Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdeA and SO1813 are separated by 213 nucleotides SO1813 and SO1814 are separated by 471 nucleotides SO1814 and SO1815 are separated by 21 nucleotides
SO1812: mdeA - Methionine gamma-lyase (EC 4.4.1.11) (from data) , at 1,902,200 to 1,903,393
mdeA
SO1813: SO1813 - DNA-binding protein, putative (NCBI ptt file), at 1,903,607 to 1,903,972
SO1813
SO1814: SO1814 - hypothetical protein (NCBI ptt file), at 1,904,444 to 1,904,722
SO1814
SO1815: SO1815 - histone deacetylase/AcuC/AphA family protein (NCBI ptt file), at 1,904,744 to 1,905,658
SO1815
Position (kb)
1903
1904 Strain fitness (log2 ratio)
-1
0
1 at 1902.801 kb on + strand, within mdeA at 1902.820 kb on - strand, within mdeA at 1902.827 kb on + strand, within mdeA at 1902.887 kb on - strand, within mdeA at 1902.956 kb on - strand, within mdeA at 1902.986 kb on - strand, within mdeA at 1903.030 kb on - strand, within mdeA at 1903.045 kb on - strand, within mdeA at 1903.112 kb on + strand, within mdeA at 1903.148 kb on - strand, within mdeA at 1903.148 kb on - strand, within mdeA at 1903.332 kb on + strand at 1903.332 kb on + strand at 1903.369 kb on - strand at 1903.505 kb on - strand at 1903.720 kb on - strand, within SO1813 at 1903.786 kb on + strand, within SO1813 at 1903.849 kb on + strand, within SO1813 at 1903.917 kb on - strand, within SO1813 at 1903.993 kb on - strand at 1904.892 kb on - strand, within SO1815 at 1904.897 kb on - strand, within SO1815 at 1904.899 kb on + strand, within SO1815 at 1904.907 kb on - strand, within SO1815 at 1904.926 kb on - strand, within SO1815 at 1904.930 kb on + strand, within SO1815 at 1904.972 kb on - strand, within SO1815
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,902,801 + mdeA SO1812 0.50 -0.1 1,902,820 - mdeA SO1812 0.52 -0.5 1,902,827 + mdeA SO1812 0.53 -0.1 1,902,887 - mdeA SO1812 0.58 +0.3 1,902,956 - mdeA SO1812 0.63 -0.5 1,902,986 - mdeA SO1812 0.66 +1.4 1,903,030 - mdeA SO1812 0.70 -0.4 1,903,045 - mdeA SO1812 0.71 +0.0 1,903,112 + mdeA SO1812 0.76 -0.3 1,903,148 - mdeA SO1812 0.79 -0.2 1,903,148 - mdeA SO1812 0.79 +0.1 1,903,332 + -0.1 1,903,332 + +0.4 1,903,369 - -0.8 1,903,505 - -0.6 1,903,720 - SO1813 0.31 +0.2 1,903,786 + SO1813 0.49 +1.0 1,903,849 + SO1813 0.66 -0.1 1,903,917 - SO1813 0.85 +0.7 1,903,993 - -0.4 1,904,892 - SO1815 0.16 -1.0 1,904,897 - SO1815 0.17 -0.6 1,904,899 + SO1815 0.17 +0.4 1,904,907 - SO1815 0.18 +0.5 1,904,926 - SO1815 0.20 -0.5 1,904,930 + SO1815 0.20 +0.5 1,904,972 - SO1815 0.25 -1.1
Or see this region's nucleotide sequence