Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0997 and SO0998 overlap by 11 nucleotides SO0998 and pbpG are separated by 124 nucleotides pbpG and SO1000 are separated by 180 nucleotides
SO0997: SO0997 - transcriptional regulator, LysR family (NCBI ptt file), at 1,028,752 to 1,029,684
SO0997
SO0998: SO0998 - hypothetical protein (NCBI ptt file), at 1,029,674 to 1,029,853
SO0998
SO0999: pbpG - D-alanyl-D-alanine endopeptidase (NCBI ptt file), at 1,029,978 to 1,030,943
pbpG
SO1000: SO1000 - rhomboid family protein (NCBI ptt file), at 1,031,124 to 1,032,710
SO1000
Position (kb)
1029
1030
1031 Strain fitness (log2 ratio)
-2
-1
0
1 at 1029.066 kb on - strand, within SO0997 at 1029.098 kb on + strand, within SO0997 at 1029.106 kb on - strand, within SO0997 at 1029.106 kb on - strand, within SO0997 at 1029.223 kb on + strand, within SO0997 at 1029.223 kb on + strand, within SO0997 at 1029.230 kb on - strand, within SO0997 at 1029.231 kb on - strand, within SO0997 at 1029.236 kb on - strand, within SO0997 at 1029.557 kb on + strand, within SO0997 at 1029.628 kb on - strand at 1029.632 kb on - strand at 1029.896 kb on - strand at 1029.911 kb on + strand at 1030.024 kb on + strand at 1030.032 kb on - strand at 1030.032 kb on - strand at 1030.168 kb on - strand, within pbpG at 1030.257 kb on - strand, within pbpG at 1030.285 kb on + strand, within pbpG at 1030.429 kb on - strand, within pbpG at 1030.596 kb on + strand, within pbpG at 1030.753 kb on + strand, within pbpG at 1030.953 kb on - strand at 1031.070 kb on + strand at 1031.070 kb on + strand at 1031.070 kb on + strand at 1031.211 kb on + strand at 1031.211 kb on + strand at 1031.211 kb on + strand at 1031.219 kb on - strand at 1031.225 kb on + strand at 1031.238 kb on - strand at 1031.303 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.338 kb on + strand, within SO1000 at 1031.346 kb on - strand, within SO1000 at 1031.346 kb on - strand, within SO1000 at 1031.346 kb on - strand, within SO1000 at 1031.402 kb on - strand, within SO1000 at 1031.410 kb on - strand, within SO1000 at 1031.420 kb on - strand, within SO1000 at 1031.422 kb on - strand, within SO1000 at 1031.441 kb on + strand, within SO1000 at 1031.546 kb on + strand, within SO1000 at 1031.561 kb on + strand, within SO1000 at 1031.561 kb on + strand, within SO1000 at 1031.569 kb on - strand, within SO1000 at 1031.569 kb on - strand, within SO1000 at 1031.574 kb on - strand, within SO1000 at 1031.585 kb on + strand, within SO1000 at 1031.593 kb on - strand, within SO1000 at 1031.669 kb on + strand, within SO1000 at 1031.669 kb on + strand, within SO1000 at 1031.669 kb on + strand, within SO1000 at 1031.786 kb on + strand, within SO1000 at 1031.794 kb on - strand, within SO1000
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,029,066 - SO0997 0.34 +0.7 1,029,098 + SO0997 0.37 +0.4 1,029,106 - SO0997 0.38 -0.5 1,029,106 - SO0997 0.38 -1.1 1,029,223 + SO0997 0.50 +0.2 1,029,223 + SO0997 0.50 -0.3 1,029,230 - SO0997 0.51 +0.3 1,029,231 - SO0997 0.51 -0.8 1,029,236 - SO0997 0.52 -1.6 1,029,557 + SO0997 0.86 +0.9 1,029,628 - -0.4 1,029,632 - +0.1 1,029,896 - -1.9 1,029,911 + +0.3 1,030,024 + +0.0 1,030,032 - -0.4 1,030,032 - -0.0 1,030,168 - pbpG SO0999 0.20 +0.3 1,030,257 - pbpG SO0999 0.29 +0.5 1,030,285 + pbpG SO0999 0.32 +0.3 1,030,429 - pbpG SO0999 0.47 +0.4 1,030,596 + pbpG SO0999 0.64 +0.1 1,030,753 + pbpG SO0999 0.80 -0.0 1,030,953 - -1.3 1,031,070 + +0.1 1,031,070 + -0.2 1,031,070 + +0.2 1,031,211 + -1.0 1,031,211 + +0.7 1,031,211 + -0.0 1,031,219 - -0.7 1,031,225 + -0.1 1,031,238 - +0.2 1,031,303 + SO1000 0.11 +0.5 1,031,338 + SO1000 0.13 -0.2 1,031,338 + SO1000 0.13 +0.2 1,031,338 + SO1000 0.13 -0.2 1,031,338 + SO1000 0.13 +0.7 1,031,338 + SO1000 0.13 -0.6 1,031,338 + SO1000 0.13 -0.3 1,031,338 + SO1000 0.13 -0.1 1,031,338 + SO1000 0.13 -0.5 1,031,338 + SO1000 0.13 -0.3 1,031,346 - SO1000 0.14 +0.3 1,031,346 - SO1000 0.14 +0.8 1,031,346 - SO1000 0.14 -0.0 1,031,402 - SO1000 0.18 -0.2 1,031,410 - SO1000 0.18 +1.6 1,031,420 - SO1000 0.19 -0.7 1,031,422 - SO1000 0.19 +0.5 1,031,441 + SO1000 0.20 -0.1 1,031,546 + SO1000 0.27 +0.3 1,031,561 + SO1000 0.28 +0.6 1,031,561 + SO1000 0.28 -0.0 1,031,569 - SO1000 0.28 +0.7 1,031,569 - SO1000 0.28 +0.3 1,031,574 - SO1000 0.28 +0.1 1,031,585 + SO1000 0.29 +0.1 1,031,593 - SO1000 0.30 -0.2 1,031,669 + SO1000 0.34 -1.6 1,031,669 + SO1000 0.34 -0.4 1,031,669 + SO1000 0.34 -0.7 1,031,786 + SO1000 0.42 +1.4 1,031,794 - SO1000 0.42 -0.3
Or see this region's nucleotide sequence