Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purB and SO2636 are separated by 126 nucleotides SO2636 and SO2637 are separated by 126 nucleotides SO2637 and ldh are separated by 280 nucleotides
SO2635: purB - adenylosuccinate lyase (NCBI ptt file), at 2,770,486 to 2,771,856
purB
SO2636: SO2636 - conserved hypothetical protein (NCBI ptt file), at 2,771,983 to 2,773,125
SO2636
SO2637: SO2637 - hypothetical protein (NCBI ptt file), at 2,773,252 to 2,773,704
SO2637
SO2638: ldh - leucine dehydrogenase (NCBI ptt file), at 2,773,985 to 2,775,019
ldh
Position (kb)
2771
2772
2773
2774 Strain fitness (log2 ratio)
-2
-1
0
1 at 2771.948 kb on - strand at 2772.370 kb on - strand, within SO2636 at 2772.412 kb on + strand, within SO2636 at 2772.430 kb on - strand, within SO2636 at 2772.727 kb on - strand, within SO2636 at 2772.777 kb on + strand, within SO2636 at 2772.885 kb on - strand, within SO2636 at 2772.896 kb on + strand, within SO2636 at 2772.996 kb on + strand, within SO2636 at 2773.090 kb on + strand at 2773.098 kb on - strand at 2773.243 kb on + strand at 2773.251 kb on - strand at 2773.251 kb on - strand at 2773.630 kb on - strand, within SO2637 at 2773.716 kb on + strand at 2773.882 kb on + strand at 2773.888 kb on - strand at 2773.890 kb on - strand at 2774.015 kb on - strand at 2774.107 kb on - strand, within ldh
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 2,771,948 - +0.3 2,772,370 - SO2636 0.34 +0.2 2,772,412 + SO2636 0.38 +0.6 2,772,430 - SO2636 0.39 -0.2 2,772,727 - SO2636 0.65 +1.5 2,772,777 + SO2636 0.69 -0.1 2,772,885 - SO2636 0.79 +0.3 2,772,896 + SO2636 0.80 -0.2 2,772,996 + SO2636 0.89 +0.9 2,773,090 + -0.2 2,773,098 - -0.3 2,773,243 + -0.0 2,773,251 - +0.2 2,773,251 - +0.4 2,773,630 - SO2637 0.83 -0.8 2,773,716 + +0.5 2,773,882 + +0.2 2,773,888 - +0.6 2,773,890 - -0.4 2,774,015 - +0.0 2,774,107 - ldh SO2638 0.12 -2.5
Or see this region's nucleotide sequence