Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0828 and SO0830 are separated by 214 nucleotides SO0830 and gshB are separated by 72 nucleotides
SO0828: SO0828 - conserved hypothetical protein (NCBI ptt file), at 846,823 to 847,884
SO0828
SO0830: SO0830 - alkaline phosphatase (NCBI ptt file), at 848,099 to 849,463
SO0830
SO0831: gshB - glutathione synthetase (NCBI ptt file), at 849,536 to 850,483
gshB
Position (kb) 848
849
850 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 847.175 kb on + strand, within SO0828 at 847.183 kb on - strand, within SO0828 at 847.187 kb on + strand, within SO0828 at 847.187 kb on + strand, within SO0828 at 847.195 kb on - strand, within SO0828 at 847.258 kb on - strand, within SO0828 at 847.282 kb on + strand, within SO0828 at 847.283 kb on - strand, within SO0828 at 847.283 kb on - strand, within SO0828 at 847.348 kb on + strand, within SO0828 at 847.373 kb on - strand, within SO0828 at 847.383 kb on + strand, within SO0828 at 847.390 kb on + strand, within SO0828 at 847.404 kb on + strand, within SO0828 at 847.423 kb on + strand, within SO0828 at 847.430 kb on - strand, within SO0828 at 847.430 kb on - strand, within SO0828 at 847.431 kb on - strand, within SO0828 at 847.431 kb on - strand, within SO0828 at 847.431 kb on - strand, within SO0828 at 847.431 kb on - strand, within SO0828 at 847.431 kb on - strand, within SO0828 at 847.521 kb on + strand, within SO0828 at 847.535 kb on + strand, within SO0828 at 847.580 kb on + strand, within SO0828 at 847.592 kb on + strand, within SO0828 at 847.600 kb on - strand, within SO0828 at 847.624 kb on - strand, within SO0828 at 847.636 kb on + strand, within SO0828 at 847.685 kb on + strand, within SO0828 at 847.695 kb on + strand, within SO0828 at 847.702 kb on + strand, within SO0828 at 847.739 kb on + strand, within SO0828 at 847.747 kb on - strand, within SO0828 at 847.800 kb on + strand at 847.838 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.843 kb on + strand at 847.848 kb on + strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.851 kb on - strand at 847.908 kb on - strand at 847.911 kb on + strand at 847.911 kb on + strand at 847.911 kb on + strand at 847.911 kb on + strand at 847.911 kb on + strand at 847.911 kb on + strand at 847.911 kb on + strand at 847.913 kb on + strand at 847.913 kb on + strand at 847.919 kb on - strand at 847.919 kb on - strand at 847.919 kb on - strand at 847.919 kb on - strand at 847.919 kb on - strand at 847.920 kb on - strand at 847.921 kb on - strand at 847.921 kb on - strand at 848.010 kb on + strand at 848.026 kb on + strand at 848.026 kb on + strand at 848.026 kb on + strand at 848.026 kb on + strand at 848.026 kb on + strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.034 kb on - strand at 848.089 kb on - strand at 848.097 kb on + strand at 848.097 kb on + strand at 848.097 kb on + strand at 848.105 kb on - strand at 848.105 kb on - strand at 848.105 kb on - strand at 848.105 kb on - strand at 848.124 kb on - strand at 848.133 kb on + strand at 848.212 kb on + strand at 848.220 kb on - strand at 848.271 kb on + strand, within SO0830 at 848.271 kb on + strand, within SO0830 at 848.317 kb on + strand, within SO0830 at 848.356 kb on + strand, within SO0830 at 848.380 kb on + strand, within SO0830 at 848.380 kb on + strand, within SO0830 at 848.426 kb on + strand, within SO0830 at 848.434 kb on - strand, within SO0830 at 848.529 kb on + strand, within SO0830 at 848.537 kb on - strand, within SO0830 at 848.537 kb on - strand, within SO0830 at 848.539 kb on - strand, within SO0830 at 848.544 kb on - strand, within SO0830 at 848.551 kb on + strand, within SO0830 at 848.559 kb on - strand, within SO0830 at 848.601 kb on + strand, within SO0830 at 848.673 kb on - strand, within SO0830 at 848.689 kb on + strand, within SO0830 at 848.689 kb on + strand, within SO0830 at 848.689 kb on + strand, within SO0830 at 848.697 kb on - strand, within SO0830 at 848.791 kb on - strand, within SO0830 at 848.919 kb on + strand, within