Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1512 and SO1513 are separated by 77 nucleotides SO1513 and SO1514 are separated by 364 nucleotides SO1514 and SO1515 are separated by 59 nucleotides
SO1512: SO1512 - hypothetical protein (NCBI ptt file), at 1,587,415 to 1,588,062
SO1512
SO1513: SO1513 - hypothetical protein (NCBI ptt file), at 1,588,140 to 1,588,604
SO1513
SO1514: SO1514 - ISSod1, transposase OrfA (NCBI ptt file), at 1,588,969 to 1,589,259
SO1514
SO1515: SO1515 - ISSod1, transposase OrfB (NCBI ptt file), at 1,589,319 to 1,590,128
SO1515
Position (kb)
1588
1589 Strain fitness (log2 ratio)
-1
0
1 at 1587.565 kb on + strand, within SO1512 at 1587.574 kb on - strand, within SO1512 at 1587.584 kb on + strand, within SO1512 at 1587.584 kb on + strand, within SO1512 at 1587.584 kb on + strand, within SO1512 at 1587.592 kb on - strand, within SO1512 at 1587.592 kb on - strand, within SO1512 at 1587.592 kb on - strand, within SO1512 at 1587.623 kb on + strand, within SO1512 at 1587.631 kb on - strand, within SO1512 at 1587.674 kb on + strand, within SO1512 at 1587.682 kb on - strand, within SO1512 at 1587.713 kb on + strand, within SO1512 at 1587.713 kb on + strand, within SO1512 at 1587.713 kb on + strand, within SO1512 at 1587.713 kb on + strand, within SO1512 at 1587.713 kb on + strand, within SO1512 at 1587.723 kb on + strand, within SO1512 at 1587.782 kb on + strand, within SO1512 at 1587.791 kb on + strand, within SO1512 at 1587.791 kb on + strand, within SO1512 at 1587.791 kb on + strand, within SO1512 at 1587.807 kb on + strand, within SO1512 at 1587.810 kb on - strand, within SO1512 at 1587.838 kb on - strand, within SO1512 at 1587.890 kb on + strand, within SO1512 at 1587.901 kb on - strand, within SO1512 at 1587.937 kb on - strand, within SO1512 at 1587.963 kb on + strand, within SO1512 at 1587.968 kb on + strand, within SO1512 at 1587.991 kb on - strand, within SO1512 at 1587.999 kb on - strand at 1588.096 kb on + strand at 1588.111 kb on - strand at 1588.117 kb on + strand at 1588.134 kb on + strand at 1588.182 kb on + strand at 1588.243 kb on + strand, within SO1513 at 1588.265 kb on + strand, within SO1513 at 1588.266 kb on + strand, within SO1513 at 1588.302 kb on + strand, within SO1513 at 1588.320 kb on - strand, within SO1513 at 1588.345 kb on - strand, within SO1513 at 1588.405 kb on - strand, within SO1513 at 1588.449 kb on + strand, within SO1513 at 1588.457 kb on - strand, within SO1513 at 1588.501 kb on + strand, within SO1513 at 1588.511 kb on - strand, within SO1513 at 1588.548 kb on + strand, within SO1513 at 1588.548 kb on + strand, within SO1513 at 1588.625 kb on - strand at 1588.673 kb on - strand at 1588.836 kb on + strand at 1588.842 kb on - strand at 1588.885 kb on + strand at 1588.896 kb on + strand at 1588.911 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand at 1588.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,587,565 + SO1512 0.23 -0.2 1,587,574 - SO1512 0.25 +0.6 1,587,584 + SO1512 0.26 -0.8 1,587,584 + SO1512 0.26 +0.4 1,587,584 + SO1512 0.26 -0.2 1,587,592 - SO1512 0.27 -0.1 1,587,592 - SO1512 0.27 -0.0 1,587,592 - SO1512 0.27 -0.3 1,587,623 + SO1512 0.32 +0.6 1,587,631 - SO1512 0.33 +0.2 1,587,674 + SO1512 0.40 -0.2 1,587,682 - SO1512 0.41 -1.2 1,587,713 + SO1512 0.46 -0.2 1,587,713 + SO1512 0.46 +0.3 1,587,713 + SO1512 0.46 -1.4 1,587,713 + SO1512 0.46 +0.5 1,587,713 + SO1512 0.46 +0.6 1,587,723 + SO1512 0.48 -0.3 1,587,782 + SO1512 0.57 -0.0 1,587,791 + SO1512 0.58 -0.2 1,587,791 + SO1512 0.58 +0.4 1,587,791 + SO1512 0.58 -1.3 1,587,807 + SO1512 0.60 -0.0 1,587,810 - SO1512 0.61 -0.1 1,587,838 - SO1512 0.65 -0.4 1,587,890 + SO1512 0.73 -0.2 1,587,901 - SO1512 0.75 +0.3 1,587,937 - SO1512 0.81 +0.7 1,587,963 + SO1512 0.85 -0.5 1,587,968 + SO1512 0.85 -0.1 1,587,991 - SO1512 0.89 +1.2 1,587,999 - -0.2 1,588,096 + -0.7 1,588,111 - +0.4 1,588,117 + +0.1 1,588,134 + -0.1 1,588,182 + -0.7 1,588,243 + SO1513 0.22 -0.1 1,588,265 + SO1513 0.27 +0.2 1,588,266 + SO1513 0.27 +0.9 1,588,302 + SO1513 0.35 +0.2 1,588,320 - SO1513 0.39 -0.8 1,588,345 - SO1513 0.44 -0.5 1,588,405 - SO1513 0.57 -0.1 1,588,449 + SO1513 0.66 +0.1 1,588,457 - SO1513 0.68 -0.6 1,588,501 + SO1513 0.78 -0.3 1,588,511 - SO1513 0.80 -0.2 1,588,548 + SO1513 0.88 -1.0 1,588,548 + SO1513 0.88 -0.6 1,588,625 - +0.1 1,588,673 - -0.2 1,588,836 + -0.5 1,588,842 - +0.5 1,588,885 + -0.2 1,588,896 + +0.6 1,588,911 - +0.3 1,588,916 - +0.3 1,588,916 - +0.0 1,588,916 - +0.1 1,588,916 - +0.3 1,588,916 - +1.0 1,588,916 - +0.8 1,588,916 - +0.2 1,588,916 - +0.1 1,588,916 - -0.4 1,588,916 - -0.0 1,588,916 - -0.1 1,588,916 - -0.5
Or see this region's nucleotide sequence