Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mrcB and ppiC-1 are separated by 77 nucleotides ppiC-1 and SO0636 are separated by 97 nucleotides SO0636 and SO0637 overlap by 4 nucleotides SO0637 and SO0638 are separated by 27 nucleotides
SO0633: mrcB - penicillin-binding protein 1B (NCBI ptt file), at 660,661 to 662,967
mrcB
SO0635: ppiC-1 - peptidyl-prolyl cis-trans isomerase C (NCBI ptt file), at 663,045 to 663,323
ppiC-1
SO0636: SO0636 - acetyltransferase, GNAT family (NCBI ptt file), at 663,421 to 663,915
SO0636
SO0637: SO0637 - conserved hypothetical protein (NCBI ptt file), at 663,912 to 664,187
SO0637
SO0638: SO0638 - hypothetical protein (NCBI ptt file), at 664,215 to 664,415
SO0638
Position (kb) 663
664 Strain fitness (log2 ratio) -2
-1
0
1 at 662.185 kb on + strand, within mrcB at 662.234 kb on - strand, within mrcB at 662.324 kb on + strand, within mrcB at 662.410 kb on + strand, within mrcB at 662.436 kb on + strand, within mrcB at 662.465 kb on - strand, within mrcB at 662.475 kb on + strand, within mrcB at 662.565 kb on - strand, within mrcB at 662.611 kb on - strand, within mrcB at 662.663 kb on + strand, within mrcB at 662.673 kb on + strand, within mrcB at 662.803 kb on - strand at 662.868 kb on + strand at 662.903 kb on + strand at 663.090 kb on - strand, within ppiC-1 at 663.200 kb on + strand, within ppiC-1 at 663.432 kb on - strand at 663.475 kb on + strand, within SO0636 at 663.485 kb on - strand, within SO0636 at 663.519 kb on - strand, within SO0636 at 663.588 kb on + strand, within SO0636 at 663.732 kb on + strand, within SO0636 at 663.930 kb on + strand at 664.114 kb on + strand, within SO0637 at 664.265 kb on + strand, within SO0638 at 664.293 kb on + strand, within SO0638
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 662,185 + mrcB SO0633 0.66 -2.1 662,234 - mrcB SO0633 0.68 -0.1 662,324 + mrcB SO0633 0.72 -1.3 662,410 + mrcB SO0633 0.76 -0.7 662,436 + mrcB SO0633 0.77 -1.1 662,465 - mrcB SO0633 0.78 -1.8 662,475 + mrcB SO0633 0.79 +0.3 662,565 - mrcB SO0633 0.83 -1.2 662,611 - mrcB SO0633 0.85 -0.5 662,663 + mrcB SO0633 0.87 -0.8 662,673 + mrcB SO0633 0.87 -0.6 662,803 - +0.3 662,868 + -0.8 662,903 + -1.4 663,090 - ppiC-1 SO0635 0.16 +0.2 663,200 + ppiC-1 SO0635 0.56 +0.5 663,432 - +0.4 663,475 + SO0636 0.11 -0.4 663,485 - SO0636 0.13 -0.2 663,519 - SO0636 0.20 +0.1 663,588 + SO0636 0.34 -0.1 663,732 + SO0636 0.63 -0.2 663,930 + -0.1 664,114 + SO0637 0.73 -0.7 664,265 + SO0638 0.25 +0.0 664,293 + SO0638 0.39 +0.5
Or see this region's nucleotide sequence