Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2473 and SO2474 are separated by 168 nucleotides SO2474 and SO2476 are separated by 342 nucleotides
SO2473: SO2473 - hydrolase, alpha/beta fold family (NCBI ptt file), at 2,595,046 to 2,595,879
SO2473
SO2474: SO2474 - carbonic anhydrase family protein (NCBI ptt file), at 2,596,048 to 2,596,653
SO2474
SO2476: SO2476 - polysaccharide biosynthesis protein (NCBI ptt file), at 2,596,996 to 2,598,183
SO2476
Position (kb)
2596
2597 Strain fitness (log2 ratio)
-1
0
1
2 at 2595.096 kb on + strand at 2595.146 kb on + strand, within SO2473 at 2595.148 kb on + strand, within SO2473 at 2595.290 kb on + strand, within SO2473 at 2595.458 kb on + strand, within SO2473 at 2595.518 kb on - strand, within SO2473 at 2595.597 kb on - strand, within SO2473 at 2595.657 kb on + strand, within SO2473 at 2595.703 kb on + strand, within SO2473 at 2595.706 kb on - strand, within SO2473 at 2595.721 kb on - strand, within SO2473 at 2595.734 kb on - strand, within SO2473 at 2595.858 kb on - strand at 2595.908 kb on + strand at 2596.158 kb on + strand, within SO2474 at 2596.347 kb on - strand, within SO2474 at 2596.573 kb on + strand, within SO2474 at 2596.726 kb on - strand at 2596.848 kb on - strand at 2597.003 kb on + strand at 2597.068 kb on - strand at 2597.335 kb on + strand, within SO2476 at 2597.358 kb on - strand, within SO2476 at 2597.374 kb on + strand, within SO2476 at 2597.650 kb on + strand, within SO2476
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 2,595,096 + +0.3 2,595,146 + SO2473 0.12 +0.0 2,595,148 + SO2473 0.12 -0.9 2,595,290 + SO2473 0.29 +1.8 2,595,458 + SO2473 0.49 -0.3 2,595,518 - SO2473 0.57 -0.3 2,595,597 - SO2473 0.66 +0.0 2,595,657 + SO2473 0.73 +0.4 2,595,703 + SO2473 0.79 -0.5 2,595,706 - SO2473 0.79 +0.9 2,595,721 - SO2473 0.81 +0.2 2,595,734 - SO2473 0.82 -1.2 2,595,858 - -0.3 2,595,908 + +0.7 2,596,158 + SO2474 0.18 +1.2 2,596,347 - SO2474 0.49 +0.8 2,596,573 + SO2474 0.87 -0.4 2,596,726 - +0.3 2,596,848 - -0.4 2,597,003 + +1.1 2,597,068 - +1.0 2,597,335 + SO2476 0.29 -1.4 2,597,358 - SO2476 0.30 -0.0 2,597,374 + SO2476 0.32 -0.3 2,597,650 + SO2476 0.55 -1.2
Or see this region's nucleotide sequence