SO0830 at 848.952 kb on + strand, within SO0830 at 848.952 kb on + strand, within SO0830 at 849.051 kb on - strand, within SO0830 at 849.086 kb on + strand, within SO0830 at 849.181 kb on + strand, within SO0830 at 849.300 kb on - strand, within SO0830 at 849.307 kb on + strand, within SO0830 at 849.356 kb on + strand at 849.389 kb on - strand at 849.463 kb on + strand at 849.466 kb on - strand at 849.526 kb on - strand at 849.708 kb on - strand, within gshB at 849.754 kb on - strand, within gshB at 849.811 kb on + strand, within gshB at 849.820 kb on - strand, within gshB at 849.926 kb on + strand, within gshB at 850.333 kb on + strand, within gshB at 850.397 kb on - strand at 850.462 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 847,175 + SO0828 0.33 -0.5 847,183 - SO0828 0.34 -0.3 847,187 + SO0828 0.34 -0.2 847,187 + SO0828 0.34 -0.1 847,195 - SO0828 0.35 -0.3 847,258 - SO0828 0.41 -0.4 847,282 + SO0828 0.43 -0.4 847,283 - SO0828 0.43 -0.2 847,283 - SO0828 0.43 +0.0 847,348 + SO0828 0.49 +0.0 847,373 - SO0828 0.52 -0.3 847,383 + SO0828 0.53 +0.3 847,390 + SO0828 0.53 -0.1 847,404 + SO0828 0.55 +0.3 847,423 + SO0828 0.56 +0.5 847,430 - SO0828 0.57 -0.1 847,430 - SO0828 0.57 -0.3 847,431 - SO0828 0.57 +0.1 847,431 - SO0828 0.57 -0.4 847,431 - SO0828 0.57 -0.5 847,431 - SO0828 0.57 -0.4 847,431 - SO0828 0.57 -0.2 847,521 + SO0828 0.66 +0.8 847,535 + SO0828 0.67 +0.2 847,580 + SO0828 0.71 -0.9 847,592 + SO0828 0.72 +0.8 847,600 - SO0828 0.73 +0.1 847,624 - SO0828 0.75 +0.8 847,636 + SO0828 0.77 -0.1 847,685 + SO0828 0.81 -0.5 847,695 + SO0828 0.82 +1.1 847,702 + SO0828 0.83 -1.1 847,739 + SO0828 0.86 +0.0 847,747 - SO0828 0.87 -0.1 847,800 + +0.2 847,838 + -0.2 847,843 + +0.8 847,843 + -0.7 847,843 + -0.1 847,843 + -0.1 847,843 + -0.3 847,843 + -1.2 847,843 + -0.7 847,843 + +0.1 847,843 + -0.5 847,843 + +0.2 847,843 + +0.5 847,848 + +0.0 847,851 - -0.0 847,851 - +0.0 847,851 - -0.5 847,851 - -0.2 847,851 - +0.6 847,851 - -0.6 847,851 - -1.0 847,851 - +0.3 847,851 - -0.0 847,851 - +0.2 847,851 - +0.1 847,851 - +0.3 847,851 - -1.1 847,851 - +0.3 847,908 - +0.4 847,911 + -0.3 847,911 + +0.2 847,911 + -0.3 847,911 + +0.6 847,911 + -0.1 847,911 + +1.1 847,911 + +0.2 847,913 + -0.3 847,913 + +1.0 847,919 - -0.2 847,919 - -0.1 847,919 - +0.5 847,919 - -0.6 847,919 - -0.5 847,920 - +0.7 847,921 - +0.1 847,921 - +0.1 848,010 + +0.1 848,026 + -0.1 848,026 + -2.2 848,026 + -0.5 848,026 + +0.2 848,026 + -0.2 848,034 - -0.7 848,034 - +0.5 848,034 - +0.1 848,034 - +0.3 848,034 - +0.1 848,034 - -0.6 848,034 - +0.3 848,034 - +0.1 848,034 - +0.4 848,034 - -0.2 848,034 - -0.1 848,034 - +0.1 848,034 - +0.5 848,034 - -0.8 848,034 - +0.2 848,034 - -0.2 848,089 - -0.8 848,097 + +0.2 848,097 + -0.0 848,097 + +0.6 848,105 - -1.2 848,105 - -0.2 848,105 - +0.0 848,105 - +0.2 848,124 - +0.3 848,133 + +0.1 848,212 + -0.2 848,220 - +0.1 848,271 + SO0830 0.13 -0.1 848,271 + SO0830 0.13 +0.2 848,317 + SO0830 0.16 +0.1 848,356 + SO0830 0.19 +0.6 848,380 + SO0830 0.21 -0.8 848,380 + SO0830 0.21 -0.6 848,426 + SO0830 0.24 -0.0 848,434 - SO0830 0.25 -0.2 848,529 + SO0830 0.32 -0.2 848,537 - SO0830 0.32 +0.1 848,537 - SO0830 0.32 +0.6 848,539 - SO0830 0.32 -0.7 848,544 - SO0830 0.33 +0.1 848,551 + SO0830 0.33 -0.1 848,559 - SO0830 0.34 +0.1 848,601 + SO0830 0.37 -0.2 848,673 - SO0830 0.42 -0.5 848,689 + SO0830 0.43 +0.2 848,689 + SO0830 0.43 -0.0 848,689 + SO0830 0.43 -1.0 848,697 - SO0830 0.44 -0.2 848,791 - SO0830 0.51 +0.1 848,919 + SO0830 0.60 +0.6 848,952 + SO0830 0.62 +0.6 848,952 + SO0830 0.62 -0.7 849,051 - SO0830 0.70 -0.2 849,086 + SO0830 0.72 +0.2 849,181 + SO0830 0.79 -0.3 849,300 - SO0830 0.88 +0.3 849,307 + SO0830 0.88 -0.0 849,356 + +0.4 849,389 - +0.0 849,463 + -0.4 849,466 - +0.8 849,526 - +0.2 849,708 - gshB SO0831 0.18 -3.1 849,754 - gshB SO0831 0.23 -3.6 849,811 + gshB SO0831 0.29 -3.4 849,820 - gshB SO0831 0.30 -4.4 849,926 + gshB SO0831 0.41 -3.0 850,333 + gshB SO0831 0.84 -4.2 850,397 - -2.2 850,462 + -1.2
Or see this region's nucleotide sequence