Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplQ and SO0258 are separated by 348 nucleotides SO0258 and SO0258.1 are separated by 254 nucleotides SO0258.1 and ccmE are separated by 387 nucleotides ccmE and ccmD overlap by 4 nucleotides
SO0257: rplQ - ribosomal protein L17 (NCBI ptt file), at 258,793 to 259,188
rplQ
SO0258: SO0258 - hypothetical protein (NCBI ptt file), at 259,537 to 259,692
SO0258
SO0258.1: SO0258.1 - putative transposase (NCBI ptt file), at 259,947 to 260,153
SO0258.1
SO0259: ccmE - cytochrome c biogenesis protein CcmE (NCBI ptt file), at 260,541 to 261,026
ccmE
SO0260: ccmD - heme exporter protein CcmD (NCBI ptt file), at 261,023 to 261,223
ccmD
Position (kb) 259
260
261 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 259.470 kb on + strand at 259.470 kb on + strand at 259.478 kb on - strand at 259.537 kb on + strand at 259.545 kb on - strand at 259.700 kb on + strand at 259.700 kb on + strand at 259.978 kb on + strand, within SO0258.1 at 260.017 kb on + strand, within SO0258.1 at 260.058 kb on - strand, within SO0258.1 at 260.067 kb on + strand, within SO0258.1 at 260.069 kb on + strand, within SO0258.1 at 260.148 kb on + strand at 260.189 kb on + strand at 260.197 kb on - strand at 260.218 kb on + strand at 260.430 kb on + strand at 260.471 kb on - strand at 260.646 kb on + strand, within ccmE at 260.821 kb on - strand, within ccmE at 260.858 kb on - strand, within ccmE at 261.000 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 259,470 + -0.4 259,470 + +0.1 259,478 - -0.3 259,537 + +0.7 259,545 - +0.0 259,700 + -0.3 259,700 + -1.8 259,978 + SO0258.1 0.15 +0.6 260,017 + SO0258.1 0.34 -0.0 260,058 - SO0258.1 0.54 +0.9 260,067 + SO0258.1 0.58 +1.7 260,069 + SO0258.1 0.59 -0.1 260,148 + +0.0 260,189 + +0.1 260,197 - -0.4 260,218 + -0.6 260,430 + -0.0 260,471 - +0.1 260,646 + ccmE SO0259 0.22 -1.4 260,821 - ccmE SO0259 0.58 +0.7 260,858 - ccmE SO0259 0.65 -2.8 261,000 - -0.7
Or see this region's nucleotide sequence