715 kb on - strand at 151.734 kb on + strand at 151.747 kb on - strand at 151.757 kb on - strand at 151.812 kb on - strand, within ptrB at 151.841 kb on + strand, within ptrB at 151.896 kb on - strand, within ptrB at 151.925 kb on + strand, within ptrB at 151.925 kb on + strand, within ptrB at 151.937 kb on - strand, within ptrB at 152.025 kb on - strand, within ptrB at 152.100 kb on + strand, within ptrB at 152.108 kb on - strand, within ptrB at 152.136 kb on + strand, within ptrB at 152.203 kb on + strand, within ptrB at 152.211 kb on - strand, within ptrB at 152.218 kb on - strand, within ptrB at 152.231 kb on - strand, within ptrB at 152.231 kb on - strand, within ptrB at 152.248 kb on + strand, within ptrB at 152.270 kb on + strand, within ptrB at 152.278 kb on - strand, within ptrB at 152.347 kb on + strand, within ptrB at 152.348 kb on + strand, within ptrB at 152.348 kb on + strand, within ptrB at 152.356 kb on - strand, within ptrB at 152.358 kb on + strand, within ptrB at 152.358 kb on + strand, within ptrB at 152.366 kb on - strand, within ptrB at 152.402 kb on + strand, within ptrB at 152.549 kb on + strand, within ptrB at 152.583 kb on - strand, within ptrB at 152.612 kb on - strand, within ptrB at 152.675 kb on + strand, within ptrB at 152.728 kb on + strand, within ptrB at 152.728 kb on + strand, within ptrB at 152.768 kb on - strand, within ptrB at 152.801 kb on + strand, within ptrB at 152.870 kb on + strand, within ptrB at 153.022 kb on + strand, within ptrB at 153.150 kb on + strand, within ptrB at 153.185 kb on - strand, within ptrB at 153.194 kb on + strand, within ptrB at 153.232 kb on + strand, within ptrB at 153.279 kb on + strand, within ptrB at 153.298 kb on - strand, within ptrB at 153.393 kb on - strand, within ptrB at 153.393 kb on - strand, within ptrB at 153.393 kb on - strand, within ptrB at 153.436 kb on + strand, within ptrB at 153.436 kb on + strand, within ptrB at 153.436 kb on + strand, within ptrB at 153.446 kb on + strand at 153.602 kb on - strand at 153.626 kb on + strand at 153.657 kb on + strand at 153.701 kb on + strand at 153.747 kb on + strand, within SO0145 at 153.747 kb on + strand, within SO0145 at 153.748 kb on - strand, within SO0145 at 153.803 kb on - strand, within SO0145 at 153.880 kb on - strand, within SO0145 at 153.965 kb on + strand, within SO0145 at 153.973 kb on - strand, within SO0145 at 154.039 kb on - strand at 154.096 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 150 mM remove 150,589 + ribB SO0142 0.59 +1.3 150,712 - ribB SO0142 0.78 -0.1 150,749 + ribB SO0142 0.83 -0.1 150,780 + ribB SO0142 0.88 +0.7 150,827 + +0.2 150,840 + -0.1 150,939 + -0.8 150,942 - -0.4 150,942 - +0.2 151,013 - +0.3 151,084 - +0.3 151,098 + +0.1 151,106 - +0.7 151,141 + -0.1 151,192 - +0.2 151,312 + -1.6 151,432 + +1.1 151,518 + +0.9 151,536 - -0.4 151,546 - -0.2 151,590 - +0.1 151,707 + -0.1 151,707 + -0.1 151,707 + +0.1 151,707 + +0.1 151,707 + -0.2 151,707 + +0.7 151,715 - +0.6 151,715 - -0.2 151,715 - +0.1 151,715 - -0.2 151,715 - -0.3 151,715 - -0.9 151,715 - +0.3 151,734 + -0.4 151,747 - +0.4 151,757 - +0.3 151,812 - ptrB SO0144 0.12 -0.2 151,841 + ptrB SO0144 0.14 +0.1 151,896 - ptrB SO0144 0.16 -0.2 151,925 + ptrB SO0144 0.18 +0.3 151,925 + ptrB SO0144 0.18 -0.2 151,937 - ptrB SO0144 0.18 +0.4 152,025 - ptrB SO0144 0.22 -0.5 152,100 + ptrB SO0144 0.26 +0.9 152,108 - ptrB SO0144 0.26 +0.4 152,136 + ptrB SO0144 0.28 +0.1 152,203 + ptrB SO0144 0.31 +1.5 152,211 - ptrB SO0144 0.31 +0.1 152,218 - ptrB SO0144 0.32 -1.3 152,231 - ptrB SO0144 0.32 +0.2 152,231 - ptrB SO0144 0.32 +0.2 152,248 + ptrB SO0144 0.33 +0.8 152,270 + ptrB SO0144 0.34 +0.2 152,278 - ptrB SO0144 0.35 +0.3 152,347 + ptrB SO0144 0.38 -0.7 152,348 + ptrB SO0144 0.38 +0.3 152,348 + ptrB SO0144 0.38 +0.2 152,356 - ptrB SO0144 0.38 +0.4 152,358 + ptrB SO0144 0.38 -0.2 152,358 + ptrB SO0144 0.38 +0.6 152,366 - ptrB SO0144 0.39 -0.3 152,402 + ptrB SO0144 0.40 +1.0 152,549 + ptrB SO0144 0.47 +0.7 152,583 - ptrB SO0144 0.49 -0.0 152,612 - ptrB SO0144 0.50 -0.1 152,675 + ptrB SO0144 0.53 -0.2 152,728 + ptrB SO0144 0.56 +0.3 152,728 + ptrB SO0144 0.56 -0.4 152,768 - ptrB SO0144 0.58 +0.4 152,801 + ptrB SO0144 0.59 +0.3 152,870 + ptrB SO0144 0.63 +5.5 153,022 + ptrB SO0144 0.70 -0.1 153,150 + ptrB SO0144 0.76 -0.7 153,185 - ptrB SO0144 0.78 +0.6 153,194 + ptrB SO0144 0.78 +0.6 153,232 + ptrB SO0144 0.80 +0.4 153,279 + ptrB SO0144 0.82 +0.0 153,298 - ptrB SO0144 0.83 -0.1 153,393 - ptrB SO0144 0.88 +0.6 153,393 - ptrB SO0144 0.88 +0.2 153,393 - ptrB SO0144 0.88 +0.5 153,436 + ptrB SO0144 0.90 +0.4 153,436 + ptrB SO0144 0.90 +1.2 153,436 + ptrB SO0144 0.90 -0.2 153,446 + +0.4 153,602 - -0.4 153,626 + +0.4 153,657 + -0.2 153,701 + +0.0 153,747 + SO0145 0.19 +0.3 153,747 + SO0145 0.19 -0.5 153,748 - SO0145 0.19 +1.0 153,803 - SO0145 0.34 -0.1 153,880 - SO0145 0.55 -0.9 153,965 + SO0145 0.78 +0.5 153,973 - SO0145 0.80 +0.0 154,039 - +0.0 154,096 - -0.0
Or see this region's nucleotide sequence