Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2010 and SO2011 are separated by 99 nucleotides SO2011 and apt are separated by 465 nucleotides apt and dnaX are separated by 131 nucleotides
SO2010: SO2010 - hypothetical protein (NCBI ptt file), at 2,108,238 to 2,108,360
SO2010
SO2011: SO2011 - conserved hypothetical protein (NCBI ptt file), at 2,108,460 to 2,108,828
SO2011
SO2012: apt - adenine phosphoribosyltransferase (NCBI ptt file), at 2,109,294 to 2,109,839
apt
SO2013: dnaX - DNA polymerase III, gamma and tau subunits (NCBI ptt file), at 2,109,971 to 2,112,979
dnaX
Position (kb)
2109
2110 Strain fitness (log2 ratio)
-1
0
1 at 2108.396 kb on - strand at 2108.398 kb on + strand at 2108.509 kb on + strand, within SO2011 at 2108.509 kb on + strand, within SO2011 at 2108.509 kb on + strand, within SO2011 at 2108.532 kb on - strand, within SO2011 at 2108.540 kb on + strand, within SO2011 at 2108.716 kb on + strand, within SO2011 at 2108.841 kb on - strand at 2108.982 kb on - strand at 2109.148 kb on + strand at 2109.160 kb on + strand at 2109.185 kb on + strand at 2109.205 kb on + strand at 2109.314 kb on - strand at 2109.529 kb on + strand, within apt at 2109.608 kb on - strand, within apt at 2109.857 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 2,108,396 - +0.5 2,108,398 + -0.0 2,108,509 + SO2011 0.13 +1.0 2,108,509 + SO2011 0.13 -0.5 2,108,509 + SO2011 0.13 +0.1 2,108,532 - SO2011 0.20 +1.1 2,108,540 + SO2011 0.22 -0.5 2,108,716 + SO2011 0.69 -0.4 2,108,841 - -0.1 2,108,982 - +0.0 2,109,148 + -0.4 2,109,160 + -0.9 2,109,185 + +0.9 2,109,205 + -0.6 2,109,314 - -0.2 2,109,529 + apt SO2012 0.43 -0.8 2,109,608 - apt SO2012 0.58 -1.2 2,109,857 + -0.5
Or see this region's nucleotide sequence