Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapD and sapC are separated by 3 nucleotides sapC and sapB overlap by 14 nucleotides sapB and sapA overlap by 1 nucleotides
SO1802: sapD - peptide ABC transporter, ATP-binding protein (NCBI ptt file), at 1,891,945 to 1,892,952
sapD
SO1803: sapC - peptide ABC transporter, permease protein (NCBI ptt file), at 1,892,956 to 1,893,846
sapC
SO1804: sapB - peptide ABC transporter, permease protein (NCBI ptt file), at 1,893,833 to 1,894,864
sapB
SO1805: sapA - peptide ABC transporter, periplasmic peptide-binding protein (NCBI ptt file), at 1,894,864 to 1,896,441
sapA
Position (kb)
1893
1894
1895 Strain fitness (log2 ratio)
-2
-1
0
1 at 1893.053 kb on + strand, within sapC at 1893.067 kb on + strand, within sapC at 1893.527 kb on - strand, within sapC at 1893.535 kb on + strand, within sapC at 1893.751 kb on - strand, within sapC at 1894.134 kb on + strand, within sapB at 1894.137 kb on - strand, within sapB at 1894.212 kb on - strand, within sapB at 1894.710 kb on + strand, within sapB at 1894.747 kb on - strand, within sapB at 1894.753 kb on + strand, within sapB at 1894.761 kb on - strand at 1894.761 kb on - strand at 1894.778 kb on - strand at 1894.984 kb on - strand at 1894.991 kb on + strand at 1895.567 kb on - strand, within sapA
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,893,053 + sapC SO1803 0.11 -1.1 1,893,067 + sapC SO1803 0.12 -0.8 1,893,527 - sapC SO1803 0.64 -1.1 1,893,535 + sapC SO1803 0.65 -0.6 1,893,751 - sapC SO1803 0.89 -0.1 1,894,134 + sapB SO1804 0.29 -0.6 1,894,137 - sapB SO1804 0.29 -0.7 1,894,212 - sapB SO1804 0.37 +0.0 1,894,710 + sapB SO1804 0.85 -0.1 1,894,747 - sapB SO1804 0.89 -0.1 1,894,753 + sapB SO1804 0.89 -1.5 1,894,761 - +0.9 1,894,761 - -2.4 1,894,778 - -0.3 1,894,984 - -0.1 1,894,991 + +0.2 1,895,567 - sapA SO1805 0.45 -1.7
Or see this region's nucleotide sequence